Physiological Response of In Vitro Cultured MAGNOLIASP. to Nutrient Medium Composition
The objective of this study was to assess the regeneration response of in vitro cultured Magnolia × soulangeana ‘Alexandrina’ and Magnolia liliiflora ‘Nigra’ to nutrient medium composition. In the primary culture (initiated from dormant axillary buds) combinations of Murashige and Skoog (MS) basal s...
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| Format: | Article |
| Language: | English |
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Sciendo
2014-09-01
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| Series: | Journal of Horticultural Research |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/johr-2014-0006 |
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| author | S. Sokolov Rossen Y. Atanassova Bistra T. Iakimova Elena |
| author_facet | S. Sokolov Rossen Y. Atanassova Bistra T. Iakimova Elena |
| author_sort | S. Sokolov Rossen |
| collection | DOAJ |
| description | The objective of this study was to assess the regeneration response of in vitro cultured Magnolia × soulangeana ‘Alexandrina’ and Magnolia liliiflora ‘Nigra’ to nutrient medium composition. In the primary culture (initiated from dormant axillary buds) combinations of Murashige and Skoog (MS) basal salts with 6-benzylaminopurine and α-naphthaleneacetic acid were tested. The primary explants of cv. ‘Alexandrina’ expressed higher regeneration rate than cv. ‘Nigra’. For both species, the regen eration was most strongly potentiated at addition of 0.25 mg dm−3 of the cytokinin alone. The auxin exerted undesir–able effects. Several basal salts media were applied in proliferation stage and their physiological effects were evaluated in reference to traditionally used MS. At culturing on Chée & Pool C2d Vitis Medium (VM) that is for the first time introduced to magnolia and on MS, M. liliiflora formed more but less elongated shoots than M. soulangeana. However, on VM, substantial increase (25-30%) of the number of axillary shoots and leaves, shoot length and fresh and dry weights over MS was established for both species. This suggested VM as promising composition of nutrients in multiplication stage. Microshoots obtained on MS, VM, Rugini Olive Medium and DKW Juglans Medium were successfully rooted in vitro and subsequently established ex vitro. The findings expand the information on magnolia response to culture conditions and contribute to elaboration of innovative elements of protocols for establishing tissue cultures with high regeneration capacity. |
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| id | doaj.art-bdc9f19084b84065aae9b1f033beeb56 |
| institution | Directory Open Access Journal |
| issn | 2300-5009 |
| language | English |
| last_indexed | 2024-12-13T16:30:22Z |
| publishDate | 2014-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Horticultural Research |
| spelling | doaj.art-bdc9f19084b84065aae9b1f033beeb562022-12-21T23:38:32ZengSciendoJournal of Horticultural Research2300-50092014-09-01221496110.2478/johr-2014-0006johr-2014-0006Physiological Response of In Vitro Cultured MAGNOLIASP. to Nutrient Medium CompositionS. Sokolov Rossen0Y. Atanassova Bistra1T. Iakimova Elena2Institute of Ornamental Plants, 1222 Negovan, Sofia, BulgariaInstitute of Ornamental Plants, 1222 Negovan, Sofia, BulgariaInstitute of Ornamental Plants, 1222 Negovan, Sofia, BulgariaThe objective of this study was to assess the regeneration response of in vitro cultured Magnolia × soulangeana ‘Alexandrina’ and Magnolia liliiflora ‘Nigra’ to nutrient medium composition. In the primary culture (initiated from dormant axillary buds) combinations of Murashige and Skoog (MS) basal salts with 6-benzylaminopurine and α-naphthaleneacetic acid were tested. The primary explants of cv. ‘Alexandrina’ expressed higher regeneration rate than cv. ‘Nigra’. For both species, the regen eration was most strongly potentiated at addition of 0.25 mg dm−3 of the cytokinin alone. The auxin exerted undesir–able effects. Several basal salts media were applied in proliferation stage and their physiological effects were evaluated in reference to traditionally used MS. At culturing on Chée & Pool C2d Vitis Medium (VM) that is for the first time introduced to magnolia and on MS, M. liliiflora formed more but less elongated shoots than M. soulangeana. However, on VM, substantial increase (25-30%) of the number of axillary shoots and leaves, shoot length and fresh and dry weights over MS was established for both species. This suggested VM as promising composition of nutrients in multiplication stage. Microshoots obtained on MS, VM, Rugini Olive Medium and DKW Juglans Medium were successfully rooted in vitro and subsequently established ex vitro. The findings expand the information on magnolia response to culture conditions and contribute to elaboration of innovative elements of protocols for establishing tissue cultures with high regeneration capacity.https://doi.org/10.2478/johr-2014-0006magnolia sp.basal mediumplant growth regulatorsprimary culturerootingshoot proliferation |
| spellingShingle | S. Sokolov Rossen Y. Atanassova Bistra T. Iakimova Elena Physiological Response of In Vitro Cultured MAGNOLIASP. to Nutrient Medium Composition Journal of Horticultural Research magnolia sp. basal medium plant growth regulators primary culture rooting shoot proliferation |
| title | Physiological Response of In Vitro Cultured MAGNOLIASP. to Nutrient Medium Composition |
| title_full | Physiological Response of In Vitro Cultured MAGNOLIASP. to Nutrient Medium Composition |
| title_fullStr | Physiological Response of In Vitro Cultured MAGNOLIASP. to Nutrient Medium Composition |
| title_full_unstemmed | Physiological Response of In Vitro Cultured MAGNOLIASP. to Nutrient Medium Composition |
| title_short | Physiological Response of In Vitro Cultured MAGNOLIASP. to Nutrient Medium Composition |
| title_sort | physiological response of in vitro cultured magnoliasp to nutrient medium composition |
| topic | magnolia sp. basal medium plant growth regulators primary culture rooting shoot proliferation |
| url | https://doi.org/10.2478/johr-2014-0006 |
| work_keys_str_mv | AT ssokolovrossen physiologicalresponseofinvitroculturedmagnoliasptonutrientmediumcomposition AT yatanassovabistra physiologicalresponseofinvitroculturedmagnoliasptonutrientmediumcomposition AT tiakimovaelena physiologicalresponseofinvitroculturedmagnoliasptonutrientmediumcomposition |