Transcriptional Cascade in the Regulation of Flowering in the Bamboo Orchid <i>Arundina graminifolia</i>
Flowering in orchids is the most important horticultural trait regulated by multiple mechanisms. <i>Arundina graminifolia</i> flowers throughout the year unlike other orchids with a narrow flowering span. However, little is known of the genetic regulation of this peculiar flowering patte...
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MDPI AG
2021-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/6/771 |
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| author | Sagheer Ahmad Chuqiao Lu Jieqiu Wu Yonglu Wei Jie Gao Jianpeng Jin Chuanyuan Zheng Genfa Zhu Fengxi Yang |
| author_facet | Sagheer Ahmad Chuqiao Lu Jieqiu Wu Yonglu Wei Jie Gao Jianpeng Jin Chuanyuan Zheng Genfa Zhu Fengxi Yang |
| author_sort | Sagheer Ahmad |
| collection | DOAJ |
| description | Flowering in orchids is the most important horticultural trait regulated by multiple mechanisms. <i>Arundina graminifolia</i> flowers throughout the year unlike other orchids with a narrow flowering span. However, little is known of the genetic regulation of this peculiar flowering pattern. This study identifies a number of transcription factor (TF) families in five stages of flower development and four tissue types through RNA-seq transcriptome. About 700 DEGs were annotated to the transcription factor category and classified into 35 TF families, which were involved in multiple signaling pathways. The most abundant TF family was bHLH, followed by MYB and WRKY. Some important members of the bHLH, WRKY, MYB, TCP, and MADS-box families were found to regulate the flowering genes at transcriptional levels. Particularly, the TFs WRKY34 and ERF12 possibly respond to vernalization and photoperiod signaling, MYB108, RR9, VP1, and bHLH49 regulate hormonal balance, and CCA1 may control the circadian pathway. MADS-box TFs including MADS6, 14, 16, AGL5, and SEP may be important regulators of flowering in <i>A. graminifolia</i>. Therefore, this study provides a theoretical basis for understanding the molecular mechanism of flowering in <i>A. graminifolia</i>. |
| first_indexed | 2024-03-10T11:11:35Z |
| format | Article |
| id | doaj.art-bdc4189cb5744c4e94b86d63fe6edb98 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T11:11:35Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-bdc4189cb5744c4e94b86d63fe6edb982023-11-21T20:44:44ZengMDPI AGBiomolecules2218-273X2021-05-0111677110.3390/biom11060771Transcriptional Cascade in the Regulation of Flowering in the Bamboo Orchid <i>Arundina graminifolia</i>Sagheer Ahmad0Chuqiao Lu1Jieqiu Wu2Yonglu Wei3Jie Gao4Jianpeng Jin5Chuanyuan Zheng6Genfa Zhu7Fengxi Yang8Guangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaFlowering in orchids is the most important horticultural trait regulated by multiple mechanisms. <i>Arundina graminifolia</i> flowers throughout the year unlike other orchids with a narrow flowering span. However, little is known of the genetic regulation of this peculiar flowering pattern. This study identifies a number of transcription factor (TF) families in five stages of flower development and four tissue types through RNA-seq transcriptome. About 700 DEGs were annotated to the transcription factor category and classified into 35 TF families, which were involved in multiple signaling pathways. The most abundant TF family was bHLH, followed by MYB and WRKY. Some important members of the bHLH, WRKY, MYB, TCP, and MADS-box families were found to regulate the flowering genes at transcriptional levels. Particularly, the TFs WRKY34 and ERF12 possibly respond to vernalization and photoperiod signaling, MYB108, RR9, VP1, and bHLH49 regulate hormonal balance, and CCA1 may control the circadian pathway. MADS-box TFs including MADS6, 14, 16, AGL5, and SEP may be important regulators of flowering in <i>A. graminifolia</i>. Therefore, this study provides a theoretical basis for understanding the molecular mechanism of flowering in <i>A. graminifolia</i>.https://www.mdpi.com/2218-273X/11/6/771<i>Arundina graminifolia</i>floweringtranscriptometranscription factor |
| spellingShingle | Sagheer Ahmad Chuqiao Lu Jieqiu Wu Yonglu Wei Jie Gao Jianpeng Jin Chuanyuan Zheng Genfa Zhu Fengxi Yang Transcriptional Cascade in the Regulation of Flowering in the Bamboo Orchid <i>Arundina graminifolia</i> Biomolecules <i>Arundina graminifolia</i> flowering transcriptome transcription factor |
| title | Transcriptional Cascade in the Regulation of Flowering in the Bamboo Orchid <i>Arundina graminifolia</i> |
| title_full | Transcriptional Cascade in the Regulation of Flowering in the Bamboo Orchid <i>Arundina graminifolia</i> |
| title_fullStr | Transcriptional Cascade in the Regulation of Flowering in the Bamboo Orchid <i>Arundina graminifolia</i> |
| title_full_unstemmed | Transcriptional Cascade in the Regulation of Flowering in the Bamboo Orchid <i>Arundina graminifolia</i> |
| title_short | Transcriptional Cascade in the Regulation of Flowering in the Bamboo Orchid <i>Arundina graminifolia</i> |
| title_sort | transcriptional cascade in the regulation of flowering in the bamboo orchid i arundina graminifolia i |
| topic | <i>Arundina graminifolia</i> flowering transcriptome transcription factor |
| url | https://www.mdpi.com/2218-273X/11/6/771 |
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