A role for barley calcium-dependent protein kinase CPK2a in the response to drought
Increasing the drought tolerance of crops is one of the most challenging goals in plant breeding. To improve crop productivity during periods of water deficit, it is essential to understand the complex regulatory pathways that adapt plant metabolism to environmental conditions. Among various plant h...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-10-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01550/full |
| _version_ | 1828296114975014912 |
|---|---|
| author | Agata Cieśla Filip Mituła Lucyna Misztal Olga Fedorowicz-Strońska Sabina Janicka Małgorzata Tajdel-Zielińska Małgorzata Marczak Maciej Janicki Agnieszka Ludwików Jan Sadowski |
| author_facet | Agata Cieśla Filip Mituła Lucyna Misztal Olga Fedorowicz-Strońska Sabina Janicka Małgorzata Tajdel-Zielińska Małgorzata Marczak Maciej Janicki Agnieszka Ludwików Jan Sadowski |
| author_sort | Agata Cieśla |
| collection | DOAJ |
| description | Increasing the drought tolerance of crops is one of the most challenging goals in plant breeding. To improve crop productivity during periods of water deficit, it is essential to understand the complex regulatory pathways that adapt plant metabolism to environmental conditions. Among various plant hormones and second messengers, calcium ions are known to be involved in drought stress perception and signaling. Plants have developed specific calcium-dependent protein kinases that convert calcium signals into phosphorylation events. In this study we attempted to elucidate the role of a calcium-dependent protein kinase in the drought stress response of barley (Hordeum vulgare L.), one of the most economically important crops worldwide. The ongoing barley genome project has provided useful information about genes potentially involved in the drought stress response, but information on the role of calcium-dependent kinases is still limited. We found that the gene encoding the calcium-dependent protein kinase HvCPK2a was significantly upregulated in response to drought. To better understand the role of HvCPK2a in drought stress signaling, we generated transgenic Arabidopsis plants that overexpressed the corresponding coding sequence. Overexpressing lines displayed drought sensitivity, reduced nitrogen balance index, an increase in total chlorophyll content and decreased relative water content. In addition, in vitro kinase assay experiments combined with mass spectrometry allowed HvCPK2a autophosphorylation sites to be identified. Our results suggest that HvCPK2a is a dual-specificity calcium-dependent protein kinase that functions as a negative regulator of the drought stress response in barley. |
| first_indexed | 2024-04-13T12:07:30Z |
| format | Article |
| id | doaj.art-bdd20f7ca63e4c33a6b7db7ba6a23782 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T12:07:30Z |
| publishDate | 2016-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-bdd20f7ca63e4c33a6b7db7ba6a237822022-12-22T02:47:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-10-01710.3389/fpls.2016.01550222815A role for barley calcium-dependent protein kinase CPK2a in the response to droughtAgata Cieśla0Filip Mituła1Lucyna Misztal2Olga Fedorowicz-Strońska3Sabina Janicka4Małgorzata Tajdel-Zielińska5Małgorzata Marczak6Maciej Janicki7Agnieszka Ludwików8Jan Sadowski9Adam Mickiewicz University in PoznańAdam Mickiewicz University in PoznańAdam Mickiewicz University in PoznańPolish Academy of ScienceAdam Mickiewicz University in PoznańAdam Mickiewicz University in PoznańAdam Mickiewicz University in PoznańAdam Mickiewicz University in PoznańAdam Mickiewicz University in PoznańAdam Mickiewicz University in PoznańIncreasing the drought tolerance of crops is one of the most challenging goals in plant breeding. To improve crop productivity during periods of water deficit, it is essential to understand the complex regulatory pathways that adapt plant metabolism to environmental conditions. Among various plant hormones and second messengers, calcium ions are known to be involved in drought stress perception and signaling. Plants have developed specific calcium-dependent protein kinases that convert calcium signals into phosphorylation events. In this study we attempted to elucidate the role of a calcium-dependent protein kinase in the drought stress response of barley (Hordeum vulgare L.), one of the most economically important crops worldwide. The ongoing barley genome project has provided useful information about genes potentially involved in the drought stress response, but information on the role of calcium-dependent kinases is still limited. We found that the gene encoding the calcium-dependent protein kinase HvCPK2a was significantly upregulated in response to drought. To better understand the role of HvCPK2a in drought stress signaling, we generated transgenic Arabidopsis plants that overexpressed the corresponding coding sequence. Overexpressing lines displayed drought sensitivity, reduced nitrogen balance index, an increase in total chlorophyll content and decreased relative water content. In addition, in vitro kinase assay experiments combined with mass spectrometry allowed HvCPK2a autophosphorylation sites to be identified. Our results suggest that HvCPK2a is a dual-specificity calcium-dependent protein kinase that functions as a negative regulator of the drought stress response in barley.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01550/fullMass SpectrometryDrought stressProtein functioncalcium-dependent protein kinaseHordeum vulgare L. |
| spellingShingle | Agata Cieśla Filip Mituła Lucyna Misztal Olga Fedorowicz-Strońska Sabina Janicka Małgorzata Tajdel-Zielińska Małgorzata Marczak Maciej Janicki Agnieszka Ludwików Jan Sadowski A role for barley calcium-dependent protein kinase CPK2a in the response to drought Frontiers in Plant Science Mass Spectrometry Drought stress Protein function calcium-dependent protein kinase Hordeum vulgare L. |
| title | A role for barley calcium-dependent protein kinase CPK2a in the response to drought |
| title_full | A role for barley calcium-dependent protein kinase CPK2a in the response to drought |
| title_fullStr | A role for barley calcium-dependent protein kinase CPK2a in the response to drought |
| title_full_unstemmed | A role for barley calcium-dependent protein kinase CPK2a in the response to drought |
| title_short | A role for barley calcium-dependent protein kinase CPK2a in the response to drought |
| title_sort | role for barley calcium dependent protein kinase cpk2a in the response to drought |
| topic | Mass Spectrometry Drought stress Protein function calcium-dependent protein kinase Hordeum vulgare L. |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01550/full |
| work_keys_str_mv | AT agataciesla aroleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT filipmituła aroleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT lucynamisztal aroleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT olgafedorowiczstronska aroleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT sabinajanicka aroleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT małgorzatatajdelzielinska aroleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT małgorzatamarczak aroleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT maciejjanicki aroleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT agnieszkaludwikow aroleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT jansadowski aroleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT agataciesla roleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT filipmituła roleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT lucynamisztal roleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT olgafedorowiczstronska roleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT sabinajanicka roleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT małgorzatatajdelzielinska roleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT małgorzatamarczak roleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT maciejjanicki roleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT agnieszkaludwikow roleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought AT jansadowski roleforbarleycalciumdependentproteinkinasecpk2aintheresponsetodrought |