Cervical anticancer activities of Annona squamosa Linn. leaf isolate
Background and Aim: Cancer is one of the leading causes of death, the need for new anticancer herbal drugs is becoming more urgent considering the side effects of synthetic drugs. This study aimed to determine the anticancer activity of isolates derived from the methanol extract of Annona squamosa L...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Veterinary World
2022-01-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.15/January-2022/16.pdf |
| _version_ | 1818973942745923584 |
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| author | Made Dira Swantara Wiwik Susanah Rita Made Asmarani Dira Kadek Karang Agustina |
| author_facet | Made Dira Swantara Wiwik Susanah Rita Made Asmarani Dira Kadek Karang Agustina |
| author_sort | Made Dira Swantara |
| collection | DOAJ |
| description | Background and Aim: Cancer is one of the leading causes of death, the need for new anticancer herbal drugs is becoming more urgent considering the side effects of synthetic drugs. This study aimed to determine the anticancer activity of isolates derived from the methanol extract of Annona squamosa Linn. leaves and to identify the compounds that have an active effect against HeLa cells.
Materials and Methods: The leaf metabolites of A. squamosa L. were extracted using methanol at room temperature (28°C) and were partitioned into n-hexane, chloroform, and n-butanol. The toxicity test of these extracts was conducted using a brine shrimp lethality assay. Furthermore, the most toxic extracts were separated and purified using silica gel column chromatography to yield four isolate fractions: FA, FB, FC, and FD. The most toxic isolates were tested for anticancer against HeLa cells, and their compounds were identified using liquid chromatography-mass spectrometry.
Results: The results showed that the most toxic isolate with an LC50 value of 100.00 ppm had a potency similar to that of an anticancer agent with an IC50 value of 70.9021 ppm. Furthermore, the five compounds identified in this isolate include (6S, 7aR)-6-hydroxy-4,4,7a-trimethyl-6,7-dihydro-5H-1-benzofuran-2-one or loliolide, cocamidopropyl betaine, N-[3- (dimethylamino)propyl]dodecanamide or lauramidopropyl dimethylamine, linolenic acid, and 1-dodecyl-2-azepanone or laurocapram.
Conclusion: It can be concluded that the leaf isolates of A. squamosa Linn. had shown anticancer activities against cervical cancer. |
| first_indexed | 2024-12-20T15:32:11Z |
| format | Article |
| id | doaj.art-bdcfbcff43ee4880bf40cdbf04039d9a |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-20T15:32:11Z |
| publishDate | 2022-01-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-bdcfbcff43ee4880bf40cdbf04039d9a2022-12-21T19:35:34ZengVeterinary WorldVeterinary World0972-89882231-09162022-01-0115112413110.14202/vetworld.2022.124-131Cervical anticancer activities of Annona squamosa Linn. leaf isolateMade Dira Swantara0https://orcid.org/0000-0002-2798-3321Wiwik Susanah Rita1https://orcid.org/0000-0001-8040-1645Made Asmarani Dira2https://orcid.org/0000-0002-1583-1099Kadek Karang Agustina3https://orcid.org/0000-0002-7128-0914Department of Applied Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University, Denpasar, Bali 80225 Indonesia.Department of Applied Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University, Denpasar, Bali 80225 Indonesia.Clinical and Community Pharmacy Study Program, Faculty of Health, Bali Institute of Technology and Health, Denpasar, Bali 80225, Indonesia.Department of Public Health, The Faculty of Veterinary Medicine, Udayana University, Denpasar, Bali 80225, Indonesia.Background and Aim: Cancer is one of the leading causes of death, the need for new anticancer herbal drugs is becoming more urgent considering the side effects of synthetic drugs. This study aimed to determine the anticancer activity of isolates derived from the methanol extract of Annona squamosa Linn. leaves and to identify the compounds that have an active effect against HeLa cells. Materials and Methods: The leaf metabolites of A. squamosa L. were extracted using methanol at room temperature (28°C) and were partitioned into n-hexane, chloroform, and n-butanol. The toxicity test of these extracts was conducted using a brine shrimp lethality assay. Furthermore, the most toxic extracts were separated and purified using silica gel column chromatography to yield four isolate fractions: FA, FB, FC, and FD. The most toxic isolates were tested for anticancer against HeLa cells, and their compounds were identified using liquid chromatography-mass spectrometry. Results: The results showed that the most toxic isolate with an LC50 value of 100.00 ppm had a potency similar to that of an anticancer agent with an IC50 value of 70.9021 ppm. Furthermore, the five compounds identified in this isolate include (6S, 7aR)-6-hydroxy-4,4,7a-trimethyl-6,7-dihydro-5H-1-benzofuran-2-one or loliolide, cocamidopropyl betaine, N-[3- (dimethylamino)propyl]dodecanamide or lauramidopropyl dimethylamine, linolenic acid, and 1-dodecyl-2-azepanone or laurocapram. Conclusion: It can be concluded that the leaf isolates of A. squamosa Linn. had shown anticancer activities against cervical cancer.http://www.veterinaryworld.org/Vol.15/January-2022/16.pdfannona squamosa linncervical anticancer activitieshela cells |
| spellingShingle | Made Dira Swantara Wiwik Susanah Rita Made Asmarani Dira Kadek Karang Agustina Cervical anticancer activities of Annona squamosa Linn. leaf isolate Veterinary World annona squamosa linn cervical anticancer activities hela cells |
| title | Cervical anticancer activities of Annona squamosa Linn. leaf isolate |
| title_full | Cervical anticancer activities of Annona squamosa Linn. leaf isolate |
| title_fullStr | Cervical anticancer activities of Annona squamosa Linn. leaf isolate |
| title_full_unstemmed | Cervical anticancer activities of Annona squamosa Linn. leaf isolate |
| title_short | Cervical anticancer activities of Annona squamosa Linn. leaf isolate |
| title_sort | cervical anticancer activities of annona squamosa linn leaf isolate |
| topic | annona squamosa linn cervical anticancer activities hela cells |
| url | http://www.veterinaryworld.org/Vol.15/January-2022/16.pdf |
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