Development of Phylogenetic Markers for <i>Sebacina</i> (Sebacinaceae) Mycorrhizal Fungi Associated with Australian Orchids
Premise of the study: To investigate fungal species identity and diversity in mycorrhizal fungi of order Sebacinales, we developed phylogenetic markers. These new markers will enable future studies investigating species delineation and phylogenetic relationships of the fungal symbionts and facilitat...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-06-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1400015 |
| _version_ | 1819111904608518144 |
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| author | Monica P. Ruibal Rod Peakall Sylvain Foret Celeste C. Linde |
| author_facet | Monica P. Ruibal Rod Peakall Sylvain Foret Celeste C. Linde |
| author_sort | Monica P. Ruibal |
| collection | DOAJ |
| description | Premise of the study: To investigate fungal species identity and diversity in mycorrhizal fungi of order Sebacinales, we developed phylogenetic markers. These new markers will enable future studies investigating species delineation and phylogenetic relationships of the fungal symbionts and facilitate investigations into evolutionary interactions among <i>Sebacina</i> species and their orchid hosts.
Methods and Results: We generated partial genome sequences for a <i>Sebacina</i> symbiont originating from <i>Caladenia huegelii</i> with 454 genome sequencing and from three symbionts from <i>Eriochilus dilatatus</i> and one from <i>E. pulchellus</i> using Illumina sequencing. Six nuclear and two mitochondrial loci showed high variability (10–31% parsimony informative sites) for Sebacinales mycorrhizal fungi across four genera of Australian orchids (<i>Caladenia</i>, <i>Eriochilus</i>, <i>Elythranthera</i>, and <i>Glossodia</i>).
Conclusions: We obtained highly informative DNA markers that will allow investigation of mycorrhizal diversity of Sebacinaceae fungi associated with terrestrial orchids in Australia and worldwide. |
| first_indexed | 2024-12-22T04:05:02Z |
| format | Article |
| id | doaj.art-bdbfc4f99bcc49209debabd2ab0c377f |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-22T04:05:02Z |
| publishDate | 2014-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-bdbfc4f99bcc49209debabd2ab0c377f2022-12-21T18:39:39ZengWileyApplications in Plant Sciences2168-04502014-06-0126140001510.3732/apps.1400015Development of Phylogenetic Markers for <i>Sebacina</i> (Sebacinaceae) Mycorrhizal Fungi Associated with Australian OrchidsMonica P. Ruibal0Rod Peakall1Sylvain Foret2Celeste C. Linde3Evolution, Ecology and Genetics, Research School of Biology, The Australian National University, Canberra, ACT 0200, AustraliaEvolution, Ecology and Genetics, Research School of Biology, The Australian National University, Canberra, ACT 0200, AustraliaEvolution, Ecology and Genetics, Research School of Biology, The Australian National University, Canberra, ACT 0200, AustraliaEvolution, Ecology and Genetics, Research School of Biology, The Australian National University, Canberra, ACT 0200, AustraliaPremise of the study: To investigate fungal species identity and diversity in mycorrhizal fungi of order Sebacinales, we developed phylogenetic markers. These new markers will enable future studies investigating species delineation and phylogenetic relationships of the fungal symbionts and facilitate investigations into evolutionary interactions among <i>Sebacina</i> species and their orchid hosts. Methods and Results: We generated partial genome sequences for a <i>Sebacina</i> symbiont originating from <i>Caladenia huegelii</i> with 454 genome sequencing and from three symbionts from <i>Eriochilus dilatatus</i> and one from <i>E. pulchellus</i> using Illumina sequencing. Six nuclear and two mitochondrial loci showed high variability (10–31% parsimony informative sites) for Sebacinales mycorrhizal fungi across four genera of Australian orchids (<i>Caladenia</i>, <i>Eriochilus</i>, <i>Elythranthera</i>, and <i>Glossodia</i>). Conclusions: We obtained highly informative DNA markers that will allow investigation of mycorrhizal diversity of Sebacinaceae fungi associated with terrestrial orchids in Australia and worldwide.http://www.bioone.org/doi/full/10.3732/apps.1400015mycorrhizal fungiorchidsphylogeneticsSebacina |
| spellingShingle | Monica P. Ruibal Rod Peakall Sylvain Foret Celeste C. Linde Development of Phylogenetic Markers for <i>Sebacina</i> (Sebacinaceae) Mycorrhizal Fungi Associated with Australian Orchids Applications in Plant Sciences mycorrhizal fungi orchids phylogenetics Sebacina |
| title | Development of Phylogenetic Markers for <i>Sebacina</i> (Sebacinaceae) Mycorrhizal Fungi Associated with Australian Orchids |
| title_full | Development of Phylogenetic Markers for <i>Sebacina</i> (Sebacinaceae) Mycorrhizal Fungi Associated with Australian Orchids |
| title_fullStr | Development of Phylogenetic Markers for <i>Sebacina</i> (Sebacinaceae) Mycorrhizal Fungi Associated with Australian Orchids |
| title_full_unstemmed | Development of Phylogenetic Markers for <i>Sebacina</i> (Sebacinaceae) Mycorrhizal Fungi Associated with Australian Orchids |
| title_short | Development of Phylogenetic Markers for <i>Sebacina</i> (Sebacinaceae) Mycorrhizal Fungi Associated with Australian Orchids |
| title_sort | development of phylogenetic markers for i sebacina i sebacinaceae mycorrhizal fungi associated with australian orchids |
| topic | mycorrhizal fungi orchids phylogenetics Sebacina |
| url | http://www.bioone.org/doi/full/10.3732/apps.1400015 |
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