Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease
Introduction: The inflammatory process in Crohn’s disease (CD) is closely associated with the formation of reactive oxygen species. Antioxidant enzymes can play an important role in the outcome of CD and may influence postoperative recurrence in these patients. The aim of our study was to evaluate g...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Karolinum Press
2021-11-01
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| Series: | Acta Medica |
| Online Access: | https://actamedica.lfhk.cuni.cz/64/3/0153/ |
| _version_ | 1818932717455147008 |
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| author | Otakar Sotona Eva Peterová Július Örhalmi Tomáš Dušek Alena Mrkvicová Veronika Knoblochová Petr Lochman Ondřej Malý Jiří Páral Jan Bureš |
| author_facet | Otakar Sotona Eva Peterová Július Örhalmi Tomáš Dušek Alena Mrkvicová Veronika Knoblochová Petr Lochman Ondřej Malý Jiří Páral Jan Bureš |
| author_sort | Otakar Sotona |
| collection | DOAJ |
| description | Introduction: The inflammatory process in Crohn’s disease (CD) is closely associated with the formation of reactive oxygen species. Antioxidant enzymes can play an important role in the outcome of CD and may influence postoperative recurrence in these patients. The aim of our study was to evaluate gene expression of intracellular antioxidant enzymes in surgically resected intestinal specimens of patients with CD, both in macroscopically normal and in inflamed tissue. Methods: A total of 28 patients referred for elective bowel resection were enrolled in the study. Full-thickness small intestinal specimens were investigated. Gene expression of antioxidant enzymes – superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GSR) – was evaluated both in macroscopically normal and inflamed samples. Results: There were significantly lower levels of SOD1 mRNA (p = 0.007) and GSR mRNA (p = 0.027) in inflamed tissue compared to macroscopically normal areas. No significant differences were found between affected and non-affected intestinal segments in mRNA for SOD2, SOD3 and GPX. Conclusions: Our pilot data clearly showed that the gene expression of major antioxidant enzymes is not a uniform mechanism in the pathogenesis of Crohn’s disease. Topically decreased gene expression of SOD1 and GSR might facilitate the segmental tissue injury caused by reactive oxygen species. |
| first_indexed | 2024-12-20T04:36:55Z |
| format | Article |
| id | doaj.art-bdb803adbf96474e884589ea20ed3520 |
| institution | Directory Open Access Journal |
| issn | 1211-4286 1805-9694 |
| language | English |
| last_indexed | 2024-12-20T04:36:55Z |
| publishDate | 2021-11-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Acta Medica |
| spelling | doaj.art-bdb803adbf96474e884589ea20ed35202022-12-21T19:53:14ZengKarolinum PressActa Medica1211-42861805-96942021-11-0164315315710.14712/18059694.2021.26Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s DiseaseOtakar SotonaEva Peterováhttps://orcid.org/0000-0001-7866-9722Július ÖrhalmiTomáš DušekAlena MrkvicováVeronika KnoblochováPetr LochmanOndřej MalýJiří PáralJan Burešhttps://orcid.org/0000-0003-0326-117XIntroduction: The inflammatory process in Crohn’s disease (CD) is closely associated with the formation of reactive oxygen species. Antioxidant enzymes can play an important role in the outcome of CD and may influence postoperative recurrence in these patients. The aim of our study was to evaluate gene expression of intracellular antioxidant enzymes in surgically resected intestinal specimens of patients with CD, both in macroscopically normal and in inflamed tissue. Methods: A total of 28 patients referred for elective bowel resection were enrolled in the study. Full-thickness small intestinal specimens were investigated. Gene expression of antioxidant enzymes – superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GSR) – was evaluated both in macroscopically normal and inflamed samples. Results: There were significantly lower levels of SOD1 mRNA (p = 0.007) and GSR mRNA (p = 0.027) in inflamed tissue compared to macroscopically normal areas. No significant differences were found between affected and non-affected intestinal segments in mRNA for SOD2, SOD3 and GPX. Conclusions: Our pilot data clearly showed that the gene expression of major antioxidant enzymes is not a uniform mechanism in the pathogenesis of Crohn’s disease. Topically decreased gene expression of SOD1 and GSR might facilitate the segmental tissue injury caused by reactive oxygen species.https://actamedica.lfhk.cuni.cz/64/3/0153/ |
| spellingShingle | Otakar Sotona Eva Peterová Július Örhalmi Tomáš Dušek Alena Mrkvicová Veronika Knoblochová Petr Lochman Ondřej Malý Jiří Páral Jan Bureš Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease Acta Medica |
| title | Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease |
| title_full | Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease |
| title_fullStr | Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease |
| title_full_unstemmed | Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease |
| title_short | Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease |
| title_sort | gene expression of antioxidant enzymes in the resected intestine in crohn s disease |
| url | https://actamedica.lfhk.cuni.cz/64/3/0153/ |
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