In vitro Induced Mitotic Polyploidy in Drosera capensis L.

The objective of this study was to induce mitotic polyploidization in Drosera capensis. Tetraploid plants of D. capensis were induced successfully by treating leaf segments in vitro with oryzalin solution with four different concentrations (20, 40, 60 or 80 μM) for 12, 24 or 48 hours. Three tetraplo...

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Main Authors: Zahumenicka Pavla, Sysova Barbora, Holik Ales, Fernandez Eloy C.
Format: Article
Language:English
Published: Sciendo 2013-12-01
Series:Agricultura Tropica et Subtropica
Subjects:
Online Access:https://doi.org/10.2478/ats-2013-0020
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author Zahumenicka Pavla
Sysova Barbora
Holik Ales
Fernandez Eloy C.
author_facet Zahumenicka Pavla
Sysova Barbora
Holik Ales
Fernandez Eloy C.
author_sort Zahumenicka Pavla
collection DOAJ
description The objective of this study was to induce mitotic polyploidization in Drosera capensis. Tetraploid plants of D. capensis were induced successfully by treating leaf segments in vitro with oryzalin solution with four different concentrations (20, 40, 60 or 80 μM) for 12, 24 or 48 hours. Three tetraploid (2n = 4x = 80) plants were obtained in three treatments (20 μM for 48 h, 60 μM for 24 h and 80 μM for 12 h). Tetraploidy was confirmed by flow cytometry. The survival rate of these plants was not significantly influenced by oryzalin concentration or exposure time.
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spelling doaj.art-bde26c7fd2074b5695857c1ecaa9ca2a2023-05-29T10:54:38ZengSciendoAgricultura Tropica et Subtropica1801-05712013-12-0146410711010.2478/ats-2013-0020In vitro Induced Mitotic Polyploidy in Drosera capensis L.Zahumenicka Pavla0Sysova Barbora1Holik Ales2Fernandez Eloy C.3Department of Crop Sciences and Agroforestry in Tropics and Subtropics, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Czech RepublicDepartment of Crop Sciences and Agroforestry in Tropics and Subtropics, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Czech RepublicDepartment of Horticulture, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Czech RepublicDepartment of Crop Sciences and Agroforestry in Tropics and Subtropics, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Czech RepublicThe objective of this study was to induce mitotic polyploidization in Drosera capensis. Tetraploid plants of D. capensis were induced successfully by treating leaf segments in vitro with oryzalin solution with four different concentrations (20, 40, 60 or 80 μM) for 12, 24 or 48 hours. Three tetraploid (2n = 4x = 80) plants were obtained in three treatments (20 μM for 48 h, 60 μM for 24 h and 80 μM for 12 h). Tetraploidy was confirmed by flow cytometry. The survival rate of these plants was not significantly influenced by oryzalin concentration or exposure time.https://doi.org/10.2478/ats-2013-0020drosera capensis l.droseraceaeflow cytometryoryzalinpolyploidization
spellingShingle Zahumenicka Pavla
Sysova Barbora
Holik Ales
Fernandez Eloy C.
In vitro Induced Mitotic Polyploidy in Drosera capensis L.
Agricultura Tropica et Subtropica
drosera capensis l.
droseraceae
flow cytometry
oryzalin
polyploidization
title In vitro Induced Mitotic Polyploidy in Drosera capensis L.
title_full In vitro Induced Mitotic Polyploidy in Drosera capensis L.
title_fullStr In vitro Induced Mitotic Polyploidy in Drosera capensis L.
title_full_unstemmed In vitro Induced Mitotic Polyploidy in Drosera capensis L.
title_short In vitro Induced Mitotic Polyploidy in Drosera capensis L.
title_sort in vitro induced mitotic polyploidy in drosera capensis l
topic drosera capensis l.
droseraceae
flow cytometry
oryzalin
polyploidization
url https://doi.org/10.2478/ats-2013-0020
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AT sysovabarbora invitroinducedmitoticpolyploidyindroseracapensisl
AT holikales invitroinducedmitoticpolyploidyindroseracapensisl
AT fernandezeloyc invitroinducedmitoticpolyploidyindroseracapensisl