Microfluidics for the rapid detection of Escherichia coli O157:H7 using antibody-coated microspheres

Microfluidics for the rapid detection of Escherichia coli O157:H7 using antibody-coated microspheres

This study developed a novel method for the rapid detection of Escherichia coli (E. coli) O157:H7 on a microfluidic platform. First, the concentration of bacteria in a sample was determined with the adenosine triphosphate (ATP) method. Then, the specific detection of E. coli was achieved in a microf...

Full description

Bibliographic Details
Main Authors:	Bo Song, Jiayuan Yu, Yan Sun, Qiao Wang, Shengnan Xu, Yichen Jia, Shuying Lin, Yueying Zhang, Chen Wang, Yingbo Zhang, Xiaojie Zhang
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2021-01-01
Series:	Bioengineered
Subjects:	microfluidic chip detection of ehec o157: h7 atp microspheres
Online Access:	http://dx.doi.org/10.1080/21655979.2020.1870805

Similar Items

Persistent Circulation of Enterohemorrhagic Escherichia coli (EHEC) O157:H7 in Cattle Farms: Characterization of Enterohemorrhagic Escherichia coli O157:H7 Strains and Fecal Microbial Communities of Bovine Shedders and Non-shedders
by: Delphine Bibbal, et al.
Published: (2022-03-01)

Phylogenetic Analysis of Enterohemorrhagic Escherichia coli O157, Germany, 1987–2008
by: Christian Jenke, et al.
Published: (2010-04-01)

Advanced microfluidic devices for fabricating multi‐structural hydrogel microsphere
by: Zehao Chen, et al.
Published: (2021-12-01)

Identification of Intermediate in Evolutionary Model of Enterohemorrhagic Escherichia coli O157
by: Christian Jenke, et al.
Published: (2012-04-01)

Revisiting the STEC testing approach: using espK and espV to make enterohemorrhagic Escherichia coli (EHEC) detection more reliable in beef
by: Sabine eDelannoy, et al.
Published: (2016-01-01)

The Fimbrial Gene z3276 in Enterohemorrhagic Escherichia coli O157:H7 Contributes to Bacterial Pathogenicity
by: Bicheng Zhang, et al.
Published: (2018-07-01)

Multiplex polymerase chain reaction typing scheme based on Escherichia coli O157:H7 Sakai prophage (Sp)-associated genes
by: István Tóth, et al.
Published: (2022-07-01)

Application of a novel lytic phage vB_EcoM_SQ17 for the biocontrol of Enterohemorrhagic Escherichia coli O157:H7 and Enterotoxigenic E. coli in food matrices
by: Yan Zhou, et al.
Published: (2022-08-01)

Microfluidic-based fabrication and characterization of drug-loaded PLGA magnetic microspheres with tunable shell thickness
by: Chunpeng He, et al.
Published: (2021-01-01)

Factors Involved in the Persistence of a Shiga Toxin-Producing Escherichia coli O157:H7 Strain in Bovine Feces and Gastro-Intestinal Content
by: Audrey Segura, et al.
Published: (2018-03-01)

The Role of <i>Escherichia coli</i> Shiga Toxins in STEC Colonization of Cattle
by: Christian Menge
Published: (2020-09-01)

Molecular Biology of <em>Escherichia coli</em> Shiga Toxins’ Effects on Mammalian Cells
by: Christian Menge
Published: (2020-05-01)

Expression and Functional Characterization of Various Chaperon-Usher Fimbriae, Curli Fimbriae, and Type 4 Pili of Enterohemorrhagic Escherichia coli O157:H7 Sakai
by: Laura Elpers, et al.
Published: (2020-03-01)

Microfluidic Production of Autofluorescent BSA Hydrogel Microspheres and Their Sequential Trapping for Fluorescence-Based On-Chip Permanganate Sensing
by: Linbo Liu, et al.
Published: (2020-10-01)

Regulation of flagellar motility and biosynthesis in enterohemorrhagic Escherichia coli O157:H7
by: Hongmin Sun, et al.
Published: (2022-12-01)

Thermal and ignition properties of hexanitrostilbene (HNS) microspheres prepared by droplet microfluidics
by: Rui-shan Han, et al.
Published: (2023-07-01)

