Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage
IntroductionFreeze-drying techniques give alternative preservation mammalian spermatozoa without liquid nitrogen. However, most of the work has been conducted in the laboratory mouse, while little information has been gathered on large animals that could also benefit from this kind of storage.Method...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1270266/full |
| _version_ | 1797435538923323392 |
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| author | Luca Palazzese Federica Turri Debora Agata Anzalone Joseph Saragusty Jacques Bonnet Jacques Bonnet Marthe Colotte Sophie Tuffet Flavia Pizzi Alessia Luciani Kazutsugu Matsukawa Marta Czernik Marta Czernik Pasqualino Loi |
| author_facet | Luca Palazzese Federica Turri Debora Agata Anzalone Joseph Saragusty Jacques Bonnet Jacques Bonnet Marthe Colotte Sophie Tuffet Flavia Pizzi Alessia Luciani Kazutsugu Matsukawa Marta Czernik Marta Czernik Pasqualino Loi |
| author_sort | Luca Palazzese |
| collection | DOAJ |
| description | IntroductionFreeze-drying techniques give alternative preservation mammalian spermatozoa without liquid nitrogen. However, most of the work has been conducted in the laboratory mouse, while little information has been gathered on large animals that could also benefit from this kind of storage.MethodsThis work adapted a technique known as vacuum-drying encapsulation (VDE), originally developed for nucleic acid conservation in anhydrous state, to ram spermatozoa, and compared it to canonical lyophilization (FD), testing long-term storage at room temperature (RT) and 4°C.Results and discussionThe results demonstrated better structural stability, namely lipid composition and DNA integrity, in VDE spermatozoa than FD ones, with outcomes at RT storage comparable to 4°C. Likewise, in VDE the embryonic development was higher than in FD samples (12.8% vs. 8.7%, p < 0.001, respectively). Our findings indicated that in large mammals, it is important to consider dehydration-related changes in sperm polyunsaturated fatty acids coupled with DNA alterations, given their crucial role in embryonic development. |
| first_indexed | 2024-03-09T10:49:18Z |
| format | Article |
| id | doaj.art-bde17db0ca8b46ffadc516e96cc6eb8a |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-09T10:49:18Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-bde17db0ca8b46ffadc516e96cc6eb8a2023-12-01T09:36:49ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-11-011010.3389/fvets.2023.12702661270266Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storageLuca Palazzese0Federica Turri1Debora Agata Anzalone2Joseph Saragusty3Jacques Bonnet4Jacques Bonnet5Marthe Colotte6Sophie Tuffet7Flavia Pizzi8Alessia Luciani9Kazutsugu Matsukawa10Marta Czernik11Marta Czernik12Pasqualino Loi13Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Warsaw, PolandInstitute of Agricultural Biology and Biotechnology (IBBA), National Research Council (CNR), Lodi, ItalyDepartment of Veterinary Medicine, University of Teramo, Teramo, ItalyDepartment of Veterinary Medicine, University of Teramo, Teramo, ItalyLaboratoire de Recherche et Développement, Imagene Company, Pessac, FranceInstitut Bergonié, INSERM, Université de Bordeaux, Bordeaux, FrancePlateforme de Production, Imagene, Genopole, Evry, FrancePlateforme de Production, Imagene, Genopole, Evry, FranceInstitute of Agricultural Biology and Biotechnology (IBBA), National Research Council (CNR), Lodi, ItalyDepartment of Veterinary Medicine, University of Teramo, Teramo, ItalyFaculty of Agriculture and Marine Science, Kochi University, Kochi, JapanInstitute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Warsaw, PolandDepartment of Veterinary Medicine, University of Teramo, Teramo, ItalyDepartment of Veterinary Medicine, University of Teramo, Teramo, ItalyIntroductionFreeze-drying techniques give alternative preservation mammalian spermatozoa without liquid nitrogen. However, most of the work has been conducted in the laboratory mouse, while little information has been gathered on large animals that could also benefit from this kind of storage.MethodsThis work adapted a technique known as vacuum-drying encapsulation (VDE), originally developed for nucleic acid conservation in anhydrous state, to ram spermatozoa, and compared it to canonical lyophilization (FD), testing long-term storage at room temperature (RT) and 4°C.Results and discussionThe results demonstrated better structural stability, namely lipid composition and DNA integrity, in VDE spermatozoa than FD ones, with outcomes at RT storage comparable to 4°C. Likewise, in VDE the embryonic development was higher than in FD samples (12.8% vs. 8.7%, p < 0.001, respectively). Our findings indicated that in large mammals, it is important to consider dehydration-related changes in sperm polyunsaturated fatty acids coupled with DNA alterations, given their crucial role in embryonic development.https://www.frontiersin.org/articles/10.3389/fvets.2023.1270266/fullencapsulationlipidomicspolyunsaturated fatty acidsICSIovine |
| spellingShingle | Luca Palazzese Federica Turri Debora Agata Anzalone Joseph Saragusty Jacques Bonnet Jacques Bonnet Marthe Colotte Sophie Tuffet Flavia Pizzi Alessia Luciani Kazutsugu Matsukawa Marta Czernik Marta Czernik Pasqualino Loi Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage Frontiers in Veterinary Science encapsulation lipidomics polyunsaturated fatty acids ICSI ovine |
| title | Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage |
| title_full | Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage |
| title_fullStr | Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage |
| title_full_unstemmed | Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage |
| title_short | Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage |
| title_sort | reviving vacuum dried encapsulated ram spermatozoa via icsi after 2 years of storage |
| topic | encapsulation lipidomics polyunsaturated fatty acids ICSI ovine |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1270266/full |
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