Bacterial Communities in Rhizosphere of Maize Studied by T-RFLP
The terminal restriction fragment length polymorphism munities from different collecting places was evaluated was used to determine the bacterial diversity in rhizo- by principal component analysis. Results showed that sphere of maize (Zea mays L.) collected from four sites the most different bacter...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2014-12-01
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| Series: | Agriculture |
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| Online Access: | https://doi.org/10.2478/agri-2014-0011 |
| _version_ | 1819109071095070720 |
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| author | Ondreičková Katarína Ficek Andrej Mihálik Daniel Gubišová Marcela Hudcovicová Martina Drahovská Hana Kraic Ján |
| author_facet | Ondreičková Katarína Ficek Andrej Mihálik Daniel Gubišová Marcela Hudcovicová Martina Drahovská Hana Kraic Ján |
| author_sort | Ondreičková Katarína |
| collection | DOAJ |
| description | The terminal restriction fragment length polymorphism munities from different collecting places was evaluated was used to determine the bacterial diversity in rhizo- by principal component analysis. Results showed that sphere of maize (Zea mays L.) collected from four sites the most different bacterial community originated from of experimental field plot in two dates of the vegetation marginal part of the experimental field plot collected in season (July and September). The 16S rRNA gene was September was caused probably by combination of the amplified from metagenomic DNA using universal eubac- marginal effect and drought before sampling date in Sep- terial primers and PCR products were digested separately tember. Other rhizosphere samples showed from moderate with three restriction enzymes. Significant differences in to small differences in the structure of the bacterial com- the number of terminal restriction fragments among rhi- munity. Nevertheless, significant differences among all zosphere samples and between sampling dates were not collected bacterial communities were not observed. detected (P < 0.05). Variation within the bacterial communities from different collecting places was evaluated by principal component analysis. Results showed that the most different bacterial community originated from marginal part of the experimental field plot collected in September was caused probably by combination of the marginal effect and drought before sampling date in September. Other rhizosphere samples showed from moderate to small differences in the structure of the bacterial community. Nevertheless, significant differences among all collected bacterial communities were not observed. |
| first_indexed | 2024-12-22T03:19:59Z |
| format | Article |
| id | doaj.art-bdbd32256ca4479f811c728324c12ef2 |
| institution | Directory Open Access Journal |
| issn | 1338-4376 |
| language | English |
| last_indexed | 2024-12-22T03:19:59Z |
| publishDate | 2014-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-bdbd32256ca4479f811c728324c12ef22022-12-21T18:40:45ZengSciendoAgriculture1338-43762014-12-016039810410.2478/agri-2014-0011agri-2014-0011Bacterial Communities in Rhizosphere of Maize Studied by T-RFLPOndreičková Katarína0Ficek Andrej1Mihálik Daniel2Gubišová Marcela3Hudcovicová Martina4Drahovská Hana5Kraic Ján6 National Agricultural and Food CentreComenius University in Bratislava National Agricultural and Food Centre National Agricultural and Food Centre National Agricultural and Food CentreComenius University in Bratislava National Agricultural and Food CentreThe terminal restriction fragment length polymorphism munities from different collecting places was evaluated was used to determine the bacterial diversity in rhizo- by principal component analysis. Results showed that sphere of maize (Zea mays L.) collected from four sites the most different bacterial community originated from of experimental field plot in two dates of the vegetation marginal part of the experimental field plot collected in season (July and September). The 16S rRNA gene was September was caused probably by combination of the amplified from metagenomic DNA using universal eubac- marginal effect and drought before sampling date in Sep- terial primers and PCR products were digested separately tember. Other rhizosphere samples showed from moderate with three restriction enzymes. Significant differences in to small differences in the structure of the bacterial com- the number of terminal restriction fragments among rhi- munity. Nevertheless, significant differences among all zosphere samples and between sampling dates were not collected bacterial communities were not observed. detected (P < 0.05). Variation within the bacterial communities from different collecting places was evaluated by principal component analysis. Results showed that the most different bacterial community originated from marginal part of the experimental field plot collected in September was caused probably by combination of the marginal effect and drought before sampling date in September. Other rhizosphere samples showed from moderate to small differences in the structure of the bacterial community. Nevertheless, significant differences among all collected bacterial communities were not observed.https://doi.org/10.2478/agri-2014-0011bacterial diversitymaizerhizosphereterminal restriction fragment16s rdna |
| spellingShingle | Ondreičková Katarína Ficek Andrej Mihálik Daniel Gubišová Marcela Hudcovicová Martina Drahovská Hana Kraic Ján Bacterial Communities in Rhizosphere of Maize Studied by T-RFLP Agriculture bacterial diversity maize rhizosphere terminal restriction fragment 16s rdna |
| title | Bacterial Communities in Rhizosphere of Maize Studied by T-RFLP |
| title_full | Bacterial Communities in Rhizosphere of Maize Studied by T-RFLP |
| title_fullStr | Bacterial Communities in Rhizosphere of Maize Studied by T-RFLP |
| title_full_unstemmed | Bacterial Communities in Rhizosphere of Maize Studied by T-RFLP |
| title_short | Bacterial Communities in Rhizosphere of Maize Studied by T-RFLP |
| title_sort | bacterial communities in rhizosphere of maize studied by t rflp |
| topic | bacterial diversity maize rhizosphere terminal restriction fragment 16s rdna |
| url | https://doi.org/10.2478/agri-2014-0011 |
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