Synergistic influence on microbial communities ascribed to copper and tetracycline during aerobic composting: Insights into bacterial and fungal structures
There are a considerable number of discussions aimed at analyzing microbial communities and their functions during the composting process. However, microbial succession under copper (Cu) and tetracycline (TCH)-stressed conditions has received less attention. Thus, this work analyzed the bacterial an...
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| Format: | Article |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Ecology and Evolution |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fevo.2022.1019494/full |
| _version_ | 1828728393752903680 |
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| author | Hu Cui Hu Cui Lixia Wang Lixia Wang Jian Zhang |
| author_facet | Hu Cui Hu Cui Lixia Wang Lixia Wang Jian Zhang |
| author_sort | Hu Cui |
| collection | DOAJ |
| description | There are a considerable number of discussions aimed at analyzing microbial communities and their functions during the composting process. However, microbial succession under copper (Cu) and tetracycline (TCH)-stressed conditions has received less attention. Thus, this work analyzed the bacterial and fungal structures with high-throughput sequencing in Cu/TCH-amended composting (Cu: 0, 100, and 500 mg/kg; TCH: 0, 50, and 300 mg/kg), and the dominating controls on microbial diversity were identified using redundancy analysis (RDA) and structural equation models (SEMs). Low-concentration Cu increased the peak temperature (57°C) at the thermophilic phase. Composting phase-derived changes in bacterial and fungal communities were significant, while Cu and TCH showed a remarkable influence on fungi but not on bacteria. Cu and TCH inhibited Firmicutes' activity while promoting Actinobacteriota growth. Low-concentration Cu and TCH had a negative effect on Basidiomycota in the thermophilic phase and a positive influence on Chytridiomycota in the mature phase. TOC and TN were primary controls on the changes in microbial communities. NH4+-N and NO3--N were more beneficial to fungi with a contribution proportion of 42.13 and 16.85%, respectively. These findings could provide theoretical guidance for the directional research on microbial inoculants. |
| first_indexed | 2024-04-12T14:15:43Z |
| format | Article |
| id | doaj.art-bde06f2c695a4f00b90c8c2168d580c8 |
| institution | Directory Open Access Journal |
| issn | 2296-701X |
| language | English |
| last_indexed | 2024-04-12T14:15:43Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Ecology and Evolution |
| spelling | doaj.art-bde06f2c695a4f00b90c8c2168d580c82022-12-22T03:29:45ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2022-11-011010.3389/fevo.2022.10194941019494Synergistic influence on microbial communities ascribed to copper and tetracycline during aerobic composting: Insights into bacterial and fungal structuresHu Cui0Hu Cui1Lixia Wang2Lixia Wang3Jian Zhang4Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaJilin Provincial Engineering Center of CWs Design in Cold Region and Beautiful Country Construction, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaKey Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaJilin Provincial Engineering Center of CWs Design in Cold Region and Beautiful Country Construction, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaThe School of Sciences, Qiqihar University, Qiqihar, ChinaThere are a considerable number of discussions aimed at analyzing microbial communities and their functions during the composting process. However, microbial succession under copper (Cu) and tetracycline (TCH)-stressed conditions has received less attention. Thus, this work analyzed the bacterial and fungal structures with high-throughput sequencing in Cu/TCH-amended composting (Cu: 0, 100, and 500 mg/kg; TCH: 0, 50, and 300 mg/kg), and the dominating controls on microbial diversity were identified using redundancy analysis (RDA) and structural equation models (SEMs). Low-concentration Cu increased the peak temperature (57°C) at the thermophilic phase. Composting phase-derived changes in bacterial and fungal communities were significant, while Cu and TCH showed a remarkable influence on fungi but not on bacteria. Cu and TCH inhibited Firmicutes' activity while promoting Actinobacteriota growth. Low-concentration Cu and TCH had a negative effect on Basidiomycota in the thermophilic phase and a positive influence on Chytridiomycota in the mature phase. TOC and TN were primary controls on the changes in microbial communities. NH4+-N and NO3--N were more beneficial to fungi with a contribution proportion of 42.13 and 16.85%, respectively. These findings could provide theoretical guidance for the directional research on microbial inoculants.https://www.frontiersin.org/articles/10.3389/fevo.2022.1019494/fullco-compostingcoppertetracycline hydrochloridebacteriafungi |
| spellingShingle | Hu Cui Hu Cui Lixia Wang Lixia Wang Jian Zhang Synergistic influence on microbial communities ascribed to copper and tetracycline during aerobic composting: Insights into bacterial and fungal structures Frontiers in Ecology and Evolution co-composting copper tetracycline hydrochloride bacteria fungi |
| title | Synergistic influence on microbial communities ascribed to copper and tetracycline during aerobic composting: Insights into bacterial and fungal structures |
| title_full | Synergistic influence on microbial communities ascribed to copper and tetracycline during aerobic composting: Insights into bacterial and fungal structures |
| title_fullStr | Synergistic influence on microbial communities ascribed to copper and tetracycline during aerobic composting: Insights into bacterial and fungal structures |
| title_full_unstemmed | Synergistic influence on microbial communities ascribed to copper and tetracycline during aerobic composting: Insights into bacterial and fungal structures |
| title_short | Synergistic influence on microbial communities ascribed to copper and tetracycline during aerobic composting: Insights into bacterial and fungal structures |
| title_sort | synergistic influence on microbial communities ascribed to copper and tetracycline during aerobic composting insights into bacterial and fungal structures |
| topic | co-composting copper tetracycline hydrochloride bacteria fungi |
| url | https://www.frontiersin.org/articles/10.3389/fevo.2022.1019494/full |
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