Selection of Yeasts as Starter Cultures for Table Olives: a Step-by-Step Procedure

In the past yeasts were traditionally regarded as the spoiling microorganisms for table olives; however, their role and impact for product quality and for the correct course of fermentation has been revised and nowadays many authors suggest a controlled inoculum of yeasts both in alkali-treated and...

Full description

Bibliographic Details
Main Authors: Antonio eBevilacqua, Maria Rosaria eCorbo, Milena eSinigaglia
Format: Article
Language:English
Published: Frontiers Media S.A. 2012-05-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2012.00194/full
_version_ 1818482903434133504
author Antonio eBevilacqua
Maria Rosaria eCorbo
Milena eSinigaglia
author_facet Antonio eBevilacqua
Maria Rosaria eCorbo
Milena eSinigaglia
author_sort Antonio eBevilacqua
collection DOAJ
description In the past yeasts were traditionally regarded as the spoiling microorganisms for table olives; however, their role and impact for product quality and for the correct course of fermentation has been revised and nowadays many authors suggest a controlled inoculum of yeasts both in alkali-treated and untreated olives.The selection of a starter is a complex process, involving different steps. After strain isolation from raw material, the first step is the identification through phenotyping and genotypic methods; then, isolates should be characterized to assess their GRAS (generally recognized as safe) status and technological properties (growth with salt added, at various temperatures and pHs, pectolytic and xylanolytic activity, lipolytic activity, resistance to some preservatives, functional impact).After studying these properties, the results can be submitted to data analysis (many times a statistical multivariate approach) and strain selection. The number of strains to be selected depends on several factors, above all on the main goal: obtaining a single or a multiple-strain starter.After this step, starter should be used for a pilot fermentation on a lab scale, highlighting its performances, limits and benefits, as well as all the issues related to its production, storage and stability throughout the time. Finally, starter optimization conducted on a lab scale should be verified on real conditions.
first_indexed 2024-12-10T11:52:52Z
format Article
id doaj.art-bdb596b3b40d462190cc729f7c1ddeb4
institution Directory Open Access Journal
issn 1664-302X
language English
last_indexed 2024-12-10T11:52:52Z
publishDate 2012-05-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Microbiology
spelling doaj.art-bdb596b3b40d462190cc729f7c1ddeb42022-12-22T01:49:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2012-05-01310.3389/fmicb.2012.0019427312Selection of Yeasts as Starter Cultures for Table Olives: a Step-by-Step ProcedureAntonio eBevilacqua0Maria Rosaria eCorbo1Milena eSinigaglia2University of FoggiaUniversity of FoggiaUniversity of FoggiaIn the past yeasts were traditionally regarded as the spoiling microorganisms for table olives; however, their role and impact for product quality and for the correct course of fermentation has been revised and nowadays many authors suggest a controlled inoculum of yeasts both in alkali-treated and untreated olives.The selection of a starter is a complex process, involving different steps. After strain isolation from raw material, the first step is the identification through phenotyping and genotypic methods; then, isolates should be characterized to assess their GRAS (generally recognized as safe) status and technological properties (growth with salt added, at various temperatures and pHs, pectolytic and xylanolytic activity, lipolytic activity, resistance to some preservatives, functional impact).After studying these properties, the results can be submitted to data analysis (many times a statistical multivariate approach) and strain selection. The number of strains to be selected depends on several factors, above all on the main goal: obtaining a single or a multiple-strain starter.After this step, starter should be used for a pilot fermentation on a lab scale, highlighting its performances, limits and benefits, as well as all the issues related to its production, storage and stability throughout the time. Finally, starter optimization conducted on a lab scale should be verified on real conditions.http://journal.frontiersin.org/Journal/10.3389/fmicb.2012.00194/fullYeastsTable OlivesStarter SelectionValidation
spellingShingle Antonio eBevilacqua
Maria Rosaria eCorbo
Milena eSinigaglia
Selection of Yeasts as Starter Cultures for Table Olives: a Step-by-Step Procedure
Frontiers in Microbiology
Yeasts
Table Olives
Starter Selection
Validation
title Selection of Yeasts as Starter Cultures for Table Olives: a Step-by-Step Procedure
title_full Selection of Yeasts as Starter Cultures for Table Olives: a Step-by-Step Procedure
title_fullStr Selection of Yeasts as Starter Cultures for Table Olives: a Step-by-Step Procedure
title_full_unstemmed Selection of Yeasts as Starter Cultures for Table Olives: a Step-by-Step Procedure
title_short Selection of Yeasts as Starter Cultures for Table Olives: a Step-by-Step Procedure
title_sort selection of yeasts as starter cultures for table olives a step by step procedure
topic Yeasts
Table Olives
Starter Selection
Validation
url http://journal.frontiersin.org/Journal/10.3389/fmicb.2012.00194/full
work_keys_str_mv AT antonioebevilacqua selectionofyeastsasstarterculturesfortableolivesastepbystepprocedure
AT mariarosariaecorbo selectionofyeastsasstarterculturesfortableolivesastepbystepprocedure
AT milenaesinigaglia selectionofyeastsasstarterculturesfortableolivesastepbystepprocedure