Phloridzin Reveals New Treatment Strategies for Liver Fibrosis
Liver fibrosis is an urgent public health problem which is difficult to resolve. However, various drugs for the treatment of liver fibrosis in clinical practice have their own problems during use. In this study, we used phloridzin to treat hepatic fibrosis in the CCl<sub>4</sub>-induced...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/7/896 |
| _version_ | 1797416466880921600 |
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| author | Yahong Shi Tun Yan Xi Lu Kai Li Yifeng Nie Chuqiao Jiao Huizhen Sun Tingting Li Xiang Li Dong Han |
| author_facet | Yahong Shi Tun Yan Xi Lu Kai Li Yifeng Nie Chuqiao Jiao Huizhen Sun Tingting Li Xiang Li Dong Han |
| author_sort | Yahong Shi |
| collection | DOAJ |
| description | Liver fibrosis is an urgent public health problem which is difficult to resolve. However, various drugs for the treatment of liver fibrosis in clinical practice have their own problems during use. In this study, we used phloridzin to treat hepatic fibrosis in the CCl<sub>4</sub>-induced C57/BL6N mouse model, which was extracted from lychee core, a traditional Chinese medicine. The therapeutic effect was evaluated by biochemical index detections and ultrasound detection. Furthermore, in order to determine the mechanism of phloridzin in the treatment of liver fibrosis, we performed high-throughput sequencing of mRNA and lncRNA in different groups of liver tissues. The results showed that compared with the model group, the phloridzin-treated groups revealed a significant decrease in collagen deposition and decreased levels of serum alanine aminotransferase, aspartate aminotransferase, laminin, and hyaluronic acid. GO and KEGG pathway enrichment analysis of the differential mRNAs was performed and revealed that phloridzin mainly affects cell ferroptosis. Gene co-expression analysis showed that the target genes of lncRNA were obvious in cell components such as focal adhesions, intercellular adhesion, and cell–substrate junctions and in metabolic pathways such as carbon metabolism. These results showed that phloridizin can effectively treat liver fibrosis, and the mechanism may involve ferroptosis, carbon metabolism, and related changes in biomechanics. |
| first_indexed | 2024-03-09T06:04:40Z |
| format | Article |
| id | doaj.art-bdaae13d2f564655b48d2379af87ad75 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T06:04:40Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-bdaae13d2f564655b48d2379af87ad752023-12-03T12:05:50ZengMDPI AGPharmaceuticals1424-82472022-07-0115789610.3390/ph15070896Phloridzin Reveals New Treatment Strategies for Liver FibrosisYahong Shi0Tun Yan1Xi Lu2Kai Li3Yifeng Nie4Chuqiao Jiao5Huizhen Sun6Tingting Li7Xiang Li8Dong Han9School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, ChinaNational Center for Nanoscience and Technology, Beijing 100190, ChinaBeijing City International School, Beijing 100022, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, ChinaNational Center for Nanoscience and Technology, Beijing 100190, ChinaNational Center for Nanoscience and Technology, Beijing 100190, ChinaLiver fibrosis is an urgent public health problem which is difficult to resolve. However, various drugs for the treatment of liver fibrosis in clinical practice have their own problems during use. In this study, we used phloridzin to treat hepatic fibrosis in the CCl<sub>4</sub>-induced C57/BL6N mouse model, which was extracted from lychee core, a traditional Chinese medicine. The therapeutic effect was evaluated by biochemical index detections and ultrasound detection. Furthermore, in order to determine the mechanism of phloridzin in the treatment of liver fibrosis, we performed high-throughput sequencing of mRNA and lncRNA in different groups of liver tissues. The results showed that compared with the model group, the phloridzin-treated groups revealed a significant decrease in collagen deposition and decreased levels of serum alanine aminotransferase, aspartate aminotransferase, laminin, and hyaluronic acid. GO and KEGG pathway enrichment analysis of the differential mRNAs was performed and revealed that phloridzin mainly affects cell ferroptosis. Gene co-expression analysis showed that the target genes of lncRNA were obvious in cell components such as focal adhesions, intercellular adhesion, and cell–substrate junctions and in metabolic pathways such as carbon metabolism. These results showed that phloridizin can effectively treat liver fibrosis, and the mechanism may involve ferroptosis, carbon metabolism, and related changes in biomechanics.https://www.mdpi.com/1424-8247/15/7/896phloridzinliver fibrosismRNAlncRNAferroptosisenergy metabolism |
| spellingShingle | Yahong Shi Tun Yan Xi Lu Kai Li Yifeng Nie Chuqiao Jiao Huizhen Sun Tingting Li Xiang Li Dong Han Phloridzin Reveals New Treatment Strategies for Liver Fibrosis Pharmaceuticals phloridzin liver fibrosis mRNA lncRNA ferroptosis energy metabolism |
| title | Phloridzin Reveals New Treatment Strategies for Liver Fibrosis |
| title_full | Phloridzin Reveals New Treatment Strategies for Liver Fibrosis |
| title_fullStr | Phloridzin Reveals New Treatment Strategies for Liver Fibrosis |
| title_full_unstemmed | Phloridzin Reveals New Treatment Strategies for Liver Fibrosis |
| title_short | Phloridzin Reveals New Treatment Strategies for Liver Fibrosis |
| title_sort | phloridzin reveals new treatment strategies for liver fibrosis |
| topic | phloridzin liver fibrosis mRNA lncRNA ferroptosis energy metabolism |
| url | https://www.mdpi.com/1424-8247/15/7/896 |
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