Sample preparation and analysis of proficiency test containing Staphylococcus aureus with drug matrix
ObjectiveTo prepare samples of proficiency testing （PT） containing live Staphylococcus aureus with drug matrix in microbial PT program of drug testing， and to evaluate the laboratory capability of microbiology tests in China.MethodsTwo kinds of PT samples containing living bacteria with drug matrix...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Shanghai Preventive Medicine Association
2022-03-01
|
| Series: | Shanghai yufang yixue |
| Subjects: | |
| Online Access: | http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.21238 |
| _version_ | 1797939258575552512 |
|---|---|
| author | FAN Yiling LI Fang YANG Yan QIN Feng LIU Hao YANG Meicheng |
| author_facet | FAN Yiling LI Fang YANG Yan QIN Feng LIU Hao YANG Meicheng |
| author_sort | FAN Yiling |
| collection | DOAJ |
| description | ObjectiveTo prepare samples of proficiency testing （PT） containing live Staphylococcus aureus with drug matrix in microbial PT program of drug testing， and to evaluate the laboratory capability of microbiology tests in China.MethodsTwo kinds of PT samples containing living bacteria with drug matrix were prepared and evaluated. The results of laboratories participated in PT program and their response to the survey questionnaire were collected and analyzed.ResultsThe homogeneity and stability of the PT samples complied with the requirements of CNAS-CL03： 2010. Samples could be stored stably for at least 6 months at -20 ℃. Among 63 laboratories participating in PT program， 53 laboratories （84.1%） achieved satisfactory results. The satisfaction rate was 94.1% （16/17） in 17 government laboratories （27.0% of total 63 laboratories）， 81.4% （35/43） in 43 pharmaceutical quality control laboratories （68.3% of total 63 laboratories）， and 66.7% （2/3） in 3 non-government laboratories （4.8% of total 63 laboratories）， respectively.ConclusionThe government laboratories performed better than pharmaceutical quality control laboratories in microbiology tests of drug， and the testing abilities of some pharmaceutical laboratories needs to be improved. The preparation and application of microbial samples in drug matrix could provide evaluation tools for drug testing laboratories in microbiology. |
| first_indexed | 2024-04-10T19:12:33Z |
| format | Article |
| id | doaj.art-bdbc2354bf304639933955a59fa6de77 |
| institution | Directory Open Access Journal |
| issn | 1004-9231 |
| language | zho |
| last_indexed | 2024-04-10T19:12:33Z |
| publishDate | 2022-03-01 |
| publisher | Shanghai Preventive Medicine Association |
| record_format | Article |
| series | Shanghai yufang yixue |
| spelling | doaj.art-bdbc2354bf304639933955a59fa6de772023-01-30T10:52:56ZzhoShanghai Preventive Medicine AssociationShanghai yufang yixue1004-92312022-03-0134327928210.19428/j.cnki.sjpm.2022.212381004-9231（2022）03-0279-04Sample preparation and analysis of proficiency test containing Staphylococcus aureus with drug matrixFAN YilingLI FangYANG YanQIN FengLIU HaoYANG MeichengObjectiveTo prepare samples of proficiency testing （PT） containing live Staphylococcus aureus with drug matrix in microbial PT program of drug testing， and to evaluate the laboratory capability of microbiology tests in China.MethodsTwo kinds of PT samples containing living bacteria with drug matrix were prepared and evaluated. The results of laboratories participated in PT program and their response to the survey questionnaire were collected and analyzed.ResultsThe homogeneity and stability of the PT samples complied with the requirements of CNAS-CL03： 2010. Samples could be stored stably for at least 6 months at -20 ℃. Among 63 laboratories participating in PT program， 53 laboratories （84.1%） achieved satisfactory results. The satisfaction rate was 94.1% （16/17） in 17 government laboratories （27.0% of total 63 laboratories）， 81.4% （35/43） in 43 pharmaceutical quality control laboratories （68.3% of total 63 laboratories）， and 66.7% （2/3） in 3 non-government laboratories （4.8% of total 63 laboratories）， respectively.ConclusionThe government laboratories performed better than pharmaceutical quality control laboratories in microbiology tests of drug， and the testing abilities of some pharmaceutical laboratories needs to be improved. The preparation and application of microbial samples in drug matrix could provide evaluation tools for drug testing laboratories in microbiology.http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.21238drug matrixstaphylococcus aureusproficiency testhomogeneitystability |
| spellingShingle | FAN Yiling LI Fang YANG Yan QIN Feng LIU Hao YANG Meicheng Sample preparation and analysis of proficiency test containing Staphylococcus aureus with drug matrix Shanghai yufang yixue drug matrix staphylococcus aureus proficiency test homogeneity stability |
| title | Sample preparation and analysis of proficiency test containing Staphylococcus aureus with drug matrix |
| title_full | Sample preparation and analysis of proficiency test containing Staphylococcus aureus with drug matrix |
| title_fullStr | Sample preparation and analysis of proficiency test containing Staphylococcus aureus with drug matrix |
| title_full_unstemmed | Sample preparation and analysis of proficiency test containing Staphylococcus aureus with drug matrix |
| title_short | Sample preparation and analysis of proficiency test containing Staphylococcus aureus with drug matrix |
| title_sort | sample preparation and analysis of proficiency test containing staphylococcus aureus with drug matrix |
| topic | drug matrix staphylococcus aureus proficiency test homogeneity stability |
| url | http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.21238 |
| work_keys_str_mv | AT fanyiling samplepreparationandanalysisofproficiencytestcontainingstaphylococcusaureuswithdrugmatrix AT lifang samplepreparationandanalysisofproficiencytestcontainingstaphylococcusaureuswithdrugmatrix AT yangyan samplepreparationandanalysisofproficiencytestcontainingstaphylococcusaureuswithdrugmatrix AT qinfeng samplepreparationandanalysisofproficiencytestcontainingstaphylococcusaureuswithdrugmatrix AT liuhao samplepreparationandanalysisofproficiencytestcontainingstaphylococcusaureuswithdrugmatrix AT yangmeicheng samplepreparationandanalysisofproficiencytestcontainingstaphylococcusaureuswithdrugmatrix |