Influence of <i>Serratia marcescens</i> and <i>Rhodococcus rhodnii</i> on the Humoral Immunity of <i>Rhodnius prolixus</i>
Chagas disease is a human infectious disease caused by <i>Trypanosoma cruzi</i> and can be transmitted by triatomine vectors, such as <i>Rhodnius prolixus</i>. One limiting factor for <i>T. cruzi</i> development is the composition of the bacterial gut microbiota i...
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/20/10901 |
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| author | Kate K. S. Batista Cecília S. Vieira Marcela B. Figueiredo Samara G. Costa-Latgé Patrícia Azambuja Fernando A. Genta Daniele P. Castro |
| author_facet | Kate K. S. Batista Cecília S. Vieira Marcela B. Figueiredo Samara G. Costa-Latgé Patrícia Azambuja Fernando A. Genta Daniele P. Castro |
| author_sort | Kate K. S. Batista |
| collection | DOAJ |
| description | Chagas disease is a human infectious disease caused by <i>Trypanosoma cruzi</i> and can be transmitted by triatomine vectors, such as <i>Rhodnius prolixus</i>. One limiting factor for <i>T. cruzi</i> development is the composition of the bacterial gut microbiota in the triatomine. Herein, we analyzed the humoral immune responses of <i>R. prolixus</i> nymphs treated with antibiotics and subsequently recolonized with either <i>Serratia marcescens</i> or <i>Rhodococcus rhodnii</i>. The treatment with antibiotics reduced the bacterial load in the digestive tract, and the recolonization with each bacterium was successfully detected seven days after treatment. The antibiotic-treated insects, recolonized with <i>S. marcescens</i>, presented reduced antibacterial activity against <i>Staphylococcus aureus</i> and phenoloxidase activity in hemolymph, and lower nitric oxide synthase (NOS) and higher defensin C gene (DefC) gene expression in the fat body. These insects also presented a higher expression of DefC, lower prolixicin (Prol), and lower NOS levels in the anterior midgut. However, the antibiotic-treated insects recolonized with <i>R. rhodnii</i> had increased antibacterial activity against <i>Escherichia coli</i> and lower activity against <i>S. aureus</i>, higher phenoloxidase activity in hemolymph, and lower NOS expression in the fat body. In the anterior midgut, these insects presented higher <i>NOS</i>, defensin A (DefA) and DefC expression, and lower Prol expression. The <i>R. prolixus</i> immune modulation by these two bacteria was observed not only in the midgut, but also systemically in the fat body, and may be crucial for the development and transmission of the parasites <i>Trypanosoma cruzi</i> and <i>Trypanosoma rangeli</i>. |
| first_indexed | 2024-03-10T06:31:03Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:31:03Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bdb0818e446c499fbf17723bc352f5a32023-11-22T18:30:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122201090110.3390/ijms222010901Influence of <i>Serratia marcescens</i> and <i>Rhodococcus rhodnii</i> on the Humoral Immunity of <i>Rhodnius prolixus</i>Kate K. S. Batista0Cecília S. Vieira1Marcela B. Figueiredo2Samara G. Costa-Latgé3Patrícia Azambuja4Fernando A. Genta5Daniele P. Castro6Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro 21040-360, BrazilLaboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro 21040-360, BrazilInstitute of Life Science, Swansea University Medical School, Swansea SA2 8PP, UKLaboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro 21040-360, BrazilPrograma de Pós-Graduação em Ciências e Biotecnologia, Universidade Federal Fluminense, Niteroi 24210-201, BrazilLaboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro 21040-360, BrazilLaboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro 21040-360, BrazilChagas disease is a human infectious disease caused by <i>Trypanosoma cruzi</i> and can be transmitted by triatomine vectors, such as <i>Rhodnius prolixus</i>. One limiting factor for <i>T. cruzi</i> development is the composition of the bacterial gut microbiota in the triatomine. Herein, we analyzed the humoral immune responses of <i>R. prolixus</i> nymphs treated with antibiotics and subsequently recolonized with either <i>Serratia marcescens</i> or <i>Rhodococcus rhodnii</i>. The treatment with antibiotics reduced the bacterial load in the digestive tract, and the recolonization with each bacterium was successfully detected seven days after treatment. The antibiotic-treated insects, recolonized with <i>S. marcescens</i>, presented reduced antibacterial activity against <i>Staphylococcus aureus</i> and phenoloxidase activity in hemolymph, and lower nitric oxide synthase (NOS) and higher defensin C gene (DefC) gene expression in the fat body. These insects also presented a higher expression of DefC, lower prolixicin (Prol), and lower NOS levels in the anterior midgut. However, the antibiotic-treated insects recolonized with <i>R. rhodnii</i> had increased antibacterial activity against <i>Escherichia coli</i> and lower activity against <i>S. aureus</i>, higher phenoloxidase activity in hemolymph, and lower NOS expression in the fat body. In the anterior midgut, these insects presented higher <i>NOS</i>, defensin A (DefA) and DefC expression, and lower Prol expression. The <i>R. prolixus</i> immune modulation by these two bacteria was observed not only in the midgut, but also systemically in the fat body, and may be crucial for the development and transmission of the parasites <i>Trypanosoma cruzi</i> and <i>Trypanosoma rangeli</i>.https://www.mdpi.com/1422-0067/22/20/10901insect immunitytriatominemicrobiotabacteriainsect physiologyantibiotic |
| spellingShingle | Kate K. S. Batista Cecília S. Vieira Marcela B. Figueiredo Samara G. Costa-Latgé Patrícia Azambuja Fernando A. Genta Daniele P. Castro Influence of <i>Serratia marcescens</i> and <i>Rhodococcus rhodnii</i> on the Humoral Immunity of <i>Rhodnius prolixus</i> International Journal of Molecular Sciences insect immunity triatomine microbiota bacteria insect physiology antibiotic |
| title | Influence of <i>Serratia marcescens</i> and <i>Rhodococcus rhodnii</i> on the Humoral Immunity of <i>Rhodnius prolixus</i> |
| title_full | Influence of <i>Serratia marcescens</i> and <i>Rhodococcus rhodnii</i> on the Humoral Immunity of <i>Rhodnius prolixus</i> |
| title_fullStr | Influence of <i>Serratia marcescens</i> and <i>Rhodococcus rhodnii</i> on the Humoral Immunity of <i>Rhodnius prolixus</i> |
| title_full_unstemmed | Influence of <i>Serratia marcescens</i> and <i>Rhodococcus rhodnii</i> on the Humoral Immunity of <i>Rhodnius prolixus</i> |
| title_short | Influence of <i>Serratia marcescens</i> and <i>Rhodococcus rhodnii</i> on the Humoral Immunity of <i>Rhodnius prolixus</i> |
| title_sort | influence of i serratia marcescens i and i rhodococcus rhodnii i on the humoral immunity of i rhodnius prolixus i |
| topic | insect immunity triatomine microbiota bacteria insect physiology antibiotic |
| url | https://www.mdpi.com/1422-0067/22/20/10901 |
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