Generation and analysis of expressed sequence tags from Botrytis cinerea
Botrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags th...
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| Format: | Article |
| Language: | English |
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BMC
2006-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200018 |
| _version_ | 1819051785616097280 |
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| author | EVELYN SILVA JORGE VALDÉS DAVID HOLMES AMIR SHMARYAHU PABLO D T VALENZUELA |
| author_facet | EVELYN SILVA JORGE VALDÉS DAVID HOLMES AMIR SHMARYAHU PABLO D T VALENZUELA |
| author_sort | EVELYN SILVA |
| collection | DOAJ |
| description | Botrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags that were assembled into 1,003 contigs sequences and 3,032 singletons. Approximately 81% of the unigenes showed significant similarity to genes coding for proteins with known functions: more than 50% of the sequences code for genes involved in cellular metabolism, 12% for transport of metabolites, and approximately 10% for cellular organization. Other functional categories include responses to biotic and abiotic stimuli, cell communication, cell homeostasis, and cell development. We carried out pair-wise comparisons with fungal databases to determine the B. cinerea unisequence set with relevant similarity to genes in other fungal pathogenic counterparts. Among the 4,035 non-redundant B. cinerea unigenes, 1,338 (23%) have significant homology with Fusarium verticillioides unigenes. Similar values were obtained for Saccharomyces cerevisiae and Aspergillus nidulans (22% and 24%, respectively). The lower percentages of homology were with Magnaporthe grisae and Neurospora crassa (13% and 19%, respectively). Several genes involved in putative and known fungal virulence and general pathogenicity were identified. The results provide important information for future research on this fungal pathogen |
| first_indexed | 2024-12-21T12:09:28Z |
| format | Article |
| id | doaj.art-bdad79994fbc4827b11777d9a679806a |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-12-21T12:09:28Z |
| publishDate | 2006-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-bdad79994fbc4827b11777d9a679806a2022-12-21T19:04:37ZengBMCBiological Research0716-97600717-62872006-01-01392367376Generation and analysis of expressed sequence tags from Botrytis cinereaEVELYN SILVAJORGE VALDÉSDAVID HOLMESAMIR SHMARYAHUPABLO D T VALENZUELABotrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags that were assembled into 1,003 contigs sequences and 3,032 singletons. Approximately 81% of the unigenes showed significant similarity to genes coding for proteins with known functions: more than 50% of the sequences code for genes involved in cellular metabolism, 12% for transport of metabolites, and approximately 10% for cellular organization. Other functional categories include responses to biotic and abiotic stimuli, cell communication, cell homeostasis, and cell development. We carried out pair-wise comparisons with fungal databases to determine the B. cinerea unisequence set with relevant similarity to genes in other fungal pathogenic counterparts. Among the 4,035 non-redundant B. cinerea unigenes, 1,338 (23%) have significant homology with Fusarium verticillioides unigenes. Similar values were obtained for Saccharomyces cerevisiae and Aspergillus nidulans (22% and 24%, respectively). The lower percentages of homology were with Magnaporthe grisae and Neurospora crassa (13% and 19%, respectively). Several genes involved in putative and known fungal virulence and general pathogenicity were identified. The results provide important information for future research on this fungal pathogenhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200018Botrytis cinereacDNA sequencingfungal pathogenicity |
| spellingShingle | EVELYN SILVA JORGE VALDÉS DAVID HOLMES AMIR SHMARYAHU PABLO D T VALENZUELA Generation and analysis of expressed sequence tags from Botrytis cinerea Biological Research Botrytis cinerea cDNA sequencing fungal pathogenicity |
| title | Generation and analysis of expressed sequence tags from Botrytis cinerea |
| title_full | Generation and analysis of expressed sequence tags from Botrytis cinerea |
| title_fullStr | Generation and analysis of expressed sequence tags from Botrytis cinerea |
| title_full_unstemmed | Generation and analysis of expressed sequence tags from Botrytis cinerea |
| title_short | Generation and analysis of expressed sequence tags from Botrytis cinerea |
| title_sort | generation and analysis of expressed sequence tags from botrytis cinerea |
| topic | Botrytis cinerea cDNA sequencing fungal pathogenicity |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200018 |
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