ACE gene dosage determines additional autonomic dysfunction and increases renal angiotensin II levels in diabetic mice
OBJECTIVES: The present study aimed to investigate cardiovascular autonomic modulation and angiotensin II (Ang II) activity in diabetic mice that were genetically engineered to harbor two or three copies of the angiotensin-converting enzyme gene. METHODS: Diabetic and non-diabetic mice harboring 2...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier España
2018-08-01
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| Series: | Clinics |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322018000100245&lng=en&tlng=en |
| _version_ | 1818522773549481984 |
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| author | Oscar Albuquerque de Moraes Karin Flues Kátia Bilhar Scapini Cristiano Mostarda Fabiana de Sant’Anna Evangelista Bruno Rodrigues Daniela Ravizzoni Dartora Patricia Fiorino Kátia De Angelis Maria Cláudia Irigoyen |
| author_facet | Oscar Albuquerque de Moraes Karin Flues Kátia Bilhar Scapini Cristiano Mostarda Fabiana de Sant’Anna Evangelista Bruno Rodrigues Daniela Ravizzoni Dartora Patricia Fiorino Kátia De Angelis Maria Cláudia Irigoyen |
| author_sort | Oscar Albuquerque de Moraes |
| collection | DOAJ |
| description | OBJECTIVES: The present study aimed to investigate cardiovascular autonomic modulation and angiotensin II (Ang II) activity in diabetic mice that were genetically engineered to harbor two or three copies of the angiotensin-converting enzyme gene. METHODS: Diabetic and non-diabetic mice harboring 2 or 3 copies of the angiotensin-converting enzyme gene were used in the present study. Animals were divided into 4 groups: diabetic groups with two and three copies of the angiotensin-converting enzyme gene (2CD and 3CD) and the respective age-matched non-diabetic groups (2C and 3C). Hemodynamic, cardiovascular, and autonomic parameters as well as renal Ang II expression were evaluated. RESULTS: Heart rate was lower in diabetic animals than in non-diabetic animals. Autonomic modulation analysis indicated that the 3CD group showed increased sympathetic modulation and decreased vagal modulation of heart rate variability, eliciting increased cardiac sympathovagal balance, compared with all the other groups. Concurrent diabetes and either angiotensin-converting enzyme polymorphism resulted in a significant increase in Ang II expression in the renal cortex. CONCLUSION: Data indicates that a small increase in angiotensin-converting enzyme activity in diabetic animals leads to greater impairment of autonomic function, as demonstrated by increased sympathetic modulation and reduced cardiac vagal modulation along with increased renal expression of Ang II. |
| first_indexed | 2024-12-11T05:37:26Z |
| format | Article |
| id | doaj.art-bdae615f9983438fa55b8c787ae4d022 |
| institution | Directory Open Access Journal |
| issn | 1980-5322 |
| language | English |
| last_indexed | 2024-12-11T05:37:26Z |
| publishDate | 2018-08-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-bdae615f9983438fa55b8c787ae4d0222022-12-22T01:19:15ZengElsevier EspañaClinics1980-53222018-08-0173010.6061/clinics/2018/e246S1807-59322018000100245ACE gene dosage determines additional autonomic dysfunction and increases renal angiotensin II levels in diabetic miceOscar Albuquerque de MoraesKarin FluesKátia Bilhar ScapiniCristiano MostardaFabiana de Sant’Anna EvangelistaBruno RodriguesDaniela Ravizzoni DartoraPatricia FiorinoKátia De AngelisMaria Cláudia IrigoyenOBJECTIVES: The present study aimed to investigate cardiovascular autonomic modulation and angiotensin II (Ang II) activity in diabetic mice that were genetically engineered to harbor two or three copies of the angiotensin-converting enzyme gene. METHODS: Diabetic and non-diabetic mice harboring 2 or 3 copies of the angiotensin-converting enzyme gene were used in the present study. Animals were divided into 4 groups: diabetic groups with two and three copies of the angiotensin-converting enzyme gene (2CD and 3CD) and the respective age-matched non-diabetic groups (2C and 3C). Hemodynamic, cardiovascular, and autonomic parameters as well as renal Ang II expression were evaluated. RESULTS: Heart rate was lower in diabetic animals than in non-diabetic animals. Autonomic modulation analysis indicated that the 3CD group showed increased sympathetic modulation and decreased vagal modulation of heart rate variability, eliciting increased cardiac sympathovagal balance, compared with all the other groups. Concurrent diabetes and either angiotensin-converting enzyme polymorphism resulted in a significant increase in Ang II expression in the renal cortex. CONCLUSION: Data indicates that a small increase in angiotensin-converting enzyme activity in diabetic animals leads to greater impairment of autonomic function, as demonstrated by increased sympathetic modulation and reduced cardiac vagal modulation along with increased renal expression of Ang II.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322018000100245&lng=en&tlng=enRenin-angiotensin SystemAutonomic Nervous SystemRenal Angiotensin SystemMice |
| spellingShingle | Oscar Albuquerque de Moraes Karin Flues Kátia Bilhar Scapini Cristiano Mostarda Fabiana de Sant’Anna Evangelista Bruno Rodrigues Daniela Ravizzoni Dartora Patricia Fiorino Kátia De Angelis Maria Cláudia Irigoyen ACE gene dosage determines additional autonomic dysfunction and increases renal angiotensin II levels in diabetic mice Clinics Renin-angiotensin System Autonomic Nervous System Renal Angiotensin System Mice |
| title | ACE gene dosage determines additional autonomic dysfunction and increases renal angiotensin II levels in diabetic mice |
| title_full | ACE gene dosage determines additional autonomic dysfunction and increases renal angiotensin II levels in diabetic mice |
| title_fullStr | ACE gene dosage determines additional autonomic dysfunction and increases renal angiotensin II levels in diabetic mice |
| title_full_unstemmed | ACE gene dosage determines additional autonomic dysfunction and increases renal angiotensin II levels in diabetic mice |
| title_short | ACE gene dosage determines additional autonomic dysfunction and increases renal angiotensin II levels in diabetic mice |
| title_sort | ace gene dosage determines additional autonomic dysfunction and increases renal angiotensin ii levels in diabetic mice |
| topic | Renin-angiotensin System Autonomic Nervous System Renal Angiotensin System Mice |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322018000100245&lng=en&tlng=en |
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