Zebrafish (<named-content content-type="genus-species">Danio rerio</named-content>) as a Vertebrate Model Host To Study Colonization, Pathogenesis, and Transmission of Foodborne <named-content content-type="genus-species">Escherichia coli</named-content> O157
by: Daniel H. Stones, et al.
Published: (2017-10-01)

Rapid and Specific Detection of Escherichia coli O157:H7 in Ground Beef Using Immunomagnetic Separation Combined with Loop-Mediated Isothermal Amplification
by: Qin Yu, et al.
Published: (2018-06-01)

Precise Fabrication of Porous Microspheres by Iso-Density Emulsion Combined with Microfluidics
by: Yuxiao Shi, et al.
Published: (2022-06-01)

Quantitative generation of microfluidic flow by using optically driven microspheres
by: Songyu Hu, et al.
Published: (2019-07-01)

Transcriptomic analysis reveals specific metabolic pathways of enterohemorrhagic Escherichia coli O157:H7 in bovine digestive contents
by: Audrey Segura, et al.
Published: (2018-10-01)

A Novel pH-Regulated, Unusual 603 bp Overlapping Protein Coding Gene pop Is Encoded Antisense to ompA in Escherichia coli O157:H7 (EHEC)
by: Barbara Zehentner, et al.
Published: (2020-03-01)

<i>Pediococcus pentosaceus</i> IM96 Exerts Protective Effects against Enterohemorrhagic <i>Escherichia coli</i> O157:H7 Infection In Vivo
by: Haixin Li, et al.
Published: (2021-11-01)

Three different routes of EHEC O157:H7 infection were used to establish EHEC broiler model
by: Yan-Na Guo, et al.
Published: (2024-04-01)

Three Novel Antisense Overlapping Genes in E. coli O157:H7 EDL933
by: Franziska Graf, et al.
Published: (2023-02-01)

Facile Microfluidic Fabrication of Biocompatible Hydrogel Microspheres in a Novel Microfluidic Device
by: Minjun Chen, et al.
Published: (2022-06-01)

Effect of Eccentricity Difference on the Mechanical Response of Microfluidics-Derived Hollow Silica Microspheres during Nanoindentation
by: Hao Wu, et al.
Published: (2024-01-01)

Development of In Situ Microfluidic System for Preparation of Controlled Porous Microsphere for Tissue Engineering
by: Ji Hwan Han, et al.
Published: (2022-10-01)

3D Printed Reconfigurable Modular Microfluidic System for Generating Gel Microspheres
by: Xiaojun Chen, et al.
Published: (2020-02-01)

3D Printed Microfluidic Devices for Drug Release Assays
by: Benzion Amoyav, et al.
Published: (2020-12-01)

On-Chip Facile Preparation of Monodisperse Resorcinol Formaldehyde (RF) Resin Microspheres
by: Jianmei Wang, et al.
Published: (2018-01-01)

512-Channel Geometric Droplet-Splitting Microfluidic Device by Injection of Premixed Emulsion for Microsphere Production
by: Chul Min Kim, et al.
Published: (2020-04-01)

Effect of Carvacrol on O157 and non-O157 Shiga Toxin Producing Escherichia coli
by: Alexandros Stratakos, et al.
Published: (2023-09-01)

Microfluidic Preparation of pH-Responsive Microsphere Fibers and Their Controlled Drug Release Properties
by: Ning Wang, et al.
Published: (2023-12-01)

Point-of-Care Diagnostic Devices for Detection of <i>Escherichia coli</i> O157:H7 Using Microfluidic Systems: A Focused Review
by: Naseem Abbas, et al.
Published: (2023-07-01)

Pathogenomes of Atypical Non-shigatoxigenic Escherichia coli NSF/SF O157:H7/NM: Comprehensive Phylogenomic Analysis Using Closed Genomes
by: Emmanuel C. Nyong, et al.
Published: (2020-04-01)

Microfabrication of Nonplanar Polymeric Microfluidics
by: Pin-Chuan Chen, et al.
Published: (2018-09-01)

A Novel Role of the Two-Component System Response Regulator UvrY in Enterohemorrhagic <i>Escherichia coli</i> O157:H7 Pathogenicity Regulation
by: Pan Wu, et al.
Published: (2023-01-01)

Microencapsulation of growth factors by microfluidic system
by: Lilith M. Caballero-Aguilar, et al.
Published: (2021-01-01)

Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats inJordanwith evidence of multiresistant serotype O157:H7
by: R. Novotna, et al.
Published: (2005-03-01)