Efficacy of 5% Aminolaevulinic Acid and Red Light on <i>Enterococcus faecalis</i> in Infected Root Canals
Background: In this ex vivo study, the aim was to evaluate the effects of ALAD and red light on <i>Enterococcus faecalis</i> in infected root canals using a special intracanal fiber. Methods: A total of 70 extracted, single-rooted teeth were used. The teeth were decoronated at the length...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/9/2/125 |
| _version_ | 1797620906614325248 |
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| author | Teocrito Carlesi Tatiane Cristina Dotta Tania Vanessa Pierfelice Emira D’Amico Stefania Lepore Domenico Tripodi Adriano Piattelli Simonetta D’Ercole Morena Petrini |
| author_facet | Teocrito Carlesi Tatiane Cristina Dotta Tania Vanessa Pierfelice Emira D’Amico Stefania Lepore Domenico Tripodi Adriano Piattelli Simonetta D’Ercole Morena Petrini |
| author_sort | Teocrito Carlesi |
| collection | DOAJ |
| description | Background: In this ex vivo study, the aim was to evaluate the effects of ALAD and red light on <i>Enterococcus faecalis</i> in infected root canals using a special intracanal fiber. Methods: A total of 70 extracted, single-rooted teeth were used. The teeth were decoronated at the length of the roots to approximately 15 mm and then instrumented. The apical foramen was sealed by composite resin, and the root canals were infected with a pure culture of <i>E. faecalis</i> ATCC 29212 for eight days at 37 °C. Following the contamination period, the roots were divided into seven groups, including the positive and negative control groups, and treated as follows: ALAD 45 min; red light activation 7 min; ALAD 45 min and red-light activation 7 min; sodium hypochlorite 2.5% 15 min; sodium hypochlorite 1% 15 min. The samples were taken by three sterile paper points, transferred to tubes containing 1 mL of PBS, and immediately processed for the number of colony-forming units and the cell viability by using live/dead. Results: The best treatment is obtained with 2.5% NaOCl. Except for ALAD + red light vs. 1% NaOCl, a statistically significant difference is recorded for all treatments. The combination of 2.5% NaOCl and ALAD + 7 min irradiation produces an evident killing effect on the <i>E. faecalis</i> cells. On the other hand, 1% NaOCl is ineffective for the viability action, with 25% of dead cells stained in red. Conclusions: This ex vivo study shows that ALAD gel with light irradiation is an efficacious protocol that exerts a potent antibacterial activity against <i>E. faecalis</i> in infected root canals. |
| first_indexed | 2024-03-11T08:48:13Z |
| format | Article |
| id | doaj.art-bde2a2a988384928971d88955fc7b3b5 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-11T08:48:13Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-bde2a2a988384928971d88955fc7b3b52023-11-16T20:39:34ZengMDPI AGGels2310-28612023-02-019212510.3390/gels9020125Efficacy of 5% Aminolaevulinic Acid and Red Light on <i>Enterococcus faecalis</i> in Infected Root CanalsTeocrito Carlesi0Tatiane Cristina Dotta1Tania Vanessa Pierfelice2Emira D’Amico3Stefania Lepore4Domenico Tripodi5Adriano Piattelli6Simonetta D’Ercole7Morena Petrini8Department of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Dental Materials and Prosthodontics, School of Dentistry of Ribeirão Preto, University of São Paulo, Sao Paulo 14040-904, BrazilDepartment of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, 66100 Chieti, ItalySchool of Dentistry, Saint Camillus International University for Health Sciences (Unicamillus), 00131 Rome, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, 66100 Chieti, ItalyBackground: In this ex vivo study, the aim was to evaluate the effects of ALAD and red light on <i>Enterococcus faecalis</i> in infected root canals using a special intracanal fiber. Methods: A total of 70 extracted, single-rooted teeth were used. The teeth were decoronated at the length of the roots to approximately 15 mm and then instrumented. The apical foramen was sealed by composite resin, and the root canals were infected with a pure culture of <i>E. faecalis</i> ATCC 29212 for eight days at 37 °C. Following the contamination period, the roots were divided into seven groups, including the positive and negative control groups, and treated as follows: ALAD 45 min; red light activation 7 min; ALAD 45 min and red-light activation 7 min; sodium hypochlorite 2.5% 15 min; sodium hypochlorite 1% 15 min. The samples were taken by three sterile paper points, transferred to tubes containing 1 mL of PBS, and immediately processed for the number of colony-forming units and the cell viability by using live/dead. Results: The best treatment is obtained with 2.5% NaOCl. Except for ALAD + red light vs. 1% NaOCl, a statistically significant difference is recorded for all treatments. The combination of 2.5% NaOCl and ALAD + 7 min irradiation produces an evident killing effect on the <i>E. faecalis</i> cells. On the other hand, 1% NaOCl is ineffective for the viability action, with 25% of dead cells stained in red. Conclusions: This ex vivo study shows that ALAD gel with light irradiation is an efficacious protocol that exerts a potent antibacterial activity against <i>E. faecalis</i> in infected root canals.https://www.mdpi.com/2310-2861/9/2/1255-aminolevulinic acidendodonticsphotodynamic therapyred light<i>Enterococcus faecalis</i> |
| spellingShingle | Teocrito Carlesi Tatiane Cristina Dotta Tania Vanessa Pierfelice Emira D’Amico Stefania Lepore Domenico Tripodi Adriano Piattelli Simonetta D’Ercole Morena Petrini Efficacy of 5% Aminolaevulinic Acid and Red Light on <i>Enterococcus faecalis</i> in Infected Root Canals Gels 5-aminolevulinic acid endodontics photodynamic therapy red light <i>Enterococcus faecalis</i> |
| title | Efficacy of 5% Aminolaevulinic Acid and Red Light on <i>Enterococcus faecalis</i> in Infected Root Canals |
| title_full | Efficacy of 5% Aminolaevulinic Acid and Red Light on <i>Enterococcus faecalis</i> in Infected Root Canals |
| title_fullStr | Efficacy of 5% Aminolaevulinic Acid and Red Light on <i>Enterococcus faecalis</i> in Infected Root Canals |
| title_full_unstemmed | Efficacy of 5% Aminolaevulinic Acid and Red Light on <i>Enterococcus faecalis</i> in Infected Root Canals |
| title_short | Efficacy of 5% Aminolaevulinic Acid and Red Light on <i>Enterococcus faecalis</i> in Infected Root Canals |
| title_sort | efficacy of 5 aminolaevulinic acid and red light on i enterococcus faecalis i in infected root canals |
| topic | 5-aminolevulinic acid endodontics photodynamic therapy red light <i>Enterococcus faecalis</i> |
| url | https://www.mdpi.com/2310-2861/9/2/125 |
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