Susceptibility and Multidrug Resistance Patterns of <em>Escherichia coli</em> Isolated from Cloacal Swabs of Live Broiler Chickens in Bangladesh
Antimicrobial resistance is a major health problem, particularly in developing countries like Bangladesh, where there is a paucity of information on resistance patterns and prevalence of antimicrobial determinants. Therefore, the aims of this study were to investigate the prevalence of resistance, i...
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MDPI AG
2019-07-01
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| Online Access: | https://www.mdpi.com/2076-0817/8/3/118 |
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| author | Muha. Ajijur Rahman Al Azad Md. Masudur Rahman Ruhul Amin Mst. Ismat Ara Begum Reinhard Fries Asmaul Husna Ahmed S. Khairalla A.T.M. Badruzzaman Mohamed E. El Zowalaty Kannika Na Lampang Hossam M. Ashour Hafez Mohamed Hafez |
| author_facet | Muha. Ajijur Rahman Al Azad Md. Masudur Rahman Ruhul Amin Mst. Ismat Ara Begum Reinhard Fries Asmaul Husna Ahmed S. Khairalla A.T.M. Badruzzaman Mohamed E. El Zowalaty Kannika Na Lampang Hossam M. Ashour Hafez Mohamed Hafez |
| author_sort | Muha. Ajijur Rahman Al Azad |
| collection | DOAJ |
| description | Antimicrobial resistance is a major health problem, particularly in developing countries like Bangladesh, where there is a paucity of information on resistance patterns and prevalence of antimicrobial determinants. Therefore, the aims of this study were to investigate the prevalence of resistance, including multi-drug resistance (MDR), and the associated genetic determinants in <i>Escherichia coli </i>isolates from cloacal swabs of live broiler chickens in Bangladesh. Altogether, 400 cloacal swabs (200 from Rajshahi and 200 from Dhaka divisions) were randomly collected from individual chickens in 50 broiler farms. <i>E. coli</i> was isolated and identified using conventional bacteriological culture and biochemical methods. The isolates were further confirmed using genus-specific 16S rRNAtargeted polymerase chain reaction (PCR) primers. Antimicrobial susceptibilities and MDR of the isolates against nine different antimicrobial agents (ampicillin, erythromycin, tetracycline, gentamicin, ciprofloxacin, levofloxacin, trimethoprim-sulfamethoxazole, colistin sulphate, and streptomycin) were determined using the Kirby-Bauer disc diffusion method. Resistance determinants of <i>E. coli</i> to ampicillin (<i>bla</i><sub>TEM</sub>), streptomycin (<i>aadA1</i>), erythromycin [<i>ere</i>(A)], trimethoprim (<i>dfrA1</i>), and tetracycline [<i>tet</i>(A), <i>tet</i>(B)] were screened using PCR. Our results showed that all swab samples were positive for <i>E. coli. </i>The isolates were uniformly resistant to ampicillin, tetracycline, streptomycin, ciprofloxacin, erythromycin, and trimethoprim-sulphamethoxazole. The isolates exhibited highest susceptibility to colistin sulphate (73.5%), followed by gentamicin (49%), and levofloxacin (17%). All isolates were resistant to three classes of antibiotics, 204 isolates (51%) were resistant to four classes, and 56 isolates (14%) were resistant to five. The highest prevalence of antimicrobial resistance gene was recorded for tetracycline (<i>tet</i>(A):95.25%; <i>tet</i>(B):95.25%) followed by ampicillin (<i>bla</i><sub>TEM</sub>:91.25%), streptomycin (<i>aadA1</i>:88.25%), erythromycin (<i>ere</i>(A):84.75%), and trimethoprim (<i>dfrA1</i>:65.5%). In conclusion, surveillance for MDR bacteria in poultry is a critical piece of knowledge, which would be useful for optimizing empiric antimicrobial treatments and exploring alternative antimicrobial agents. |
| first_indexed | 2024-04-11T12:18:35Z |
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| spelling | doaj.art-bde776ad509a4b8eb05a2b53d69f1ef52022-12-22T04:24:10ZengMDPI AGPathogens2076-08172019-07-018311810.3390/pathogens8030118pathogens8030118Susceptibility and Multidrug Resistance Patterns of <em>Escherichia coli</em> Isolated from Cloacal Swabs of Live Broiler Chickens in BangladeshMuha. Ajijur Rahman Al Azad0Md. Masudur Rahman1Ruhul Amin2Mst. Ismat Ara Begum3Reinhard Fries4Asmaul Husna5Ahmed S. Khairalla6A.T.M. Badruzzaman7Mohamed E. El Zowalaty8Kannika Na Lampang9Hossam M. Ashour10Hafez Mohamed Hafez11Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet 3100, BangladeshBangladesh Council for Scientific and Industrial Research, Rajshahi 6206, BangladeshDepartment of Animal Husbandry and Veterinary Science, Rajshahi University, Rajshahi 6205, BangladeshInstitute of Food Safety and Food Hygiene, Freie Universitaet Berlin, 14195 Berlin, GermanyDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet 3100, BangladeshDepartment of Microbiology & Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet 3100, BangladeshVirology & Microbiology Research Group, College of Pharmacy, City University College of Ajman, Al Tallah 2, Ajman, P.O. Box 18484, United Arab EmiratesDepartment of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandDepartment of Biological Sciences, College of Arts and Sciences, University of South Florida St. Petersburg, St. Petersburg, FL 33701, USAInstitute of Poultry Diseases, Faculty of Veterinary Medicine, Freie Universitaet Berlin, 14195 Berlin, GermanyAntimicrobial resistance is a major health problem, particularly in developing countries like Bangladesh, where there is a paucity of information on resistance patterns and prevalence of antimicrobial determinants. Therefore, the aims of this study were to investigate the prevalence of resistance, including multi-drug resistance (MDR), and the associated genetic determinants in <i>Escherichia coli </i>isolates from cloacal swabs of live broiler chickens in Bangladesh. Altogether, 400 cloacal swabs (200 from Rajshahi and 200 from Dhaka divisions) were randomly collected from individual chickens in 50 broiler farms. <i>E. coli</i> was isolated and identified using conventional bacteriological culture and biochemical methods. The isolates were further confirmed using genus-specific 16S rRNAtargeted polymerase chain reaction (PCR) primers. Antimicrobial susceptibilities and MDR of the isolates against nine different antimicrobial agents (ampicillin, erythromycin, tetracycline, gentamicin, ciprofloxacin, levofloxacin, trimethoprim-sulfamethoxazole, colistin sulphate, and streptomycin) were determined using the Kirby-Bauer disc diffusion method. Resistance determinants of <i>E. coli</i> to ampicillin (<i>bla</i><sub>TEM</sub>), streptomycin (<i>aadA1</i>), erythromycin [<i>ere</i>(A)], trimethoprim (<i>dfrA1</i>), and tetracycline [<i>tet</i>(A), <i>tet</i>(B)] were screened using PCR. Our results showed that all swab samples were positive for <i>E. coli. </i>The isolates were uniformly resistant to ampicillin, tetracycline, streptomycin, ciprofloxacin, erythromycin, and trimethoprim-sulphamethoxazole. The isolates exhibited highest susceptibility to colistin sulphate (73.5%), followed by gentamicin (49%), and levofloxacin (17%). All isolates were resistant to three classes of antibiotics, 204 isolates (51%) were resistant to four classes, and 56 isolates (14%) were resistant to five. The highest prevalence of antimicrobial resistance gene was recorded for tetracycline (<i>tet</i>(A):95.25%; <i>tet</i>(B):95.25%) followed by ampicillin (<i>bla</i><sub>TEM</sub>:91.25%), streptomycin (<i>aadA1</i>:88.25%), erythromycin (<i>ere</i>(A):84.75%), and trimethoprim (<i>dfrA1</i>:65.5%). In conclusion, surveillance for MDR bacteria in poultry is a critical piece of knowledge, which would be useful for optimizing empiric antimicrobial treatments and exploring alternative antimicrobial agents.https://www.mdpi.com/2076-0817/8/3/118<i>E. coli</i>antibioticsmulti-drug resistancebroiler chickenzoonoticBangladesh |
| spellingShingle | Muha. Ajijur Rahman Al Azad Md. Masudur Rahman Ruhul Amin Mst. Ismat Ara Begum Reinhard Fries Asmaul Husna Ahmed S. Khairalla A.T.M. Badruzzaman Mohamed E. El Zowalaty Kannika Na Lampang Hossam M. Ashour Hafez Mohamed Hafez Susceptibility and Multidrug Resistance Patterns of <em>Escherichia coli</em> Isolated from Cloacal Swabs of Live Broiler Chickens in Bangladesh Pathogens <i>E. coli</i> antibiotics multi-drug resistance broiler chicken zoonotic Bangladesh |
| title | Susceptibility and Multidrug Resistance Patterns of <em>Escherichia coli</em> Isolated from Cloacal Swabs of Live Broiler Chickens in Bangladesh |
| title_full | Susceptibility and Multidrug Resistance Patterns of <em>Escherichia coli</em> Isolated from Cloacal Swabs of Live Broiler Chickens in Bangladesh |
| title_fullStr | Susceptibility and Multidrug Resistance Patterns of <em>Escherichia coli</em> Isolated from Cloacal Swabs of Live Broiler Chickens in Bangladesh |
| title_full_unstemmed | Susceptibility and Multidrug Resistance Patterns of <em>Escherichia coli</em> Isolated from Cloacal Swabs of Live Broiler Chickens in Bangladesh |
| title_short | Susceptibility and Multidrug Resistance Patterns of <em>Escherichia coli</em> Isolated from Cloacal Swabs of Live Broiler Chickens in Bangladesh |
| title_sort | susceptibility and multidrug resistance patterns of em escherichia coli em isolated from cloacal swabs of live broiler chickens in bangladesh |
| topic | <i>E. coli</i> antibiotics multi-drug resistance broiler chicken zoonotic Bangladesh |
| url | https://www.mdpi.com/2076-0817/8/3/118 |
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