A quantitative assessment method for Ascaris eggs on hands.
The importance of hands in the transmission of soil transmitted helminths, especially Ascaris and Trichuris infections, is under-researched. This is partly because of the absence of a reliable method to quantify the number of eggs on hands. Therefore, the aim of this study was to develop a method to...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4011755?pdf=render |
| _version_ | 1818479920193470464 |
|---|---|
| author | Aurelie Jeandron Jeroen H J Ensink Stig M Thamsborg Anders Dalsgaard Mita E Sengupta |
| author_facet | Aurelie Jeandron Jeroen H J Ensink Stig M Thamsborg Anders Dalsgaard Mita E Sengupta |
| author_sort | Aurelie Jeandron |
| collection | DOAJ |
| description | The importance of hands in the transmission of soil transmitted helminths, especially Ascaris and Trichuris infections, is under-researched. This is partly because of the absence of a reliable method to quantify the number of eggs on hands. Therefore, the aim of this study was to develop a method to assess the number of Ascaris eggs on hands and determine the egg recovery rate of the method. Under laboratory conditions, hands were seeded with a known number of Ascaris eggs, air dried and washed in a plastic bag retaining the washing water, in order to determine recovery rates of eggs for four different detergents (cationic [benzethonium chloride 0.1% and cetylpyridinium chloride CPC 0.1%], anionic [7X 1% - quadrafos, glycol ether, and dioctyl sulfoccinate sodium salt] and non-ionic [Tween80 0.1% -polyethylene glycol sorbitan monooleate]) and two egg detection methods (McMaster technique and FLOTAC). A modified concentration McMaster technique showed the highest egg recovery rate from bags. Two of the four diluted detergents (benzethonium chloride 0.1% and 7X 1%) also showed a higher egg recovery rate and were then compared with de-ionized water for recovery of helminth eggs from hands. The highest recovery rate (95.6%) was achieved with a hand rinse performed with 7X 1%. Washing hands with de-ionized water resulted in an egg recovery rate of 82.7%. This washing method performed with a low concentration of detergent offers potential for quantitative investigation of contamination of hands with Ascaris eggs and of their role in human infection. Follow-up studies are needed that validate the hand washing method under field conditions, e.g. including people of different age, lower levels of contamination and various levels of hand cleanliness. |
| first_indexed | 2024-12-10T11:16:24Z |
| format | Article |
| id | doaj.art-bda8f77a17074a8e8dac0e1a7ab9f02d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T11:16:24Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bda8f77a17074a8e8dac0e1a7ab9f02d2022-12-22T01:51:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0195e9673110.1371/journal.pone.0096731A quantitative assessment method for Ascaris eggs on hands.Aurelie JeandronJeroen H J EnsinkStig M ThamsborgAnders DalsgaardMita E SenguptaThe importance of hands in the transmission of soil transmitted helminths, especially Ascaris and Trichuris infections, is under-researched. This is partly because of the absence of a reliable method to quantify the number of eggs on hands. Therefore, the aim of this study was to develop a method to assess the number of Ascaris eggs on hands and determine the egg recovery rate of the method. Under laboratory conditions, hands were seeded with a known number of Ascaris eggs, air dried and washed in a plastic bag retaining the washing water, in order to determine recovery rates of eggs for four different detergents (cationic [benzethonium chloride 0.1% and cetylpyridinium chloride CPC 0.1%], anionic [7X 1% - quadrafos, glycol ether, and dioctyl sulfoccinate sodium salt] and non-ionic [Tween80 0.1% -polyethylene glycol sorbitan monooleate]) and two egg detection methods (McMaster technique and FLOTAC). A modified concentration McMaster technique showed the highest egg recovery rate from bags. Two of the four diluted detergents (benzethonium chloride 0.1% and 7X 1%) also showed a higher egg recovery rate and were then compared with de-ionized water for recovery of helminth eggs from hands. The highest recovery rate (95.6%) was achieved with a hand rinse performed with 7X 1%. Washing hands with de-ionized water resulted in an egg recovery rate of 82.7%. This washing method performed with a low concentration of detergent offers potential for quantitative investigation of contamination of hands with Ascaris eggs and of their role in human infection. Follow-up studies are needed that validate the hand washing method under field conditions, e.g. including people of different age, lower levels of contamination and various levels of hand cleanliness.http://europepmc.org/articles/PMC4011755?pdf=render |
| spellingShingle | Aurelie Jeandron Jeroen H J Ensink Stig M Thamsborg Anders Dalsgaard Mita E Sengupta A quantitative assessment method for Ascaris eggs on hands. PLoS ONE |
| title | A quantitative assessment method for Ascaris eggs on hands. |
| title_full | A quantitative assessment method for Ascaris eggs on hands. |
| title_fullStr | A quantitative assessment method for Ascaris eggs on hands. |
| title_full_unstemmed | A quantitative assessment method for Ascaris eggs on hands. |
| title_short | A quantitative assessment method for Ascaris eggs on hands. |
| title_sort | quantitative assessment method for ascaris eggs on hands |
| url | http://europepmc.org/articles/PMC4011755?pdf=render |
| work_keys_str_mv | AT aureliejeandron aquantitativeassessmentmethodforascariseggsonhands AT jeroenhjensink aquantitativeassessmentmethodforascariseggsonhands AT stigmthamsborg aquantitativeassessmentmethodforascariseggsonhands AT andersdalsgaard aquantitativeassessmentmethodforascariseggsonhands AT mitaesengupta aquantitativeassessmentmethodforascariseggsonhands AT aureliejeandron quantitativeassessmentmethodforascariseggsonhands AT jeroenhjensink quantitativeassessmentmethodforascariseggsonhands AT stigmthamsborg quantitativeassessmentmethodforascariseggsonhands AT andersdalsgaard quantitativeassessmentmethodforascariseggsonhands AT mitaesengupta quantitativeassessmentmethodforascariseggsonhands |