Farming Practices Influence Antibiotic Resistance and Biogenic Amine Capacity of Staphylococci from Bulk Tank Ewe’s Milk
Staphylococci are one of the main microorganisms responsible for intramammary infections in sheep, causing important economic losses for farmers and eventually health problems in humans, especially by the consumption of dairy products made with raw milk containing toxic compounds, such as biogenic a...
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MDPI AG
2020-09-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/9/1622 |
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| author | Justa María Poveda Lorena Jiménez José Manuel Perea Ramón Arias María Llanos Palop |
| author_facet | Justa María Poveda Lorena Jiménez José Manuel Perea Ramón Arias María Llanos Palop |
| author_sort | Justa María Poveda |
| collection | DOAJ |
| description | Staphylococci are one of the main microorganisms responsible for intramammary infections in sheep, causing important economic losses for farmers and eventually health problems in humans, especially by the consumption of dairy products made with raw milk containing toxic compounds, such as biogenic amines or antibiotic resistant bacteria. This study aimed to check the presence and safety of staphylococci in bulk tank ewe’s milk from different farms, and to determine the relationship between the presence of these staphylococci and farming practices, by applying nonlinear canonical correlation models (OVERALS). Two-hundred and fifty-nine staphylococci from milk samples from eighteen farms were genotyped and representative isolates of the major clusters were identified as belonging to <i>Staphylococcus (S.) aureus</i>, <i>S. epidermidis</i>, <i>S. arlettae</i>, <i>S. lentus</i>, <i>S. simulans</i>, and <i>S. chromogenes</i> species. Identified isolates were assayed in terms of their safety, by evaluating resistance to antimicrobial drugs and the aminobiogenic capacity, using both phenotypic and genetic assays. Antibiotic resistance phenotypic assay revealed that 82.9% were resistant to some antibiotics, although in the genotypic assay only the genes <i>tet</i>M, <i>erm</i>B, <i>erm</i>C, and <i>grl</i>A were detected. Fifty-three percent were high biogenic amine (BA) producers, being putrescine the most produced amine. A lowered risk of finding antibiotic-resistant and BA-producing staphylococci is related to some farming methods such as enrolling in a breeding program, use of good farming practices, postdipping teat disinfection, hygienic livestock housing, or periodic check of the milking machine. |
| first_indexed | 2024-03-10T16:25:46Z |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T16:25:46Z |
| publishDate | 2020-09-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-bdd856e0386f4ffabbd913aae847a9512023-11-20T13:17:13ZengMDPI AGAnimals2076-26152020-09-01109162210.3390/ani10091622Farming Practices Influence Antibiotic Resistance and Biogenic Amine Capacity of Staphylococci from Bulk Tank Ewe’s MilkJusta María Poveda0Lorena Jiménez1José Manuel Perea2Ramón Arias3María Llanos Palop4Department of Analytical Chemistry and Food Technology, Regional Institute of Applied Scientific Research (IRICA)/Faculty of Chemical Sciences and Technologies, University of Castilla-La Mancha, Camilo José Cela, 1B, 13071 Ciudad Real, SpainRegional Center of Animal Selection and Reproduction (CERSYRA), Agri-food and Forestry Regional Research and Development Center (IRIAF), JCCM, 13300 Valdepeñas, SpainDepartment of Animal Production, Faculty of Veterinary, University of Cordoba, Campus Rabanales, 14071 Córdoba, SpainRegional Center of Animal Selection and Reproduction (CERSYRA), Agri-food and Forestry Regional Research and Development Center (IRIAF), JCCM, 13300 Valdepeñas, SpainDepartment of Analytical Chemistry and Food Technology, Faculty of Environmental Sciences and Biochemistry, University of Castilla-La Mancha, Avda. Carlos III, s/n, 45071 Toledo, SpainStaphylococci are one of the main microorganisms responsible for intramammary infections in sheep, causing important economic losses for farmers and eventually health problems in humans, especially by the consumption of dairy products made with raw milk containing toxic compounds, such as biogenic amines or antibiotic resistant bacteria. This study aimed to check the presence and safety of staphylococci in bulk tank ewe’s milk from different farms, and to determine the relationship between the presence of these staphylococci and farming practices, by applying nonlinear canonical correlation models (OVERALS). Two-hundred and fifty-nine staphylococci from milk samples from eighteen farms were genotyped and representative isolates of the major clusters were identified as belonging to <i>Staphylococcus (S.) aureus</i>, <i>S. epidermidis</i>, <i>S. arlettae</i>, <i>S. lentus</i>, <i>S. simulans</i>, and <i>S. chromogenes</i> species. Identified isolates were assayed in terms of their safety, by evaluating resistance to antimicrobial drugs and the aminobiogenic capacity, using both phenotypic and genetic assays. Antibiotic resistance phenotypic assay revealed that 82.9% were resistant to some antibiotics, although in the genotypic assay only the genes <i>tet</i>M, <i>erm</i>B, <i>erm</i>C, and <i>grl</i>A were detected. Fifty-three percent were high biogenic amine (BA) producers, being putrescine the most produced amine. A lowered risk of finding antibiotic-resistant and BA-producing staphylococci is related to some farming methods such as enrolling in a breeding program, use of good farming practices, postdipping teat disinfection, hygienic livestock housing, or periodic check of the milking machine.https://www.mdpi.com/2076-2615/10/9/1622staphylococciewe’s milkantibiotic resistancebiogenic aminefarming practices |
| spellingShingle | Justa María Poveda Lorena Jiménez José Manuel Perea Ramón Arias María Llanos Palop Farming Practices Influence Antibiotic Resistance and Biogenic Amine Capacity of Staphylococci from Bulk Tank Ewe’s Milk Animals staphylococci ewe’s milk antibiotic resistance biogenic amine farming practices |
| title | Farming Practices Influence Antibiotic Resistance and Biogenic Amine Capacity of Staphylococci from Bulk Tank Ewe’s Milk |
| title_full | Farming Practices Influence Antibiotic Resistance and Biogenic Amine Capacity of Staphylococci from Bulk Tank Ewe’s Milk |
| title_fullStr | Farming Practices Influence Antibiotic Resistance and Biogenic Amine Capacity of Staphylococci from Bulk Tank Ewe’s Milk |
| title_full_unstemmed | Farming Practices Influence Antibiotic Resistance and Biogenic Amine Capacity of Staphylococci from Bulk Tank Ewe’s Milk |
| title_short | Farming Practices Influence Antibiotic Resistance and Biogenic Amine Capacity of Staphylococci from Bulk Tank Ewe’s Milk |
| title_sort | farming practices influence antibiotic resistance and biogenic amine capacity of staphylococci from bulk tank ewe s milk |
| topic | staphylococci ewe’s milk antibiotic resistance biogenic amine farming practices |
| url | https://www.mdpi.com/2076-2615/10/9/1622 |
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