Red ginseng extract blocks histamine-dependent itch by inhibition of H1R/TRPV1 pathway in sensory neurons
Background: Korean Red Ginseng—a steamed root of Panax ginseng Meyer—has long been used as a traditional medicine in Asian countries. Its antipruritic effect was recently found, but no molecular mechanisms were revealed. Thus, the current study focused on determining the underlying molecular mechani...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-07-01
|
| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S122684531500007X |
| _version_ | 1818190991292628992 |
|---|---|
| author | Yongwoo Jang Wook-Joo Lee Gyu-Sang Hong Won-Sik Shim |
| author_facet | Yongwoo Jang Wook-Joo Lee Gyu-Sang Hong Won-Sik Shim |
| author_sort | Yongwoo Jang |
| collection | DOAJ |
| description | Background: Korean Red Ginseng—a steamed root of Panax ginseng Meyer—has long been used as a traditional medicine in Asian countries. Its antipruritic effect was recently found, but no molecular mechanisms were revealed. Thus, the current study focused on determining the underlying molecular mechanism of Korean Red Ginseng extract (RGE) against histamine-induced itch at the peripheral sensory neuronal level.
Methods: To examine the antipruritic effect of RGE, we performed in vivo scratching behavior test in mice, as well as in vitro calcium imaging and whole-cell patch clamp experiments to elucidate underlying molecular mechanisms.
Results: The results of our in vivo study confirmed that RGE indeed has an antipruritic effect on histamine-induced scratching in mice. In addition, RGE showed a significant inhibitory effect on histamine-induced responses in primary cultures of mouse dorsal root ganglia, suggesting that RGE has a direct inhibitory effect on sensory neuronal level. Results of further experiments showed that RGE inhibits histamine-induced responses on cells expressing both histamine receptor subtype 1 and TRPV1 ion channel, indicating that RGE blocks the histamine receptor type 1/TRPV1 pathway in sensory neurons, which is responsible for histamine-dependent itch sensation.
Conclusion: The current study found for the first time that RGE effectively blocks histamine-induced itch in peripheral sensory neurons. We believe that the current results will provide an insight on itch transmission and will be helpful in understanding how RGE exerts its antipruritic effects. |
| first_indexed | 2024-12-12T00:07:30Z |
| format | Article |
| id | doaj.art-bddafe71e6994d31afbe84fa647fb422 |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-12-12T00:07:30Z |
| publishDate | 2015-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-bddafe71e6994d31afbe84fa647fb4222022-12-22T00:45:04ZengElsevierJournal of Ginseng Research1226-84532015-07-0139325726410.1016/j.jgr.2015.01.004Red ginseng extract blocks histamine-dependent itch by inhibition of H1R/TRPV1 pathway in sensory neuronsYongwoo Jang0Wook-Joo Lee1Gyu-Sang Hong2Won-Sik Shim3Sensory Research Center, Creative Research Initiatives, College of Pharmacy, Seoul National University, Seoul, KoreaCollege of Pharmacy, Gachon University, Incheon, KoreaSensory Research Center, Creative Research Initiatives, College of Pharmacy, Seoul National University, Seoul, KoreaCollege of Pharmacy, Gachon University, Incheon, KoreaBackground: Korean Red Ginseng—a steamed root of Panax ginseng Meyer—has long been used as a traditional medicine in Asian countries. Its antipruritic effect was recently found, but no molecular mechanisms were revealed. Thus, the current study focused on determining the underlying molecular mechanism of Korean Red Ginseng extract (RGE) against histamine-induced itch at the peripheral sensory neuronal level. Methods: To examine the antipruritic effect of RGE, we performed in vivo scratching behavior test in mice, as well as in vitro calcium imaging and whole-cell patch clamp experiments to elucidate underlying molecular mechanisms. Results: The results of our in vivo study confirmed that RGE indeed has an antipruritic effect on histamine-induced scratching in mice. In addition, RGE showed a significant inhibitory effect on histamine-induced responses in primary cultures of mouse dorsal root ganglia, suggesting that RGE has a direct inhibitory effect on sensory neuronal level. Results of further experiments showed that RGE inhibits histamine-induced responses on cells expressing both histamine receptor subtype 1 and TRPV1 ion channel, indicating that RGE blocks the histamine receptor type 1/TRPV1 pathway in sensory neurons, which is responsible for histamine-dependent itch sensation. Conclusion: The current study found for the first time that RGE effectively blocks histamine-induced itch in peripheral sensory neurons. We believe that the current results will provide an insight on itch transmission and will be helpful in understanding how RGE exerts its antipruritic effects.http://www.sciencedirect.com/science/article/pii/S122684531500007XH1RhistamineitchKorean Red Ginseng extractTRPV1 |
| spellingShingle | Yongwoo Jang Wook-Joo Lee Gyu-Sang Hong Won-Sik Shim Red ginseng extract blocks histamine-dependent itch by inhibition of H1R/TRPV1 pathway in sensory neurons Journal of Ginseng Research H1R histamine itch Korean Red Ginseng extract TRPV1 |
| title | Red ginseng extract blocks histamine-dependent itch by inhibition of H1R/TRPV1 pathway in sensory neurons |
| title_full | Red ginseng extract blocks histamine-dependent itch by inhibition of H1R/TRPV1 pathway in sensory neurons |
| title_fullStr | Red ginseng extract blocks histamine-dependent itch by inhibition of H1R/TRPV1 pathway in sensory neurons |
| title_full_unstemmed | Red ginseng extract blocks histamine-dependent itch by inhibition of H1R/TRPV1 pathway in sensory neurons |
| title_short | Red ginseng extract blocks histamine-dependent itch by inhibition of H1R/TRPV1 pathway in sensory neurons |
| title_sort | red ginseng extract blocks histamine dependent itch by inhibition of h1r trpv1 pathway in sensory neurons |
| topic | H1R histamine itch Korean Red Ginseng extract TRPV1 |
| url | http://www.sciencedirect.com/science/article/pii/S122684531500007X |
| work_keys_str_mv | AT yongwoojang redginsengextractblockshistaminedependentitchbyinhibitionofh1rtrpv1pathwayinsensoryneurons AT wookjoolee redginsengextractblockshistaminedependentitchbyinhibitionofh1rtrpv1pathwayinsensoryneurons AT gyusanghong redginsengextractblockshistaminedependentitchbyinhibitionofh1rtrpv1pathwayinsensoryneurons AT wonsikshim redginsengextractblockshistaminedependentitchbyinhibitionofh1rtrpv1pathwayinsensoryneurons |