Susceptibility Profile of Candida albicans Biofilms on Polyvinyl Chloride Endotracheal Tube to Antifungal Azoles
One of the most prevalent causative organisms in human fungal infections is Candida albicans. The extensive utility of medical devices such as endotracheal tube has resulted in the increased cases of fungal infections. The present study visualized Candida albicans biofilm on polyvinyl chloride endot...
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| Format: | Article |
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Journal of Pure and Applied Microbiology
2020-06-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/susceptibility-profile-of-candida-albicans-biofilms-on-polyvinyl-chloride-endotracheal-tube-to-antifungal-azoles/ |
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| author | Derick Erl P. Sumalapao Frederico Martin D. Alegre Pauline Bridgette D. Salazar Nelson R. Villarante Isidro C. Sia Nina G. Gloriani |
| author_facet | Derick Erl P. Sumalapao Frederico Martin D. Alegre Pauline Bridgette D. Salazar Nelson R. Villarante Isidro C. Sia Nina G. Gloriani |
| author_sort | Derick Erl P. Sumalapao |
| collection | DOAJ |
| description | One of the most prevalent causative organisms in human fungal infections is Candida albicans. The extensive utility of medical devices such as endotracheal tube has resulted in the increased cases of fungal infections. The present study visualized Candida albicans biofilm on polyvinyl chloride endotracheal tube and described the susceptibility profile of the biofilm to antifungal azoles. Biofilm was examined using light and fluorescence microscopy. The susceptibility profile of planktonic and sessile cells with posaconazole, miconazole, voriconazole, and fluconazole was assessed employing the standard disc diffusion method. Planktonic C. albicans was susceptible to the identified antifungal azoles, whereas the sessile cells had varying susceptibility profiles. The biofilms remained susceptible to voriconazole and fluconazole, susceptible-dose dependent to posaconazole, and resistant to miconazole. Findings of the present study can provide relevant information on the resistance mechanism of fungal biofilms with azoles and in the design of new pharmacologic interventions intended as treatment of mycoses. |
| first_indexed | 2024-04-11T15:06:27Z |
| format | Article |
| id | doaj.art-bde119e9763842728ccdf5bd31342592 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-11T15:06:27Z |
| publishDate | 2020-06-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-bde119e9763842728ccdf5bd313425922022-12-22T04:16:48ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2020-06-011421143114810.22207/JPAM.14.2.09Susceptibility Profile of Candida albicans Biofilms on Polyvinyl Chloride Endotracheal Tube to Antifungal AzolesDerick Erl P. Sumalapao0https://orcid.org/0000-0003-0361-8853Frederico Martin D. Alegre1https://orcid.org/0000-0001-7389-8534Pauline Bridgette D. Salazar2https://orcid.org/0000-0001-9048-1431Nelson R. Villarante3https://orcid.org/0000-0003-3878-3509Isidro C. Sia4https://orcid.org/0000-0003-3543-3749Nina G. Gloriani5https://orcid.org/0000-0001-9275-5758Department of Epidemiology and Biostatistics, College of Public Health, University of the Philippines Manila, Manila, Philippines. Biology Department, College of Science, De La Salle University, Manila, Philippines.Biology Department, College of Science, De La Salle University, Manila, Philippines. College of Medicine, De La Salle Medical and Health Sciences Institute, Cavite, Philippines. Biology Department, College of Science, De La Salle University, Manila, Philippines. College of Medicine, De La Salle Medical and Health Sciences Institute, Cavite, Philippines. Department of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Manila, Manila, Philippines.Department of Pharmacology and Toxicology, College of Medicine, University of the Philippines Manila, Manila, Philippines. Integrative Medicine for Alternative Healthcare Systems Philippines, Inc., Quezon City, Philippines.Department of Medical Microbiology, College of Public Health, University of the Philippines Manila, Manila, Philippines.One of the most prevalent causative organisms in human fungal infections is Candida albicans. The extensive utility of medical devices such as endotracheal tube has resulted in the increased cases of fungal infections. The present study visualized Candida albicans biofilm on polyvinyl chloride endotracheal tube and described the susceptibility profile of the biofilm to antifungal azoles. Biofilm was examined using light and fluorescence microscopy. The susceptibility profile of planktonic and sessile cells with posaconazole, miconazole, voriconazole, and fluconazole was assessed employing the standard disc diffusion method. Planktonic C. albicans was susceptible to the identified antifungal azoles, whereas the sessile cells had varying susceptibility profiles. The biofilms remained susceptible to voriconazole and fluconazole, susceptible-dose dependent to posaconazole, and resistant to miconazole. Findings of the present study can provide relevant information on the resistance mechanism of fungal biofilms with azoles and in the design of new pharmacologic interventions intended as treatment of mycoses.https://microbiologyjournal.org/susceptibility-profile-of-candida-albicans-biofilms-on-polyvinyl-chloride-endotracheal-tube-to-antifungal-azoles/fluconazolefungal infectionsmiconazolepolymer-based materialposaconazolestandard disc diffusion methodvoriconazole |
| spellingShingle | Derick Erl P. Sumalapao Frederico Martin D. Alegre Pauline Bridgette D. Salazar Nelson R. Villarante Isidro C. Sia Nina G. Gloriani Susceptibility Profile of Candida albicans Biofilms on Polyvinyl Chloride Endotracheal Tube to Antifungal Azoles Journal of Pure and Applied Microbiology fluconazole fungal infections miconazole polymer-based material posaconazole standard disc diffusion method voriconazole |
| title | Susceptibility Profile of Candida albicans Biofilms on Polyvinyl Chloride Endotracheal Tube to Antifungal Azoles |
| title_full | Susceptibility Profile of Candida albicans Biofilms on Polyvinyl Chloride Endotracheal Tube to Antifungal Azoles |
| title_fullStr | Susceptibility Profile of Candida albicans Biofilms on Polyvinyl Chloride Endotracheal Tube to Antifungal Azoles |
| title_full_unstemmed | Susceptibility Profile of Candida albicans Biofilms on Polyvinyl Chloride Endotracheal Tube to Antifungal Azoles |
| title_short | Susceptibility Profile of Candida albicans Biofilms on Polyvinyl Chloride Endotracheal Tube to Antifungal Azoles |
| title_sort | susceptibility profile of candida albicans biofilms on polyvinyl chloride endotracheal tube to antifungal azoles |
| topic | fluconazole fungal infections miconazole polymer-based material posaconazole standard disc diffusion method voriconazole |
| url | https://microbiologyjournal.org/susceptibility-profile-of-candida-albicans-biofilms-on-polyvinyl-chloride-endotracheal-tube-to-antifungal-azoles/ |
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