In vitro Induced Mitotic Polyploidy in Drosera capensis L.
The objective of this study was to induce mitotic polyploidization in Drosera capensis. Tetraploid plants of D. capensis were induced successfully by treating leaf segments in vitro with oryzalin solution with four different concentrations (20, 40, 60 or 80 μM) for 12, 24 or 48 hours. Three tetraplo...
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Language: | English |
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Sciendo
2013-12-01
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Series: | Agricultura Tropica et Subtropica |
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Online Access: | https://doi.org/10.2478/ats-2013-0020 |
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author | Zahumenicka Pavla Sysova Barbora Holik Ales Fernandez Eloy C. |
author_facet | Zahumenicka Pavla Sysova Barbora Holik Ales Fernandez Eloy C. |
author_sort | Zahumenicka Pavla |
collection | DOAJ |
description | The objective of this study was to induce mitotic polyploidization in Drosera capensis. Tetraploid plants of D. capensis were induced successfully by treating leaf segments in vitro with oryzalin solution with four different concentrations (20, 40, 60 or 80 μM) for 12, 24 or 48 hours. Three tetraploid (2n = 4x = 80) plants were obtained in three treatments (20 μM for 48 h, 60 μM for 24 h and 80 μM for 12 h). Tetraploidy was confirmed by flow cytometry. The survival rate of these plants was not significantly influenced by oryzalin concentration or exposure time. |
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format | Article |
id | doaj.art-bde26c7fd2074b5695857c1ecaa9ca2a |
institution | Directory Open Access Journal |
issn | 1801-0571 |
language | English |
last_indexed | 2024-03-13T08:52:35Z |
publishDate | 2013-12-01 |
publisher | Sciendo |
record_format | Article |
series | Agricultura Tropica et Subtropica |
spelling | doaj.art-bde26c7fd2074b5695857c1ecaa9ca2a2023-05-29T10:54:38ZengSciendoAgricultura Tropica et Subtropica1801-05712013-12-0146410711010.2478/ats-2013-0020In vitro Induced Mitotic Polyploidy in Drosera capensis L.Zahumenicka Pavla0Sysova Barbora1Holik Ales2Fernandez Eloy C.3Department of Crop Sciences and Agroforestry in Tropics and Subtropics, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Czech RepublicDepartment of Crop Sciences and Agroforestry in Tropics and Subtropics, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Czech RepublicDepartment of Horticulture, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Czech RepublicDepartment of Crop Sciences and Agroforestry in Tropics and Subtropics, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Czech RepublicThe objective of this study was to induce mitotic polyploidization in Drosera capensis. Tetraploid plants of D. capensis were induced successfully by treating leaf segments in vitro with oryzalin solution with four different concentrations (20, 40, 60 or 80 μM) for 12, 24 or 48 hours. Three tetraploid (2n = 4x = 80) plants were obtained in three treatments (20 μM for 48 h, 60 μM for 24 h and 80 μM for 12 h). Tetraploidy was confirmed by flow cytometry. The survival rate of these plants was not significantly influenced by oryzalin concentration or exposure time.https://doi.org/10.2478/ats-2013-0020drosera capensis l.droseraceaeflow cytometryoryzalinpolyploidization |
spellingShingle | Zahumenicka Pavla Sysova Barbora Holik Ales Fernandez Eloy C. In vitro Induced Mitotic Polyploidy in Drosera capensis L. Agricultura Tropica et Subtropica drosera capensis l. droseraceae flow cytometry oryzalin polyploidization |
title | In vitro Induced Mitotic Polyploidy in Drosera capensis L. |
title_full | In vitro Induced Mitotic Polyploidy in Drosera capensis L. |
title_fullStr | In vitro Induced Mitotic Polyploidy in Drosera capensis L. |
title_full_unstemmed | In vitro Induced Mitotic Polyploidy in Drosera capensis L. |
title_short | In vitro Induced Mitotic Polyploidy in Drosera capensis L. |
title_sort | in vitro induced mitotic polyploidy in drosera capensis l |
topic | drosera capensis l. droseraceae flow cytometry oryzalin polyploidization |
url | https://doi.org/10.2478/ats-2013-0020 |
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