Distribution of integrons and phylogenetic groups among highly virulent serotypes of Klebsiella pneumoniae in a Chinese tertiary hospital
Objectives: This study describes the distribution of integrons and phylogenetic groups among clinical highly virulent serotype (HVS) Klebsiella pneumoniae isolates in a Chinese tertiary hospital. Methods: Class 1, 2 and 3 integrases were identified by PCR in 90 clinical isolates of HVS K. pneumoniae...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716519303091 |
| _version_ | 1818914695219773440 |
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| author | Wenjian Liao Dan Li Fangpeng Liu Fang-ling Du Dan Long Wei Zhang Yang Liu |
| author_facet | Wenjian Liao Dan Li Fangpeng Liu Fang-ling Du Dan Long Wei Zhang Yang Liu |
| author_sort | Wenjian Liao |
| collection | DOAJ |
| description | Objectives: This study describes the distribution of integrons and phylogenetic groups among clinical highly virulent serotype (HVS) Klebsiella pneumoniae isolates in a Chinese tertiary hospital. Methods: Class 1, 2 and 3 integrases were identified by PCR in 90 clinical isolates of HVS K. pneumoniae. Antimicrobial susceptibility was determined by the disk diffusion method. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were used to analyse the genotypes of the HVS K. pneumoniae isolates. Results: Serotypes K1, K2, K20, K54 and K57 accounted for 54.5%, 21.1%, 1.1%, 18.9% and 4.4% of the 90 isolates. Among the 50 integron-positive isolates, 48 (96%) and 2 (4%) were classified as having class 1 (intI1) and class 2 (intI2) integrons, respectively. Gene cassettes encoding resistance to trimethoprim (dfr) and aminoglycosides (aac, aad) were found to be predominant in class 1 integrons. In addition, the most prevalent sequence type (ST) among the HVS K. pneumoniae isolates was ST23 (49/90; 54.4%), followed by ST29 (11/90; 12.2%), ST86 (10/90; 11.1%), ST65 (9/90; 10.0%), ST15 (6/90; 6.7%), ST412 (4/90; 4.4%) and ST34 (1/90; 1.1%). Conclusion: In summary, a high prevalence of integrons (55.6%) was found among HVS K. pneumoniae isolates in a Chinese tertiary hospital. Class 1 integrons were the most predominant and their variable regions were polymorphic. The presence of integrons in HVS K. pneumoniae isolates results in increased antimicrobial resistance. |
| first_indexed | 2024-12-19T23:50:28Z |
| format | Article |
| id | doaj.art-bdb0a956a3b64d7b82f29257976bc60b |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-19T23:50:28Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-bdb0a956a3b64d7b82f29257976bc60b2022-12-21T20:01:09ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-06-0121278284Distribution of integrons and phylogenetic groups among highly virulent serotypes of Klebsiella pneumoniae in a Chinese tertiary hospitalWenjian Liao0Dan Li1Fangpeng Liu2Fang-ling Du3Dan Long4Wei Zhang5Yang Liu6Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, ChinaDepartment of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, ChinaDepartment of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, ChinaDepartment of Clinical Microbiology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, ChinaDepartment of Clinical Microbiology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, ChinaDepartment of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, ChinaDepartment of Clinical Microbiology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China; Corresponding author.Objectives: This study describes the distribution of integrons and phylogenetic groups among clinical highly virulent serotype (HVS) Klebsiella pneumoniae isolates in a Chinese tertiary hospital. Methods: Class 1, 2 and 3 integrases were identified by PCR in 90 clinical isolates of HVS K. pneumoniae. Antimicrobial susceptibility was determined by the disk diffusion method. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were used to analyse the genotypes of the HVS K. pneumoniae isolates. Results: Serotypes K1, K2, K20, K54 and K57 accounted for 54.5%, 21.1%, 1.1%, 18.9% and 4.4% of the 90 isolates. Among the 50 integron-positive isolates, 48 (96%) and 2 (4%) were classified as having class 1 (intI1) and class 2 (intI2) integrons, respectively. Gene cassettes encoding resistance to trimethoprim (dfr) and aminoglycosides (aac, aad) were found to be predominant in class 1 integrons. In addition, the most prevalent sequence type (ST) among the HVS K. pneumoniae isolates was ST23 (49/90; 54.4%), followed by ST29 (11/90; 12.2%), ST86 (10/90; 11.1%), ST65 (9/90; 10.0%), ST15 (6/90; 6.7%), ST412 (4/90; 4.4%) and ST34 (1/90; 1.1%). Conclusion: In summary, a high prevalence of integrons (55.6%) was found among HVS K. pneumoniae isolates in a Chinese tertiary hospital. Class 1 integrons were the most predominant and their variable regions were polymorphic. The presence of integrons in HVS K. pneumoniae isolates results in increased antimicrobial resistance.http://www.sciencedirect.com/science/article/pii/S2213716519303091IntegronKlebsiella pneumoniaeHighly virulent serotypeDrug resistance |
| spellingShingle | Wenjian Liao Dan Li Fangpeng Liu Fang-ling Du Dan Long Wei Zhang Yang Liu Distribution of integrons and phylogenetic groups among highly virulent serotypes of Klebsiella pneumoniae in a Chinese tertiary hospital Journal of Global Antimicrobial Resistance Integron Klebsiella pneumoniae Highly virulent serotype Drug resistance |
| title | Distribution of integrons and phylogenetic groups among highly virulent serotypes of Klebsiella pneumoniae in a Chinese tertiary hospital |
| title_full | Distribution of integrons and phylogenetic groups among highly virulent serotypes of Klebsiella pneumoniae in a Chinese tertiary hospital |
| title_fullStr | Distribution of integrons and phylogenetic groups among highly virulent serotypes of Klebsiella pneumoniae in a Chinese tertiary hospital |
| title_full_unstemmed | Distribution of integrons and phylogenetic groups among highly virulent serotypes of Klebsiella pneumoniae in a Chinese tertiary hospital |
| title_short | Distribution of integrons and phylogenetic groups among highly virulent serotypes of Klebsiella pneumoniae in a Chinese tertiary hospital |
| title_sort | distribution of integrons and phylogenetic groups among highly virulent serotypes of klebsiella pneumoniae in a chinese tertiary hospital |
| topic | Integron Klebsiella pneumoniae Highly virulent serotype Drug resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2213716519303091 |
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