Evaluation of Substrates in the Elaboration of Secondary Inoculum for the Cultivation of Pleurotus ostreatus
The preparation of spawn or inoculum and its subsequent cultivation form the first step in the oyster mushroom supply chain, the preparation of the inoculum is related to quality parameters in the edible fungi production, where the use of grains of different cereals is essential to obtain the spawn...
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Journal of Pure and Applied Microbiology
2018-06-01
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Series: | Journal of Pure and Applied Microbiology |
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Online Access: | https://microbiologyjournal.org/evaluation-of-substrates-in-the-elaboration-of-secondary-inoculum-for-the-cultivation-of-pleurotus-ostreatus/ |
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author | Ma. Angeles Valencia de Ita Dulce P. Briones Aranda Conrado Parraguirre Lezama J. Refugio Tobar Reyes Armando Ibañes Martinez Omar Romero-Arenas |
author_facet | Ma. Angeles Valencia de Ita Dulce P. Briones Aranda Conrado Parraguirre Lezama J. Refugio Tobar Reyes Armando Ibañes Martinez Omar Romero-Arenas |
author_sort | Ma. Angeles Valencia de Ita |
collection | DOAJ |
description | The preparation of spawn or inoculum and its subsequent cultivation form the first step in the oyster mushroom supply chain, the preparation of the inoculum is related to quality parameters in the edible fungi production, where the use of grains of different cereals is essential to obtain the spawn The aim of this study was to determine the quality of different formulations of spawn using grains as wheat, corn, rice and sorghum furthermore, the growth of the strain of the CP-50 of Pleurotus ostreatus for oyster mushroom production on barley straw, under rustic conditions. In the spawn colonization and the percentage of contamination statistically significant differences were observed (P <0.05), being the treatments based on sorghum in combination with wheat, corn and rice the first to complete this process without presenting an incidence of competing organisms. Regarding yield and biological efficiency, no statistically significant differences (P <0.05) were found when using different formulations of spawn, values ranging from 84.17% were observed for fructification with the wheat + rice formulation, up to 71.45% when the formulation of spawn based on sorghum + corn was used. The production cycles had a variation of up to ten days between treatments, obtaining a more cut cycle when the sorghum + corn formulation was used. The results suggest the feasibility of using lower cost grains such as sorghum which impact in this process translates into a better cost benefit relation for small producers. |
first_indexed | 2024-04-11T19:26:45Z |
format | Article |
id | doaj.art-bda8665ab91645919036b469c48455b3 |
institution | Directory Open Access Journal |
issn | 0973-7510 2581-690X |
language | English |
last_indexed | 2024-04-11T19:26:45Z |
publishDate | 2018-06-01 |
publisher | Journal of Pure and Applied Microbiology |
record_format | Article |
series | Journal of Pure and Applied Microbiology |
spelling | doaj.art-bda8665ab91645919036b469c48455b32022-12-22T04:07:08ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2018-06-0112267968610.22207/JPAM.12.2.26Evaluation of Substrates in the Elaboration of Secondary Inoculum for the Cultivation of Pleurotus ostreatusMa. Angeles Valencia de Ita0Dulce P. Briones Aranda1Conrado Parraguirre Lezama2J. Refugio Tobar Reyes3Armando Ibañes Martinez4Omar Romero-Arenas5 Centro de Agroecología, Benemérita Universidad Autónoma de Puebla (BUAP). Facultad de Ingeniería Agrohidraulica-BUAP. Centro de Agroecología, Benemérita Universidad Autónoma de Puebla (BUAP). Facultad de Ingeniería Agrohidraulica-BUAP. Facultad de Ingeniería Agrohidraulica-BUAP. Centro de Agroecología, Benemérita Universidad Autónoma de Puebla (BUAP).The preparation of spawn or inoculum and its subsequent cultivation form the first step in the oyster mushroom supply chain, the preparation of the inoculum is related to quality parameters in the edible fungi production, where the use of grains of different cereals is essential to obtain the spawn The aim of this study was to determine the quality of different formulations of spawn using grains as wheat, corn, rice and sorghum furthermore, the growth of the strain of the CP-50 of Pleurotus ostreatus for oyster mushroom production on barley straw, under rustic conditions. In the spawn colonization and the percentage of contamination statistically significant differences were observed (P <0.05), being the treatments based on sorghum in combination with wheat, corn and rice the first to complete this process without presenting an incidence of competing organisms. Regarding yield and biological efficiency, no statistically significant differences (P <0.05) were found when using different formulations of spawn, values ranging from 84.17% were observed for fructification with the wheat + rice formulation, up to 71.45% when the formulation of spawn based on sorghum + corn was used. The production cycles had a variation of up to ten days between treatments, obtaining a more cut cycle when the sorghum + corn formulation was used. The results suggest the feasibility of using lower cost grains such as sorghum which impact in this process translates into a better cost benefit relation for small producers.https://microbiologyjournal.org/evaluation-of-substrates-in-the-elaboration-of-secondary-inoculum-for-the-cultivation-of-pleurotus-ostreatus/substratesbiological efficiencyspawn productioncereal grains. |
spellingShingle | Ma. Angeles Valencia de Ita Dulce P. Briones Aranda Conrado Parraguirre Lezama J. Refugio Tobar Reyes Armando Ibañes Martinez Omar Romero-Arenas Evaluation of Substrates in the Elaboration of Secondary Inoculum for the Cultivation of Pleurotus ostreatus Journal of Pure and Applied Microbiology substrates biological efficiency spawn production cereal grains. |
title | Evaluation of Substrates in the Elaboration of Secondary Inoculum for the Cultivation of Pleurotus ostreatus |
title_full | Evaluation of Substrates in the Elaboration of Secondary Inoculum for the Cultivation of Pleurotus ostreatus |
title_fullStr | Evaluation of Substrates in the Elaboration of Secondary Inoculum for the Cultivation of Pleurotus ostreatus |
title_full_unstemmed | Evaluation of Substrates in the Elaboration of Secondary Inoculum for the Cultivation of Pleurotus ostreatus |
title_short | Evaluation of Substrates in the Elaboration of Secondary Inoculum for the Cultivation of Pleurotus ostreatus |
title_sort | evaluation of substrates in the elaboration of secondary inoculum for the cultivation of pleurotus ostreatus |
topic | substrates biological efficiency spawn production cereal grains. |
url | https://microbiologyjournal.org/evaluation-of-substrates-in-the-elaboration-of-secondary-inoculum-for-the-cultivation-of-pleurotus-ostreatus/ |
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