Unravelling the Distinctive Virulence Traits and Clonal Relationship among the Pseudomonas aeruginosa Isolates from Diabetic Patients
Infections with P. aeruginosa are three times more common in people with diabetes than in non-diabetic individuals. Investigations disclosing the distinguishing traits of P. aeruginosa strains to cause respiratory and wound infection in diabetics is limited. Wound swab and sputum from infected diabe...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2022-09-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/unravelling-the-distinctive-virulence-traits-and-clonal-relationship-among-the-pseudomonas-aeruginosa-isolates-from-diabetic-patients/ |
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| author | Sarika Suresh Kattapuni Suresh Prithvisagar Ballamoole Krishna Kumar Ramya Premanath |
| author_facet | Sarika Suresh Kattapuni Suresh Prithvisagar Ballamoole Krishna Kumar Ramya Premanath |
| author_sort | Sarika Suresh |
| collection | DOAJ |
| description | Infections with P. aeruginosa are three times more common in people with diabetes than in non-diabetic individuals. Investigations disclosing the distinguishing traits of P. aeruginosa strains to cause respiratory and wound infection in diabetics is limited. Wound swab and sputum from infected diabetic patients were used for the isolation of P. aeruginosa. The confirmed isolates were evaluated for their virulence factor production, antibiotic susceptibility, and clonal relationship. The study confirmed the increased virulence of sputum isolates characterized by their multidrug resistant nature, strong biofilm formation at 72h [(p<0.05) =0.003)] and 96h [(p<0.05) =0.002)] and elaboration of proteolytic enzymes (40.0%). Albeit the fact that wound isolates were less virulent than the sputum isolates, there was an increased siderophore production (77.0%). Nearly 90.0% of the isolates including sputum and wound were resistant to colistin. Random Amplified Polymorphic DNA analysis showed no distinct lineages of wound and sputum isolates. The study disclosed the higher prevalence of virulent P. aeruginosa in causing infection in the diabetics. No distinct lineages of the wound and sputum isolates indicated their ability to adapt to different host environments. To the best of our knowledge, this is the first study to show the difference in virulence traits among the P. aeruginosa strains isolated from sputum and wound of diabetic patients. Our study distinctly reveals the significance of periodic examination of antibiotic resistance and virulence factors of P. aeruginosa in order to recognize the possible co-regulatory mechanism involved in their expression. |
| first_indexed | 2024-04-11T10:09:55Z |
| format | Article |
| id | doaj.art-bddd734d0f9f4ab2bc84284333efff58 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-11T10:09:55Z |
| publishDate | 2022-09-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-bddd734d0f9f4ab2bc84284333efff582022-12-22T04:30:07ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2022-09-0116318931908https://doi.org/10.22207/JPAM.16.3.37Unravelling the Distinctive Virulence Traits and Clonal Relationship among the Pseudomonas aeruginosa Isolates from Diabetic PatientsSarika Sureshhttps://orcid.org/0000-0003-3570-8650Kattapuni Suresh Prithvisagarhttps://orcid.org/0000-0002-5333-2071Ballamoole Krishna Kumarhttps://orcid.org/0000-0001-7821-7419Ramya Premanathhttps://orcid.org/0000-0001-5144-8241Infections with P. aeruginosa are three times more common in people with diabetes than in non-diabetic individuals. Investigations disclosing the distinguishing traits of P. aeruginosa strains to cause respiratory and wound infection in diabetics is limited. Wound swab and sputum from infected diabetic patients were used for the isolation of P. aeruginosa. The confirmed isolates were evaluated for their virulence factor production, antibiotic susceptibility, and clonal relationship. The study confirmed the increased virulence of sputum isolates characterized by their multidrug resistant nature, strong biofilm formation at 72h [(p<0.05) =0.003)] and 96h [(p<0.05) =0.002)] and elaboration of proteolytic enzymes (40.0%). Albeit the fact that wound isolates were less virulent than the sputum isolates, there was an increased siderophore production (77.0%). Nearly 90.0% of the isolates including sputum and wound were resistant to colistin. Random Amplified Polymorphic DNA analysis showed no distinct lineages of wound and sputum isolates. The study disclosed the higher prevalence of virulent P. aeruginosa in causing infection in the diabetics. No distinct lineages of the wound and sputum isolates indicated their ability to adapt to different host environments. To the best of our knowledge, this is the first study to show the difference in virulence traits among the P. aeruginosa strains isolated from sputum and wound of diabetic patients. Our study distinctly reveals the significance of periodic examination of antibiotic resistance and virulence factors of P. aeruginosa in order to recognize the possible co-regulatory mechanism involved in their expression.https://microbiologyjournal.org/unravelling-the-distinctive-virulence-traits-and-clonal-relationship-among-the-pseudomonas-aeruginosa-isolates-from-diabetic-patients/pseudomonas aeruginosadiabetesantibiotic resistancevirulence factorsrandom amplified polymorphic dna analysis |
| spellingShingle | Sarika Suresh Kattapuni Suresh Prithvisagar Ballamoole Krishna Kumar Ramya Premanath Unravelling the Distinctive Virulence Traits and Clonal Relationship among the Pseudomonas aeruginosa Isolates from Diabetic Patients Journal of Pure and Applied Microbiology pseudomonas aeruginosa diabetes antibiotic resistance virulence factors random amplified polymorphic dna analysis |
| title | Unravelling the Distinctive Virulence Traits and Clonal Relationship among the Pseudomonas aeruginosa Isolates from Diabetic Patients |
| title_full | Unravelling the Distinctive Virulence Traits and Clonal Relationship among the Pseudomonas aeruginosa Isolates from Diabetic Patients |
| title_fullStr | Unravelling the Distinctive Virulence Traits and Clonal Relationship among the Pseudomonas aeruginosa Isolates from Diabetic Patients |
| title_full_unstemmed | Unravelling the Distinctive Virulence Traits and Clonal Relationship among the Pseudomonas aeruginosa Isolates from Diabetic Patients |
| title_short | Unravelling the Distinctive Virulence Traits and Clonal Relationship among the Pseudomonas aeruginosa Isolates from Diabetic Patients |
| title_sort | unravelling the distinctive virulence traits and clonal relationship among the pseudomonas aeruginosa isolates from diabetic patients |
| topic | pseudomonas aeruginosa diabetes antibiotic resistance virulence factors random amplified polymorphic dna analysis |
| url | https://microbiologyjournal.org/unravelling-the-distinctive-virulence-traits-and-clonal-relationship-among-the-pseudomonas-aeruginosa-isolates-from-diabetic-patients/ |
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