Research progress of multi-enzyme complexes based on the design of scaffold protein
Abstract Multi-enzyme complexes designed based on scaffold proteins are a current topic in molecular enzyme engineering. They have been gradually applied to increase the production of enzyme cascades, thereby achieving effective biosynthetic pathways. This paper reviews the recent progress in the de...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-10-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40643-023-00695-8 |
| _version_ | 1797454108805824512 |
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| author | Xiangyi Wang Yi Jiang Hongling Liu Haibo Yuan Di Huang Tengfei Wang |
| author_facet | Xiangyi Wang Yi Jiang Hongling Liu Haibo Yuan Di Huang Tengfei Wang |
| author_sort | Xiangyi Wang |
| collection | DOAJ |
| description | Abstract Multi-enzyme complexes designed based on scaffold proteins are a current topic in molecular enzyme engineering. They have been gradually applied to increase the production of enzyme cascades, thereby achieving effective biosynthetic pathways. This paper reviews the recent progress in the design strategy and application of multi-enzyme complexes. First, the metabolic channels in the multi-enzyme complex have been introduced, and the construction strategies of the multi-enzyme complex emerging in recent years have been summarized. Then, the discovered enzyme cascades related to scaffold proteins are discussed, emphasizing on the influence of the linker on the fusion enzyme (fusion protein) and its possible mechanism. This review is expected to provide a more theoretical basis for the modification of multi-enzyme complexes and broaden their applications in synthetic biology. |
| first_indexed | 2024-03-09T15:32:30Z |
| format | Article |
| id | doaj.art-bde52604afdd4382a843992734e9c99a |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-03-09T15:32:30Z |
| publishDate | 2023-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-bde52604afdd4382a843992734e9c99a2023-11-26T12:11:13ZengSpringerOpenBioresources and Bioprocessing2197-43652023-10-0110111810.1186/s40643-023-00695-8Research progress of multi-enzyme complexes based on the design of scaffold proteinXiangyi Wang0Yi Jiang1Hongling Liu2Haibo Yuan3Di Huang4Tengfei Wang5State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences)State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences)State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences)State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences)State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences)State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences)Abstract Multi-enzyme complexes designed based on scaffold proteins are a current topic in molecular enzyme engineering. They have been gradually applied to increase the production of enzyme cascades, thereby achieving effective biosynthetic pathways. This paper reviews the recent progress in the design strategy and application of multi-enzyme complexes. First, the metabolic channels in the multi-enzyme complex have been introduced, and the construction strategies of the multi-enzyme complex emerging in recent years have been summarized. Then, the discovered enzyme cascades related to scaffold proteins are discussed, emphasizing on the influence of the linker on the fusion enzyme (fusion protein) and its possible mechanism. This review is expected to provide a more theoretical basis for the modification of multi-enzyme complexes and broaden their applications in synthetic biology.https://doi.org/10.1186/s40643-023-00695-8Enzyme cascadesSubstrate channelScaffold proteinFusion proteinLinker |
| spellingShingle | Xiangyi Wang Yi Jiang Hongling Liu Haibo Yuan Di Huang Tengfei Wang Research progress of multi-enzyme complexes based on the design of scaffold protein Bioresources and Bioprocessing Enzyme cascades Substrate channel Scaffold protein Fusion protein Linker |
| title | Research progress of multi-enzyme complexes based on the design of scaffold protein |
| title_full | Research progress of multi-enzyme complexes based on the design of scaffold protein |
| title_fullStr | Research progress of multi-enzyme complexes based on the design of scaffold protein |
| title_full_unstemmed | Research progress of multi-enzyme complexes based on the design of scaffold protein |
| title_short | Research progress of multi-enzyme complexes based on the design of scaffold protein |
| title_sort | research progress of multi enzyme complexes based on the design of scaffold protein |
| topic | Enzyme cascades Substrate channel Scaffold protein Fusion protein Linker |
| url | https://doi.org/10.1186/s40643-023-00695-8 |
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