<i>Euodia daniellii</i> Hemsl. Extract and Its Active Component Hesperidin Accelerate Cutaneous Wound Healing via Activation of Wnt/β-Catenin Signaling Pathway
The activation of the Wnt/β-catenin signaling pathway plays a key role in the wound-healing process through tissue regeneration. The extract of <i>Euodia daniellii</i> Hemsl. (<i>E. daniellii</i>), a member of the Rutaceae family, activates the Wnt/β-catenin signaling pathway...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/20/7134 |
| _version_ | 1797470776608161792 |
|---|---|
| author | Minguen Yoon Seol Hwa Seo Seonghwi Choi Gyoonhee Han Kang-Yell Choi |
| author_facet | Minguen Yoon Seol Hwa Seo Seonghwi Choi Gyoonhee Han Kang-Yell Choi |
| author_sort | Minguen Yoon |
| collection | DOAJ |
| description | The activation of the Wnt/β-catenin signaling pathway plays a key role in the wound-healing process through tissue regeneration. The extract of <i>Euodia daniellii</i> Hemsl. (<i>E. daniellii</i>), a member of the Rutaceae family, activates the Wnt/β-catenin signaling pathway. However, the function of <i>E. daniellii</i> in wound healing has not yet been elucidated. We performed a migration assay to determine the wound-healing effect of <i>E. daniellii</i> extract in vitro using human keratinocytes and dermal fibroblast. In addition, a mouse acute wound model was used to investigate the cutaneous wound-healing effect of <i>E. daniellii</i> extract in vivo and confirm the potential mechanism. <i>E. daniellii</i> extract enhanced the migration of human keratinocytes and dermal fibroblasts via the activation of the Wnt/β-catenin pathway. Moreover, the <i>E. daniellii</i> extract increased the levels of keratin 14, PCNA, collagen I, and α-SMA, with nuclei accumulation of β-catenin in vitro. <i>E. daniellii</i> extract also efficiently accelerated re-epithelialization and stimulated wound healing in vivo. Furthermore, we confirmed that hesperidin, one of the components of <i>E. daniellii</i>, efficiently accelerated the migration of human keratinocytes and dermal fibroblasts, as well as wound healing in vivo via the activation of the Wnt/β-catenin pathway. Overall, <i>E. daniellii</i> extract and its active component, hesperidin, have potential to be used as therapeutic agents for wound healing. |
| first_indexed | 2024-03-09T19:41:42Z |
| format | Article |
| id | doaj.art-bdc31b4c5be1452cb74b0ef645cf001e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T19:41:42Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-bdc31b4c5be1452cb74b0ef645cf001e2023-11-24T01:38:06ZengMDPI AGMolecules1420-30492022-10-012720713410.3390/molecules27207134<i>Euodia daniellii</i> Hemsl. Extract and Its Active Component Hesperidin Accelerate Cutaneous Wound Healing via Activation of Wnt/β-Catenin Signaling PathwayMinguen Yoon0Seol Hwa Seo1Seonghwi Choi2Gyoonhee Han3Kang-Yell Choi4Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, KoreaDepartment of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, KoreaDepartment of Interdisciplinary Program of Integreated OMICS for Biomedical Sciences, Yonsei University, Seoul 03722, KoreaDepartment of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, KoreaDepartment of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, KoreaThe activation of the Wnt/β-catenin signaling pathway plays a key role in the wound-healing process through tissue regeneration. The extract of <i>Euodia daniellii</i> Hemsl. (<i>E. daniellii</i>), a member of the Rutaceae family, activates the Wnt/β-catenin signaling pathway. However, the function of <i>E. daniellii</i> in wound healing has not yet been elucidated. We performed a migration assay to determine the wound-healing effect