Serum Tyrosine Level in Acute Murine Toxoplasmosis
Background: Toxoplasmosis is a zoonotic disease caused by the obligate intracellular parasite, Toxoplasma gondii. This global infectious disease has been associated with behavioral changes in rodents and can result in humans' neuropsychiatric symptoms. Since the neurotransmitters alteration can...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-12-01
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| Series: | Iranian Journal of Parasitology |
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| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3183 |
| _version_ | 1818885323390713856 |
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| author | Qasem ASGARI Mina MOUSAEI SISAKHT Shahrbanoo NADERI SHAHABADI Forough KARAMI Mostafa OMIDIAN |
| author_facet | Qasem ASGARI Mina MOUSAEI SISAKHT Shahrbanoo NADERI SHAHABADI Forough KARAMI Mostafa OMIDIAN |
| author_sort | Qasem ASGARI |
| collection | DOAJ |
| description | Background: Toxoplasmosis is a zoonotic disease caused by the obligate intracellular parasite, Toxoplasma gondii. This global infectious disease has been associated with behavioral changes in rodents and can result in humans' neuropsychiatric symptoms. Since the neurotransmitters alteration can cause a behavioral change, in this study, tyrosine level, as a precursor of dopamine, was evaluated in acute murine toxoplasmosis during 2015 and 2016 in Shiraz, Iran.
Methods: At the first, 105 tachyzoites of T. gondii were subcutaneously inoculated to 50 BALB/c mice as experimental groups and 10 mice inoculated by PBS considered as the control group. After that, daily, one group of mice was bled, and sera were collected. Then, their serum tyrosine level was evaluated by HPLC method.
Results: After data analysis, the maximum mean serum tyrosine level was seen at 2th day of post parasite inoculation (0.0194 mg/ ml), with a significant difference compared to the control group (0.0117 mg/ ml, P=0.025). Moreover, the least quantity of serum tyrosine (0.076 mg/ml) was seen on the 5th day, after parasite inoculation, however, no significant difference was seen.
Conclusion: Serum tyrosine level increased in 2 d after inoculation of Toxoplasma, but the level regularly decreased in successive days. Tyrosine level increased by phenylalanine hydroxylase 2 days after inoculation, then tyrosine decreased by tyrosine hydroxylase in the next days. Toxoplasma tyrosine hydroxylase enzymes, at primary days of toxoplasmosis, effect on tyrosine production, and after that, the most effect on tyrosine consumption. |
| first_indexed | 2024-12-19T16:03:37Z |
| format | Article |
| id | doaj.art-bddca3e1f2714d608d658b41885b7edc |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-19T16:03:37Z |
| publishDate | 2020-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-bddca3e1f2714d608d658b41885b7edc2022-12-21T20:14:53ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2020-12-0115410.18502/ijpa.v15i4.4866Serum Tyrosine Level in Acute Murine ToxoplasmosisQasem ASGARI0Mina MOUSAEI SISAKHT1Shahrbanoo NADERI SHAHABADI2Forough KARAMI3Mostafa OMIDIAN4Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranCentral Research Laboratory, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranBackground: Toxoplasmosis is a zoonotic disease caused by the obligate intracellular parasite, Toxoplasma gondii. This global infectious disease has been associated with behavioral changes in rodents and can result in humans' neuropsychiatric symptoms. Since the neurotransmitters alteration can cause a behavioral change, in this study, tyrosine level, as a precursor of dopamine, was evaluated in acute murine toxoplasmosis during 2015 and 2016 in Shiraz, Iran. Methods: At the first, 105 tachyzoites of T. gondii were subcutaneously inoculated to 50 BALB/c mice as experimental groups and 10 mice inoculated by PBS considered as the control group. After that, daily, one group of mice was bled, and sera were collected. Then, their serum tyrosine level was evaluated by HPLC method. Results: After data analysis, the maximum mean serum tyrosine level was seen at 2th day of post parasite inoculation (0.0194 mg/ ml), with a significant difference compared to the control group (0.0117 mg/ ml, P=0.025). Moreover, the least quantity of serum tyrosine (0.076 mg/ml) was seen on the 5th day, after parasite inoculation, however, no significant difference was seen. Conclusion: Serum tyrosine level increased in 2 d after inoculation of Toxoplasma, but the level regularly decreased in successive days. Tyrosine level increased by phenylalanine hydroxylase 2 days after inoculation, then tyrosine decreased by tyrosine hydroxylase in the next days. Toxoplasma tyrosine hydroxylase enzymes, at primary days of toxoplasmosis, effect on tyrosine production, and after that, the most effect on tyrosine consumption.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3183Toxoplasma gondiiTyrosineMiceHigh-performance liquid chromatography |
| spellingShingle | Qasem ASGARI Mina MOUSAEI SISAKHT Shahrbanoo NADERI SHAHABADI Forough KARAMI Mostafa OMIDIAN Serum Tyrosine Level in Acute Murine Toxoplasmosis Iranian Journal of Parasitology Toxoplasma gondii Tyrosine Mice High-performance liquid chromatography |
| title | Serum Tyrosine Level in Acute Murine Toxoplasmosis |
| title_full | Serum Tyrosine Level in Acute Murine Toxoplasmosis |
| title_fullStr | Serum Tyrosine Level in Acute Murine Toxoplasmosis |
| title_full_unstemmed | Serum Tyrosine Level in Acute Murine Toxoplasmosis |
| title_short | Serum Tyrosine Level in Acute Murine Toxoplasmosis |
| title_sort | serum tyrosine level in acute murine toxoplasmosis |
| topic | Toxoplasma gondii Tyrosine Mice High-performance liquid chromatography |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3183 |
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