CCR6+ Th cell distribution differentiates systemic lupus erythematosus patients based on anti-dsDNA antibody status
Background Systemic lupus erythematosus (SLE) disease has been shown to be associated with the generation of multiple auto-antibodies. Among these, anti-dsDNA antibodies (anti-DNAs) are specific and play a pathogenic role in SLE. Indeed, anti-DNA+ SLE patients display a worse disease course. The gen...
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PeerJ Inc.
2018-02-01
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| Online Access: | https://peerj.com/articles/4294.pdf |
| _version_ | 1797425630351982592 |
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| author | Wei Zhong Zhenyu Jiang Jiang Wu Yanfang Jiang Ling Zhao |
| author_facet | Wei Zhong Zhenyu Jiang Jiang Wu Yanfang Jiang Ling Zhao |
| author_sort | Wei Zhong |
| collection | DOAJ |
| description | Background Systemic lupus erythematosus (SLE) disease has been shown to be associated with the generation of multiple auto-antibodies. Among these, anti-dsDNA antibodies (anti-DNAs) are specific and play a pathogenic role in SLE. Indeed, anti-DNA+ SLE patients display a worse disease course. The generation of these pathogenic anti-DNAs has been attributed to the interaction between aberrant T helper (Th) cells and autoimmune B cells. Thus, in this study we have investigated whether CCR6+Th cells have the ability to differentiate SLE patients based on anti-DNA status, and if their distribution has any correlation with disease activity. Methods We recruited 25 anti-DNA+ and 25 anti-DNA− treatment-naive onset SLE patients, matched for various clinical characteristics in our nested matched case-control study. CCR6+ Th cells and their additional subsets were analyzed in each patient by flow cytometry. Results Anti-DNA+ SLE patients specifically had a higher percentage of Th cells expressing CCR6 and CXCR3. Further analysis of CCR6+ Th cell subsets showed that anti-DNA+ SLE patients had elevated proportions of Th9, Th17, Th17.1 and CCR4/CXCR3 double-negative (DN) cells. However, the proportions of CCR6− Th subsets, including Th1 and Th2 cells, did not show any association with anti-DNA status. Finally, we identified a correlation between CCR6+ Th subsets and clinical indicators, specifically in anti-DNA+ SLE patients. Conclusions Our data indicated that CCR6+ Th cells and their subsets were elevated and correlated with disease activity in anti-DNA+ SLE patients. We speculated that CCR6+ Th cells may contribute to distinct disease severity in anti-DNA+ SLE patients. |
| first_indexed | 2024-03-09T08:18:52Z |
| format | Article |
| id | doaj.art-bdb6d89dd12c47bc84684d21f457385f |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:18:52Z |
| publishDate | 2018-02-01 |
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| spelling | doaj.art-bdb6d89dd12c47bc84684d21f457385f2023-12-02T21:54:23ZengPeerJ Inc.PeerJ2167-83592018-02-016e429410.7717/peerj.4294CCR6+ Th cell distribution differentiates systemic lupus erythematosus patients based on anti-dsDNA antibody statusWei Zhong0Zhenyu Jiang1Jiang Wu2Yanfang Jiang3Ling Zhao4Department of Rheumatology, First Hospital, Jilin University, Changchun, ChinaDepartment of Rheumatology, First Hospital, Jilin University, Changchun, ChinaCollege of Electrical Engineering and Instrumentation, Jilin University, Changchun, ChinaGenetic Diagnosis Center, First Hospital, Jilin University, Changchun, ChinaDepartment of Rheumatology, First Hospital, Jilin University, Changchun, ChinaBackground Systemic lupus erythematosus (SLE) disease has been shown to be associated with the generation of multiple auto-antibodies. Among these, anti-dsDNA antibodies (anti-DNAs) are specific and play a pathogenic role in SLE. Indeed, anti-DNA+ SLE patients display a worse disease course. The generation of these pathogenic anti-DNAs has been attributed to the interaction between aberrant T helper (Th) cells and autoimmune B cells. Thus, in this study we have investigated whether CCR6+Th cells have the ability to differentiate SLE patients based on anti-DNA status, and if their distribution has any correlation with disease activity. Methods We recruited 25 anti-DNA+ and 25 anti-DNA− treatment-naive onset SLE patients, matched for various clinical characteristics in our nested matched case-control study. CCR6+ Th cells and their additional subsets were analyzed in each patient by flow cytometry. Results Anti-DNA+ SLE patients specifically had a higher percentage of Th cells expressing CCR6 and CXCR3. Further analysis of CCR6+ Th cell subsets showed that anti-DNA+ SLE patients had elevated proportions of Th9, Th17, Th17.1 and CCR4/CXCR3 double-negative (DN) cells. However, the proportions of CCR6− Th subsets, including Th1 and Th2 cells, did not show any association with anti-DNA status. Finally, we identified a correlation between CCR6+ Th subsets and clinical indicators, specifically in anti-DNA+ SLE patients. Conclusions Our data indicated that CCR6+ Th cells and their subsets were elevated and correlated with disease activity in anti-DNA+ SLE patients. We speculated that CCR6+ Th cells may contribute to distinct disease severity in anti-DNA+ SLE patients.https://peerj.com/articles/4294.pdfSystemic lupus erythematosusCCR6+ T helper cellsAnti-dsDNA antibody |
| spellingShingle | Wei Zhong Zhenyu Jiang Jiang Wu Yanfang Jiang Ling Zhao CCR6+ Th cell distribution differentiates systemic lupus erythematosus patients based on anti-dsDNA antibody status PeerJ Systemic lupus erythematosus CCR6+ T helper cells Anti-dsDNA antibody |
| title | CCR6+ Th cell distribution differentiates systemic lupus erythematosus patients based on anti-dsDNA antibody status |
| title_full | CCR6+ Th cell distribution differentiates systemic lupus erythematosus patients based on anti-dsDNA antibody status |
| title_fullStr | CCR6+ Th cell distribution differentiates systemic lupus erythematosus patients based on anti-dsDNA antibody status |
| title_full_unstemmed | CCR6+ Th cell distribution differentiates systemic lupus erythematosus patients based on anti-dsDNA antibody status |
| title_short | CCR6+ Th cell distribution differentiates systemic lupus erythematosus patients based on anti-dsDNA antibody status |
| title_sort | ccr6 th cell distribution differentiates systemic lupus erythematosus patients based on anti dsdna antibody status |
| topic | Systemic lupus erythematosus CCR6+ T helper cells Anti-dsDNA antibody |
| url | https://peerj.com/articles/4294.pdf |
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