Degree of Fibrosis in Human Atrial Tissue Is Not the Hallmark Driving AF
Background: The current paradigm is that fibrosis promotes electrophysiological disorders and drives atrial fibrillation (AF). In this current study, we investigated the relation between the degree of fibrosis in human atrial tissue samples of controls and patients in various stages of AF and the de...
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MDPI AG
2022-01-01
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| author | Kennedy S. Ramos Lisa Pool Mathijs S. van Schie Leonoor F. J. M. Wijdeveld Willemijn F. B. van der Does Luciënne Baks H. M. Danish Sultan Stan W. van Wijk Ad J. J. C. Bogers Sander Verheule Natasja M. S. de Groot Bianca J. J. M. Brundel |
| author_facet | Kennedy S. Ramos Lisa Pool Mathijs S. van Schie Leonoor F. J. M. Wijdeveld Willemijn F. B. van der Does Luciënne Baks H. M. Danish Sultan Stan W. van Wijk Ad J. J. C. Bogers Sander Verheule Natasja M. S. de Groot Bianca J. J. M. Brundel |
| author_sort | Kennedy S. Ramos |
| collection | DOAJ |
| description | Background: The current paradigm is that fibrosis promotes electrophysiological disorders and drives atrial fibrillation (AF). In this current study, we investigated the relation between the degree of fibrosis in human atrial tissue samples of controls and patients in various stages of AF and the degree of electrophysiological abnormalities. Methods: The degree of fibrosis was measured in the atrial tissue and serum of patients in various stages of AF and the controls. Hereto, picrosirius and H&E staining were performed to quantify degree of total, endo-perimysial fibrosis, and cardiomyocyte diameter. Western blot quantified fibrosis markers: neural cell adhesion molecule, tissue inhibitor of metalloproteinase, lysyl oxidase, and α-smooth muscle actin. In serum, the ratio carboxyl-terminal telopeptide of collagen/matrix-metalloproteinase1 was determined. High-resolution epicardial mapping evaluated low-voltage areas and conduction abnormalities. Results: No significant differences were observed in the degree of fibrosis between the groups. Finally, no significant correlation—absolute nor spatial—was observed between all electrophysiological parameters and histological fibrosis markers. Conclusions: No differences in the degree of fibrosis were observed in patients from various stages of AF compared to the controls. Moreover, electrophysiological abnormalities did not correlate with any of the fibrosis markers. The findings indicate that fibrosis is not the hallmark of structural remodeling in AF. |
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| spelling | doaj.art-bdcc1e1d669248ffb364e8383469ecf02023-11-23T16:11:40ZengMDPI AGCells2073-44092022-01-0111342710.3390/cells11030427Degree of Fibrosis in Human Atrial Tissue Is Not the Hallmark Driving AFKennedy S. Ramos0Lisa Pool1Mathijs S. van Schie2Leonoor F. J. M. Wijdeveld3Willemijn F. B. van der Does4Luciënne Baks5H. M. Danish Sultan6Stan W. van Wijk7Ad J. J. C. Bogers8Sander Verheule9Natasja M. S. de Groot10Bianca J. J. M. Brundel11Department of Physiology, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centers, 1081 HV Amsterdam, The NetherlandsDepartment of Physiology, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centers, 1081 HV Amsterdam, The NetherlandsDepartment Cardiology, Erasmus Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Physiology, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centers, 1081 HV Amsterdam, The NetherlandsDepartment Cardiology, Erasmus Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Physiology, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centers, 1081 HV Amsterdam, The NetherlandsDepartment of Physiology, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centers, 1081 HV Amsterdam, The NetherlandsDepartment of Physiology, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centers, 1081 HV Amsterdam, The NetherlandsDepartment of Cardiothoracic Surgery, Erasmus Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Physiology, University Maastricht, 6211 LK Maastricht, The NetherlandsDepartment Cardiology, Erasmus Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Physiology, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centers, 1081 HV Amsterdam, The NetherlandsBackground: The current paradigm is that fibrosis promotes electrophysiological disorders and drives atrial fibrillation (AF). In this current study, we investigated the relation between the degree of fibrosis in human atrial tissue samples of controls and patients in various stages of AF and the degree of electrophysiological abnormalities. Methods: The degree of fibrosis was measured in the atrial tissue and serum of patients in various stages of AF and the controls. Hereto, picrosirius and H&E staining were performed to quantify degree of total, endo-perimysial fibrosis, and cardiomyocyte diameter. Western blot quantified fibrosis markers: neural cell adhesion molecule, tissue inhibitor of metalloproteinase, lysyl oxidase, and α-smooth muscle actin. In serum, the ratio carboxyl-terminal telopeptide of collagen/matrix-metalloproteinase1 was determined. High-resolution epicardial mapping evaluated low-voltage areas and conduction abnormalities. Results: No significant differences were observed in the degree of fibrosis between the groups. Finally, no significant correlation—absolute nor spatial—was observed between all electrophysiological parameters and histological fibrosis markers. Conclusions: No differences in the degree of fibrosis were observed in patients from various stages of AF compared to the controls. Moreover, electrophysiological abnormalities did not correlate with any of the fibrosis markers. The findings indicate that fibrosis is not the hallmark of structural remodeling in AF.https://www.mdpi.com/2073-4409/11/3/427atrial fibrillationfibrosis(bio)markerscardiac mappingstructural remodelingelectrical remodeling |
| spellingShingle | Kennedy S. Ramos Lisa Pool Mathijs S. van Schie Leonoor F. J. M. Wijdeveld Willemijn F. B. van der Does Luciënne Baks H. M. Danish Sultan Stan W. van Wijk Ad J. J. C. Bogers Sander Verheule Natasja M. S. de Groot Bianca J. J. M. Brundel Degree of Fibrosis in Human Atrial Tissue Is Not the Hallmark Driving AF Cells atrial fibrillation fibrosis (bio)markers cardiac mapping structural remodeling electrical remodeling |
| title | Degree of Fibrosis in Human Atrial Tissue Is Not the Hallmark Driving AF |
| title_full | Degree of Fibrosis in Human Atrial Tissue Is Not the Hallmark Driving AF |
| title_fullStr | Degree of Fibrosis in Human Atrial Tissue Is Not the Hallmark Driving AF |
| title_full_unstemmed | Degree of Fibrosis in Human Atrial Tissue Is Not the Hallmark Driving AF |
| title_short | Degree of Fibrosis in Human Atrial Tissue Is Not the Hallmark Driving AF |
| title_sort | degree of fibrosis in human atrial tissue is not the hallmark driving af |
| topic | atrial fibrillation fibrosis (bio)markers cardiac mapping structural remodeling electrical remodeling |
| url | https://www.mdpi.com/2073-4409/11/3/427 |
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