of <i>E. daniellii</i> extract in vitro using human keratinocytes and dermal fibroblast. In addition, a mouse acute wound model was used to investigate the cutaneous wound-healing effect of <i>E. daniellii</i> extract in vivo and confirm the potential mechanism. <i>E. daniellii</i> extract enhanced the migration of human keratinocytes and dermal fibroblasts via the activation of the Wnt/β-catenin pathway. Moreover, the <i>E. daniellii</i> extract increased the levels of keratin 14, PCNA, collagen I, and α-SMA, with nuclei accumulation of β-catenin in vitro. <i>E. daniellii</i> extract also efficiently accelerated re-epithelialization and stimulated wound healing in vivo. Furthermore, we confirmed that hesperidin, one of the components of <i>E. daniellii</i>, efficiently accelerated the migration of human keratinocytes and dermal fibroblasts, as well as wound healing in vivo via the activation of the Wnt/β-catenin pathway. Overall, <i>E. daniellii</i> extract and its active component, hesperidin, have potential to be used as therapeutic agents for wound healing.https://www.mdpi.com/1420-3049/27/20/7134<i>Euodia daniellii</i> Hemsl. extracthesperidinhuman keratinocytehuman dermal fibroblastcutaneous wound healingWnt/β-catenin signaling pathway |
| spellingShingle | Minguen Yoon Seol Hwa Seo Seonghwi Choi Gyoonhee Han Kang-Yell Choi <i>Euodia daniellii</i> Hemsl. Extract and Its Active Component Hesperidin Accelerate Cutaneous Wound Healing via Activation of Wnt/β-Catenin Signaling Pathway Molecules <i>Euodia daniellii</i> Hemsl. extract hesperidin human keratinocyte human dermal fibroblast cutaneous wound healing Wnt/β-catenin signaling pathway |
| title | <i>Euodia daniellii</i> Hemsl. Extract and Its Active Component Hesperidin Accelerate Cutaneous Wound Healing via Activation of Wnt/β-Catenin Signaling Pathway |
| title_full | <i>Euodia daniellii</i> Hemsl. Extract and Its Active Component Hesperidin Accelerate Cutaneous Wound Healing via Activation of Wnt/β-Catenin Signaling Pathway |
| title_fullStr | <i>Euodia daniellii</i> Hemsl. Extract and Its Active Component Hesperidin Accelerate Cutaneous Wound Healing via Activation of Wnt/β-Catenin Signaling Pathway |
| title_full_unstemmed | <i>Euodia daniellii</i> Hemsl. Extract and Its Active Component Hesperidin Accelerate Cutaneous Wound Healing via Activation of Wnt/β-Catenin Signaling Pathway |
| title_short | <i>Euodia daniellii</i> Hemsl. Extract and Its Active Component Hesperidin Accelerate Cutaneous Wound Healing via Activation of Wnt/β-Catenin Signaling Pathway |
| title_sort | i euodia daniellii i hemsl extract and its active component hesperidin accelerate cutaneous wound healing via activation of wnt β catenin signaling pathway |
| topic | <i>Euodia daniellii</i> Hemsl. extract hesperidin human keratinocyte human dermal fibroblast cutaneous wound healing Wnt/β-catenin signaling pathway |
| url | https://www.mdpi.com/1420-3049/27/20/7134 |
| work_keys_str_mv | AT minguenyoon ieuodiadanielliiihemslextractanditsactivecomponenthesperidinacceleratecutaneouswoundhealingviaactivationofwntbcateninsignalingpathway AT seolhwaseo ieuodiadanielliiihemslextractanditsactivecomponenthesperidinacceleratecutaneouswoundhealingviaactivationofwntbcateninsignalingpathway AT seonghwichoi ieuodiadanielliiihemslextractanditsactivecomponenthesperidinacceleratecutaneouswoundhealingviaactivationofwntbcateninsignalingpathway AT gyoonheehan ieuodiadanielliiihemslextractanditsactivecomponenthesperidinacceleratecutaneouswoundhealingviaactivationofwntbcateninsignalingpathway AT kangyellchoi ieuodiadanielliiihemslextractanditsactivecomponenthesperidinacceleratecutaneouswoundhealingviaactivationofwntbcateninsignalingpathway |