Delimitation of Some Taxa of <i>Ulnaria</i> and <i>Fragilaria</i> (Bacillariophyceae) Based on Genetic, Morphological Data and Mating Compatibility
<i>Fragilaria</i> and <i>Ulnaria</i> are two closely related diatom genera for which the delimitation and circumscription of several species is unclear. We studied strains isolated from Lake Baikal and compared them with the species from freshwater reservoirs in Europe and As...
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2023-02-01
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| author | Yulia Zakharova Artyom Marchenkov Darya Petrova Yurij Bukin Aleksey Morozov Yekaterina Bedoshvili Yulia Podunay Olga Davidovich Nikolai Davidovich Alexander Bondar Maria Kahlert Yelena Likhoshway |
| author_facet | Yulia Zakharova Artyom Marchenkov Darya Petrova Yurij Bukin Aleksey Morozov Yekaterina Bedoshvili Yulia Podunay Olga Davidovich Nikolai Davidovich Alexander Bondar Maria Kahlert Yelena Likhoshway |
| author_sort | Yulia Zakharova |
| collection | DOAJ |
| description | <i>Fragilaria</i> and <i>Ulnaria</i> are two closely related diatom genera for which the delimitation and circumscription of several species is unclear. We studied strains isolated from Lake Baikal and compared them with the species from freshwater reservoirs in Europe and Asia using phylogenetic and species delimitation methods, microscopy and interclonal crossing experiments. The results of the phylogenetic analyses of the fragments of <i>rbc</i>L and 18S rRNA genes revealed that baikalian <i>F. radians</i> clade was independent from the representatives of the genus from other localities. Among <i>Ulnaria</i> we found the following 18S rRNA phylogenetic tree groups at species level: <i>U. acus</i>, <i>U. ulna</i> and <i>U. danica</i>. Genetic distance between genera varied between 3.9–10.2% substitutions in <i>rbc</i>L gene and 3.2–11.5% in 18S rRNA. The boundary between intraspecies and interspecies polymorphism for studied species of <i>Ulnaria</i> and <i>Fragilaria</i> in these marker genes was around 0.8% substitutions. Morphometric characters of individual strains showed their variability and division into <i>F. radians, U. acus</i> and <i>U. ulna</i> together with <i>U. danica</i>. Strains of <i>U. acus</i> and <i>U. danica</i> from different localities of Europe and Asia were sexually compatible inside the species. Sexual reproduction has never been observed in monoclonal cultures, either between this species or with strains of the <i>Fragilaria.</i> |
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| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-11T08:56:15Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
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| series | Diversity |
| spelling | doaj.art-bde36a66a81c455e837deb13a008a0092023-11-16T20:05:30ZengMDPI AGDiversity1424-28182023-02-0115227110.3390/d15020271Delimitation of Some Taxa of <i>Ulnaria</i> and <i>Fragilaria</i> (Bacillariophyceae) Based on Genetic, Morphological Data and Mating CompatibilityYulia Zakharova0Artyom Marchenkov1Darya Petrova2Yurij Bukin3Aleksey Morozov4Yekaterina Bedoshvili5Yulia Podunay6Olga Davidovich7Nikolai Davidovich8Alexander Bondar9Maria Kahlert10Yelena Likhoshway11Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 3 Ulan-Batorskaya, 664033 Irkutsk, RussiaLimnological Institute, Siberian Branch of the Russian Academy of Sciences, 3 Ulan-Batorskaya, 664033 Irkutsk, RussiaLimnological Institute, Siberian Branch of the Russian Academy of Sciences, 3 Ulan-Batorskaya, 664033 Irkutsk, RussiaLimnological Institute, Siberian Branch of the Russian Academy of Sciences, 3 Ulan-Batorskaya, 664033 Irkutsk, RussiaLimnological Institute, Siberian Branch of the Russian Academy of Sciences, 3 Ulan-Batorskaya, 664033 Irkutsk, RussiaLimnological Institute, Siberian Branch of the Russian Academy of Sciences, 3 Ulan-Batorskaya, 664033 Irkutsk, RussiaT.I. Vyazemsky Karadag Scientific Station of A.O., Kovalevskii Institute of Biology of the Southern