Comparative Analysis of Epididymis Cauda of Yak before and after Sexual Maturity
Epididymis development is the basis of male reproduction and is a crucial site where sperm maturation occurs. In order to further understand the epididymal development of yak and how to regulate sperm maturation, we conducted a multi-omics analysis. We detected 2274 differential genes, 222 different...
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MDPI AG
2023-04-01
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| author | Ziqiang Ding Lin Xiong Xingdong Wang Shaoke Guo Mengli Cao Yandong Kang Yongfu La Pengjia Bao Jie Pei Xian Guo |
| author_facet | Ziqiang Ding Lin Xiong Xingdong Wang Shaoke Guo Mengli Cao Yandong Kang Yongfu La Pengjia Bao Jie Pei Xian Guo |
| author_sort | Ziqiang Ding |
| collection | DOAJ |
| description | Epididymis development is the basis of male reproduction and is a crucial site where sperm maturation occurs. In order to further understand the epididymal development of yak and how to regulate sperm maturation, we conducted a multi-omics analysis. We detected 2274 differential genes, 222 differential proteins and 117 co-expression genes in the cauda epididymis of yak before and after sexual maturity by RNA-seq and proteomics techniques, which included TGFBI, COL1A1, COL1A2, COL3A1, COL12A1, SULT2B1, KRT19, and NPC2. These high abundance genes are mainly related to cell growth, differentiation, adhesion and sperm maturation, and are mainly enriched via extracellular matrix receptor interaction, protein differentiation and absorption, and lysosome and estrogen signaling pathways. The abnormal expression of these genes may lead to the retardation of epididymal cauda development and abnormal sperm function in yak. In conclusion, through single and combined analysis, we provided a theoretical basis for the development of the yak epididymal cauda, sperm maturation, and screening of key genes involved in the regulation of male yak reproduction. |
| first_indexed | 2024-03-11T05:19:16Z |
| format | Article |
| id | doaj.art-bda984cd0f6a49e6abc0ae62ce5260ce |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T05:19:16Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-bda984cd0f6a49e6abc0ae62ce5260ce2023-11-17T18:00:38ZengMDPI AGAnimals2076-26152023-04-01138135510.3390/ani13081355Comparative Analysis of Epididymis Cauda of Yak before and after Sexual MaturityZiqiang Ding0Lin Xiong1Xingdong Wang2Shaoke Guo3Mengli Cao4Yandong Kang5Yongfu La6Pengjia Bao7Jie Pei8Xian Guo9Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaEpididymis development is the basis of male reproduction and is a crucial site where sperm maturation occurs. In order to further understand the epididymal development of yak and how to regulate sperm maturation, we conducted a multi-omics analysis. We detected 2274 differential genes, 222 differential proteins and 117 co-expression genes in the cauda epididymis of yak before and after sexual maturity by RNA-seq and proteomics techniques, which included TGFBI, COL1A1, COL1A2, COL3A1, COL12A1, SULT2B1, KRT19, and NPC2. These high abundance genes are mainly related to cell growth, differentiation, adhesion and sperm maturation, and are mainly enriched via extracellular matrix receptor interaction, protein differentiation and absorption, and lysosome and estrogen signaling pathways. The abnormal expression of these genes may lead to the retardation of epididymal cauda development and abnormal sperm function in yak. In conclusion, through single and combined analysis, we provided a theoretical basis for the development of the yak epididymal cauda, sperm maturation, and screening of key genes involved in the regulation of male yak reproduction.https://www.mdpi.com/2076-2615/13/8/1355epididymisproteomicsRNA-seqsperm maturationyak |
| spellingShingle | Ziqiang Ding Lin Xiong Xingdong Wang Shaoke Guo Mengli Cao Yandong Kang Yongfu La Pengjia Bao Jie Pei Xian Guo Comparative Analysis of Epididymis Cauda of Yak before and after Sexual Maturity Animals epididymis proteomics RNA-seq sperm maturation yak |
| title | Comparative Analysis of Epididymis Cauda of Yak before and after Sexual Maturity |
| title_full | Comparative Analysis of Epididymis Cauda of Yak before and after Sexual Maturity |
| title_fullStr | Comparative Analysis of Epididymis Cauda of Yak before and after Sexual Maturity |
| title_full_unstemmed | Comparative Analysis of Epididymis Cauda of Yak before and after Sexual Maturity |
| title_short | Comparative Analysis of Epididymis Cauda of Yak before and after Sexual Maturity |
| title_sort | comparative analysis of epididymis cauda of yak before and after sexual maturity |
| topic | epididymis proteomics RNA-seq sperm maturation yak |
| url | https://www.mdpi.com/2076-2615/13/8/1355 |
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