Effect of Celecoxib and Infliximab against Multiple Organ Damage Induced by Sepsis in Rats: A Comparative Study
In cases of sepsis, the immune system responds with an uncontrolled release of proinflammatory cytokines and reactive oxygen species. The lungs, kidneys, and liver are among the early impacted organs during sepsis and are a direct cause of mortality. The aim of this study was to compare the effects...
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/2227-9059/10/7/1613 |
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| author | Shaymaa Ramzy Senousy Mahmoud El-Daly Ahmed R. N. Ibrahim Mohamed Montaser A. Khalifa Al-Shaimaa F. Ahmed |
| author_facet | Shaymaa Ramzy Senousy Mahmoud El-Daly Ahmed R. N. Ibrahim Mohamed Montaser A. Khalifa Al-Shaimaa F. Ahmed |
| author_sort | Shaymaa Ramzy Senousy |
| collection | DOAJ |
| description | In cases of sepsis, the immune system responds with an uncontrolled release of proinflammatory cytokines and reactive oxygen species. The lungs, kidneys, and liver are among the early impacted organs during sepsis and are a direct cause of mortality. The aim of this study was to compare the effects of infliximab (IFX) and celecoxib (CLX) on septic rats that went through a cecal ligation and puncture (CLP) surgery to induce sepsis. This study included four groups: sham, CLP (untreated), and CLP-treated with CLX or IFX. The administration of “low dose” CLX or IFX was performed after 2 h following the induction of sepsis. Twenty-four hours following the induction of sepsis, the rats were sacrificed and blood samples were collected to evaluate kidney, liver, and lung injuries. MDA and NOx content, in addition to SOD activity and GSH levels, were evaluated in the tissue homogenates of each group. Tissue samples were also investigated histopathologically. In a separate experiment, the same groups were employed to evaluate the survival of septic rats in a 7-day observation period. The results of this study showed that treatment with either CLX or IFX ameliorated the three organs’ damage compared to septic-untreated rats, decreased oxidative stress, enhanced the antioxidant defense, and reduced serum cytokines. As a result, a higher survival rate resulted: 62.5% and 37.5% after the administration of CLX and IFX, respectively, compared to 0% in the CLP group after 7 days. No significant differences were observed between the two agents in all measured parameters. Histopathological examination confirmed the observed results. In conclusion, CLX and IFX ameliorated lung, kidney, and liver injuries associated with sepsis through anti-inflammatory and antioxidant actions, which correlated to the increase in survival observed with both of them. |
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| last_indexed | 2024-03-09T03:40:44Z |
| publishDate | 2022-07-01 |
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| series | Biomedicines |
| spelling | doaj.art-bde8b590c0674bc6b51f79337d7ac58c2023-12-03T14:41:59ZengMDPI AGBiomedicines2227-90592022-07-01107161310.3390/biomedicines10071613Effect of Celecoxib and Infliximab against Multiple Organ Damage Induced by Sepsis in Rats: A Comparative StudyShaymaa Ramzy Senousy0Mahmoud El-Daly1Ahmed R. N. Ibrahim2Mohamed Montaser A. Khalifa3Al-Shaimaa F. Ahmed4Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minia 61511, EgyptDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minia 61511, EgyptDepartment of Clinical Pharmacy, College of Pharmacy, King Khalid University, Abha 62529, Saudi ArabiaDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minia 61511, EgyptDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minia 61511, EgyptIn cases of sepsis, the immune system responds with an uncontrolled release of proinflammatory cytokines and reactive oxygen species. The lungs, kidneys, and liver are among the early impacted organs during sepsis and are a direct cause of mortality. The aim of this study was to compare the effects of infliximab (IFX) and celecoxib (CLX) on septic rats that went through a cecal ligation and puncture (CLP) surgery to induce sepsis. This study included four groups: sham, CLP (untreated), and CLP-treated with CLX or IFX. The administration of “low dose” CLX or IFX was performed after 2 h following the induction of sepsis. Twenty-four hours following the induction of sepsis, the rats were sacrificed and blood samples were collected to evaluate kidney, liver, and lung injuries. MDA and NOx content, in addition to SOD activity and GSH levels, were evaluated in the tissue homogenates of each group. Tissue samples were also investigated histopathologically. In a separate experiment, the same groups were employed to evaluate the survival of septic rats in a 7-day observation period. The results of this study showed that treatment with either CLX or IFX ameliorated the three organs’ damage compared to septic-untreated rats, decreased oxidative stress, enhanced the antioxidant defense, and reduced serum cytokines. As a result, a higher survival rate resulted: 62.5% and 37.5% after the administration of CLX and IFX, respectively, compared to 0% in the CLP group after 7 days. No significant differences were observed between the two agents in all measured parameters. Histopathological examination confirmed the observed results. In conclusion, CLX and IFX ameliorated lung, kidney, and liver injuries associated with sepsis through anti-inflammatory and antioxidant actions, which correlated to the increase in survival observed with both of them.https://www.mdpi.com/2227-9059/10/7/1613celecoxibinfliximabmultiple organ damagesepsisantioxidant |
| spellingShingle | Shaymaa Ramzy Senousy Mahmoud El-Daly Ahmed R. N. Ibrahim Mohamed Montaser A. Khalifa Al-Shaimaa F. Ahmed Effect of Celecoxib and Infliximab against Multiple Organ Damage Induced by Sepsis in Rats: A Comparative Study Biomedicines celecoxib infliximab multiple organ damage sepsis antioxidant |
| title | Effect of Celecoxib and Infliximab against Multiple Organ Damage Induced by Sepsis in Rats: A Comparative Study |
| title_full | Effect of Celecoxib and Infliximab against Multiple Organ Damage Induced by Sepsis in Rats: A Comparative Study |
| title_fullStr | Effect of Celecoxib and Infliximab against Multiple Organ Damage Induced by Sepsis in Rats: A Comparative Study |
| title_full_unstemmed | Effect of Celecoxib and Infliximab against Multiple Organ Damage Induced by Sepsis in Rats: A Comparative Study |
| title_short | Effect of Celecoxib and Infliximab against Multiple Organ Damage Induced by Sepsis in Rats: A Comparative Study |
| title_sort | effect of celecoxib and infliximab against multiple organ damage induced by sepsis in rats a comparative study |
| topic | celecoxib infliximab multiple organ damage sepsis antioxidant |
| url | https://www.mdpi.com/2227-9059/10/7/1613 |
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