Seas of the Russian Academy of Sciences, 24 Nauki Str., Kurortnoe, 298188 Feodosia, RussiaT.I. Vyazemsky Karadag Scientific Station of A.O., Kovalevskii Institute of Biology of the Southern Seas of the Russian Academy of Sciences, 24 Nauki Str., Kurortnoe, 298188 Feodosia, RussiaT.I. Vyazemsky Karadag Scientific Station of A.O., Kovalevskii Institute of Biology of the Southern Seas of the Russian Academy of Sciences, 24 Nauki Str., Kurortnoe, 298188 Feodosia, RussiaGenomics Core Facility of the Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaDepartment of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, SwedenLimnological Institute, Siberian Branch of the Russian Academy of Sciences, 3 Ulan-Batorskaya, 664033 Irkutsk, Russia<i>Fragilaria</i> and <i>Ulnaria</i> are two closely related diatom genera for which the delimitation and circumscription of several species is unclear. We studied strains isolated from Lake Baikal and compared them with the species from freshwater reservoirs in Europe and Asia using phylogenetic and species delimitation methods, microscopy and interclonal crossing experiments. The results of the phylogenetic analyses of the fragments of <i>rbc</i>L and 18S rRNA genes revealed that baikalian <i>F. radians</i> clade was independent from the representatives of the genus from other localities. Among <i>Ulnaria</i> we found the following 18S rRNA phylogenetic tree groups at species level: <i>U. acus</i>, <i>U. ulna</i> and <i>U. danica</i>. Genetic distance between genera varied between 3.9–10.2% substitutions in <i>rbc</i>L gene and 3.2–11.5% in 18S rRNA. The boundary between intraspecies and interspecies polymorphism for studied species of <i>Ulnaria</i> and <i>Fragilaria</i> in these marker genes was around 0.8% substitutions. Morphometric characters of individual strains showed their variability and division into <i>F. radians, U. acus</i> and <i>U. ulna</i> together with <i>U. danica</i>. Strains of <i>U. acus</i> and <i>U. danica</i> from different localities of Europe and Asia were sexually compatible inside the species. Sexual reproduction has never been observed in monoclonal cultures, either between this species or with strains of the <i>Fragilaria.</i>https://www.mdpi.com/1424-2818/15/2/271freshwater systemsdiatomsmolecular phylogenymorphological variabilityreproductive isolation |
| spellingShingle | Yulia Zakharova Artyom Marchenkov Darya Petrova Yurij Bukin Aleksey Morozov Yekaterina Bedoshvili Yulia Podunay Olga Davidovich Nikolai Davidovich Alexander Bondar Maria Kahlert Yelena Likhoshway Delimitation of Some Taxa of <i>Ulnaria</i> and <i>Fragilaria</i> (Bacillariophyceae) Based on Genetic, Morphological Data and Mating Compatibility Diversity freshwater systems diatoms molecular phylogeny morphological variability reproductive isolation |
| title | Delimitation of Some Taxa of <i>Ulnaria</i> and <i>Fragilaria</i> (Bacillariophyceae) Based on Genetic, Morphological Data and Mating Compatibility |
| title_full | Delimitation of Some Taxa of <i>Ulnaria</i> and <i>Fragilaria</i> (Bacillariophyceae) Based on Genetic, Morphological Data and Mating Compatibility |
| title_fullStr | Delimitation of Some Taxa of <i>Ulnaria</i> and <i>Fragilaria</i> (Bacillariophyceae) Based on Genetic, Morphological Data and Mating Compatibility |
| title_full_unstemmed | Delimitation of Some Taxa of <i>Ulnaria</i> and <i>Fragilaria</i> (Bacillariophyceae) Based on Genetic, Morphological Data and Mating Compatibility |
| title_short | Delimitation of Some Taxa of <i>Ulnaria</i> and <i>Fragilaria</i> (Bacillariophyceae) Based on Genetic, Morphological Data and Mating Compatibility |
| title_sort | delimitation of some taxa of i ulnaria i and i fragilaria i bacillariophyceae based on genetic morphological data and mating compatibility |
| topic | freshwater systems diatoms molecular phylogeny morphological variability reproductive isolation |
| url | https://www.mdpi.com/1424-2818/15/2/271 |
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