Identification and expression analysis of bovine ANGPTL gene family
Encoded by seven genes, angiopoietin-like (ANGPTL) family members structurally similar to the angiogenic regulating factor angiopoietin are known to possess biological activities in angiogenesis and metabolism. Here we reports for the first time the identification and expression analysis of all the...
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| Format: | Article |
| Language: | English |
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Oxford University Press
2010-08-01
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| Series: | Current Zoology |
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| Online Access: | http://www.currentzoology.org/paperdetail.asp?id=11556 |
| _version_ | 1818512554130931712 |
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| author | Zhengbing GUAN,Guolin CAI, Junyong SUN, Jian LU |
| author_facet | Zhengbing GUAN,Guolin CAI, Junyong SUN, Jian LU |
| author_sort | Zhengbing GUAN,Guolin CAI, Junyong SUN, Jian LU |
| collection | DOAJ |
| description | Encoded by seven genes, angiopoietin-like (ANGPTL) family members structurally similar to the angiogenic regulating factor angiopoietin are known to possess biological activities in angiogenesis and metabolism. Here we reports for the first time the identification and expression analysis of all the seven members of bovine ANGPTL gene family, which were designated bANGPTL1 to bANGPTL7 in order. The seven bANGPTL genes consist of 4–9 exons, span 3800–43000 bp and are located on different chromosomes. The deduced amino acid sequences of the members all possess an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, both characteristics of angiopoietins. Phylogenetic analysis showed that the 32 identified ANGPTL homologs from 9 species could be classified into two major groups. Real-time quantitative PCR (Q-PCR) analysis revealed that the bANGPTL family members have different expression patterns. This study will be helpful for investigation on the biological role of the bANGPTL family in this economically important species. Furthermore, it provides an insight into the molecular evolution of the emerging ANGPTL family [Current Zoology 56 (4): 445–453, 2010]. |
| first_indexed | 2024-12-10T23:48:28Z |
| format | Article |
| id | doaj.art-bdc385b1d3014c728804e27942bd578a |
| institution | Directory Open Access Journal |
| issn | 1674-5507 |
| language | English |
| last_indexed | 2024-12-10T23:48:28Z |
| publishDate | 2010-08-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Current Zoology |
| spelling | doaj.art-bdc385b1d3014c728804e27942bd578a2022-12-22T01:28:51ZengOxford University PressCurrent Zoology1674-55072010-08-01564445453Identification and expression analysis of bovine ANGPTL gene familyZhengbing GUAN,Guolin CAI, Junyong SUN, Jian LUEncoded by seven genes, angiopoietin-like (ANGPTL) family members structurally similar to the angiogenic regulating factor angiopoietin are known to possess biological activities in angiogenesis and metabolism. Here we reports for the first time the identification and expression analysis of all the seven members of bovine ANGPTL gene family, which were designated bANGPTL1 to bANGPTL7 in order. The seven bANGPTL genes consist of 4–9 exons, span 3800–43000 bp and are located on different chromosomes. The deduced amino acid sequences of the members all possess an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, both characteristics of angiopoietins. Phylogenetic analysis showed that the 32 identified ANGPTL homologs from 9 species could be classified into two major groups. Real-time quantitative PCR (Q-PCR) analysis revealed that the bANGPTL family members have different expression patterns. This study will be helpful for investigation on the biological role of the bANGPTL family in this economically important species. Furthermore, it provides an insight into the molecular evolution of the emerging ANGPTL family [Current Zoology 56 (4): 445–453, 2010].http://www.currentzoology.org/paperdetail.asp?id=11556CattleAngiopoietin-likePhylogenetic analysisQ-PCR |
| spellingShingle | Zhengbing GUAN,Guolin CAI, Junyong SUN, Jian LU Identification and expression analysis of bovine ANGPTL gene family Current Zoology Cattle Angiopoietin-like Phylogenetic analysis Q-PCR |
| title | Identification and expression analysis of bovine ANGPTL gene family |
| title_full | Identification and expression analysis of bovine ANGPTL gene family |
| title_fullStr | Identification and expression analysis of bovine ANGPTL gene family |
| title_full_unstemmed | Identification and expression analysis of bovine ANGPTL gene family |
| title_short | Identification and expression analysis of bovine ANGPTL gene family |
| title_sort | identification and expression analysis of bovine angptl gene family |
| topic | Cattle Angiopoietin-like Phylogenetic analysis Q-PCR |
| url | http://www.currentzoology.org/paperdetail.asp?id=11556 |
| work_keys_str_mv | AT zhengbingguanguolincaijunyongsunjianlu identificationandexpressionanalysisofbovineangptlgenefamily |