Cyclodextrin production from amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans CCM 2012
Cyclodextrins (CDs) are synthesised by bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives. The production of α-, β-, and γ-CDs by CGTase from Paenibacillus mace-rans CCM 2012 was studied in regard to the effect of the starch sourc...
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Czech Academy of Agricultural Sciences
2012-02-01
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| Series: | Czech Journal of Food Sciences |
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| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201201-0003_cyclodextrin-production-from-amaranth-starch-by-cyclodextrin-glycosyltransferase-produced-by-paenibacillus-mace.php |
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| author | Marian Urban Miloš Beran Lubomír Adámek Josef Drahorád Petr Molík Kristina Matušová |
| author_facet | Marian Urban Miloš Beran Lubomír Adámek Josef Drahorád Petr Molík Kristina Matušová |
| author_sort | Marian Urban |
| collection | DOAJ |
| description | Cyclodextrins (CDs) are synthesised by bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives. The production of α-, β-, and γ-CDs by CGTase from Paenibacillus mace-rans CCM 2012 was studied in regard to the effect of the starch source (amaranth, maize) on the yield of CDs. CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB under aerobic conditions. CGTase was partially purified by ammonium sulfate precipitation at 60% saturation. Electrophoretic analysis (SDS-PAGE) of the isolated CGTase enzyme was carried out according to the method by Laemmli (1970), the apparent molecular weight was in the range from 105 kDa to 114 kDa. All the commercially important α-, β-, and γ-CDs were detected chromatographically after the hydrolysis of the maize and amaranth (Amaranthus cruentus) starches with the isolated enzyme. The amaranth starch appears to be an excellent substrate for CDs production because of the high dispersibility, high starch-granule susceptibility to amylases, and the exceptionally high amylopectin content. |
| first_indexed | 2024-04-10T08:34:08Z |
| format | Article |
| id | doaj.art-bdafebb96d3a4f07b032ee956b0c9455 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:34:08Z |
| publishDate | 2012-02-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-bdafebb96d3a4f07b032ee956b0c94552023-02-23T03:27:51ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172012-02-01301152010.17221/226/2010-CJFScjf-201201-0003Cyclodextrin production from amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans CCM 2012Marian Urban0Miloš Beran1Lubomír Adámek2Josef Drahorád3Petr Molík4Kristina Matušová5Department of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech RepublicDepartment of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech RepublicDepartment of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech RepublicDepartment of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech RepublicDepartment of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech RepublicAMR Amaranth, Blansko, Czech RepublicCyclodextrins (CDs) are synthesised by bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives. The production of α-, β-, and γ-CDs by CGTase from Paenibacillus mace-rans CCM 2012 was studied in regard to the effect of the starch source (amaranth, maize) on the yield of CDs. CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB under aerobic conditions. CGTase was partially purified by ammonium sulfate precipitation at 60% saturation. Electrophoretic analysis (SDS-PAGE) of the isolated CGTase enzyme was carried out according to the method by Laemmli (1970), the apparent molecular weight was in the range from 105 kDa to 114 kDa. All the commercially important α-, β-, and γ-CDs were detected chromatographically after the hydrolysis of the maize and amaranth (Amaranthus cruentus) starches with the isolated enzyme. The amaranth starch appears to be an excellent substrate for CDs production because of the high dispersibility, high starch-granule susceptibility to amylases, and the exceptionally high amylopectin content.https://cjfs.agriculturejournals.cz/artkey/cjf-201201-0003_cyclodextrin-production-from-amaranth-starch-by-cyclodextrin-glycosyltransferase-produced-by-paenibacillus-mace.phpamaranthus craentuscgtase enzymestarch substratestransglycosylation reactions |
| spellingShingle | Marian Urban Miloš Beran Lubomír Adámek Josef Drahorád Petr Molík Kristina Matušová Cyclodextrin production from amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans CCM 2012 Czech Journal of Food Sciences amaranthus craentus cgtase enzyme starch substrates transglycosylation reactions |
| title | Cyclodextrin production from amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans CCM 2012 |
| title_full | Cyclodextrin production from amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans CCM 2012 |
| title_fullStr | Cyclodextrin production from amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans CCM 2012 |
| title_full_unstemmed | Cyclodextrin production from amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans CCM 2012 |
| title_short | Cyclodextrin production from amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans CCM 2012 |
| title_sort | cyclodextrin production from amaranth starch by cyclodextrin glycosyltransferase produced by paenibacillus macerans ccm 2012 |
| topic | amaranthus craentus cgtase enzyme starch substrates transglycosylation reactions |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201201-0003_cyclodextrin-production-from-amaranth-starch-by-cyclodextrin-glycosyltransferase-produced-by-paenibacillus-mace.php |
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