Strategies for Improving Enzymes for Efficient Biocatalysis
Biocatalytic processes are finding increasingly widespread application not only in academia, but also in industry. This is particularly true in the pharmaceutical and agrochemical industries where the need for optically pure molecules is critical. Biocatalysis is also receiving a major thrust from t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2004-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/163381 |
| _version_ | 1797422870926721024 |
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| author | Marek Adamczak Sajja Hari Krishna |
| author_facet | Marek Adamczak Sajja Hari Krishna |
| author_sort | Marek Adamczak |
| collection | DOAJ |
| description | Biocatalytic processes are finding increasingly widespread application not only in academia, but also in industry. This is particularly true in the pharmaceutical and agrochemical industries where the need for optically pure molecules is critical. Biocatalysis is also receiving a major thrust from the generation of new and novel biocatalysts via microbial screening, developments in the biocatalytic processes themselves (e.g. use of nonaqueous solvents for synthetic purposes) and improvements in the activation of the enzymes by numerous mechanisms (solvent and enzyme modifications), including protein engineering. The most dramatic results have been achieved by targeting the gene encoding a particular enzyme in order to undertake protein engineering by rational and non-rational methods. This review discusses various means of improving enzyme properties or creating new activities. |
| first_indexed | 2024-03-09T07:39:41Z |
| format | Article |
| id | doaj.art-bdb7839f408846019087d1f291cacf63 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T07:39:41Z |
| publishDate | 2004-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-bdb7839f408846019087d1f291cacf632023-12-03T05:04:33ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062004-01-01424251264Strategies for Improving Enzymes for Efficient BiocatalysisMarek Adamczak0Sajja Hari Krishna1Chair of Food Biotechnology, University of Warmia and Mazury in Olsztyn, PL-10 718 Olsztyn, PolandDepartment of Chemistry and Center for Fundamental and Applied Molecular Evolution, Emory University, Atlanta, GA-30 322, USABiocatalytic processes are finding increasingly widespread application not only in academia, but also in industry. This is particularly true in the pharmaceutical and agrochemical industries where the need for optically pure molecules is critical. Biocatalysis is also receiving a major thrust from the generation of new and novel biocatalysts via microbial screening, developments in the biocatalytic processes themselves (e.g. use of nonaqueous solvents for synthetic purposes) and improvements in the activation of the enzymes by numerous mechanisms (solvent and enzyme modifications), including protein engineering. The most dramatic results have been achieved by targeting the gene encoding a particular enzyme in order to undertake protein engineering by rational and non-rational methods. This review discusses various means of improving enzyme properties or creating new activities.http://hrcak.srce.hr/file/163381biocatalysisbiocatalyst engineeringdirected evolutionenzymemedium engineering |
| spellingShingle | Marek Adamczak Sajja Hari Krishna Strategies for Improving Enzymes for Efficient Biocatalysis Food Technology and Biotechnology biocatalysis biocatalyst engineering directed evolution enzyme medium engineering |
| title | Strategies for Improving Enzymes for Efficient Biocatalysis |
| title_full | Strategies for Improving Enzymes for Efficient Biocatalysis |
| title_fullStr | Strategies for Improving Enzymes for Efficient Biocatalysis |
| title_full_unstemmed | Strategies for Improving Enzymes for Efficient Biocatalysis |
| title_short | Strategies for Improving Enzymes for Efficient Biocatalysis |
| title_sort | strategies for improving enzymes for efficient biocatalysis |
| topic | biocatalysis biocatalyst engineering directed evolution enzyme medium engineering |
| url | http://hrcak.srce.hr/file/163381 |
| work_keys_str_mv | AT marekadamczak strategiesforimprovingenzymesforefficientbiocatalysis AT sajjaharikrishna strategiesforimprovingenzymesforefficientbiocatalysis |