Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity.
To determine if there is a gender dimorphism in the expression of leptin receptors (OB-R170, OB-R128 and OB-R98) and the protein suppressor of cytokine signaling 3 (SOCS3) in human skeletal muscle, the protein expression of OB-R, perilipin A, SOCS3 and alpha-tubulin was assessed by Western blot in m...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2565105?pdf=render |
| _version_ | 1818485471998640128 |
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| author | Borja Guerra Teresa Fuentes Safira Delgado-Guerra Amelia Guadalupe-Grau Hugo Olmedillas Alfredo Santana Jesus Gustavo Ponce-Gonzalez Cecilia Dorado José A L Calbet |
| author_facet | Borja Guerra Teresa Fuentes Safira Delgado-Guerra Amelia Guadalupe-Grau Hugo Olmedillas Alfredo Santana Jesus Gustavo Ponce-Gonzalez Cecilia Dorado José A L Calbet |
| author_sort | Borja Guerra |
| collection | DOAJ |
| description | To determine if there is a gender dimorphism in the expression of leptin receptors (OB-R170, OB-R128 and OB-R98) and the protein suppressor of cytokine signaling 3 (SOCS3) in human skeletal muscle, the protein expression of OB-R, perilipin A, SOCS3 and alpha-tubulin was assessed by Western blot in muscle biopsies obtained from the m. vastus lateralis in thirty-four men (age = 27.1+/-6.8 yr) and thirty-three women (age = 26.7+/-6.7 yr). Basal serum insulin concentration and HOMA were similar in both genders. Serum leptin concentration was 3.4 times higher in women compared to men (P<0.05) and this difference remained significant after accounting for the differences in percentage of body fat or soluble leptin receptor. OB-R protein was 41% (OB-R170, P<0.05) and 163% (OB-R128, P<0.05) greater in women than men. There was no relationship between OB-R expression and the serum concentrations of leptin or 17beta-estradiol. In men, muscle OB-R128 protein was inversely related to serum free testosterone. In women, OB-R98 and OB-R128 were inversely related to total serum testosterone concentration, and OB-R128 to serum free testosterone concentration. SOCS3 protein expression was similar in men and women and was not related to OB-R. In women, there was an inverse relationship between the logarithm of free testosterone and SCOS3 protein content in skeletal muscle (r = -0.46, P<0.05). In summary, there is a gender dimorphism in skeletal muscle leptin receptors expression, which can be partly explained by the influence of testosterone. SOCS3 expression in skeletal muscle is not up-regulated in women, despite very high serum leptin concentrations compared to men. The circulating form of the leptin receptor can not be used as a surrogate measure of the amount of leptin receptors expressed in skeletal muscles. |
| first_indexed | 2024-12-10T16:09:53Z |
| format | Article |
| id | doaj.art-bde9a8c077a64e82a15291890fde90ea |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T16:09:53Z |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bde9a8c077a64e82a15291890fde90ea2022-12-22T01:42:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01310e346610.1371/journal.pone.0003466Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity.Borja GuerraTeresa FuentesSafira Delgado-GuerraAmelia Guadalupe-GrauHugo OlmedillasAlfredo SantanaJesus Gustavo Ponce-GonzalezCecilia DoradoJosé A L CalbetTo determine if there is a gender dimorphism in the expression of leptin receptors (OB-R170, OB-R128 and OB-R98) and the protein suppressor of cytokine signaling 3 (SOCS3) in human skeletal muscle, the protein expression of OB-R, perilipin A, SOCS3 and alpha-tubulin was assessed by Western blot in muscle biopsies obtained from the m. vastus lateralis in thirty-four men (age = 27.1+/-6.8 yr) and thirty-three women (age = 26.7+/-6.7 yr). Basal serum insulin concentration and HOMA were similar in both genders. Serum leptin concentration was 3.4 times higher in women compared to men (P<0.05) and this difference remained significant after accounting for the differences in percentage of body fat or soluble leptin receptor. OB-R protein was 41% (OB-R170, P<0.05) and 163% (OB-R128, P<0.05) greater in women than men. There was no relationship between OB-R expression and the serum concentrations of leptin or 17beta-estradiol. In men, muscle OB-R128 protein was inversely related to serum free testosterone. In women, OB-R98 and OB-R128 were inversely related to total serum testosterone concentration, and OB-R128 to serum free testosterone concentration. SOCS3 protein expression was similar in men and women and was not related to OB-R. In women, there was an inverse relationship between the logarithm of free testosterone and SCOS3 protein content in skeletal muscle (r = -0.46, P<0.05). In summary, there is a gender dimorphism in skeletal muscle leptin receptors expression, which can be partly explained by the influence of testosterone. SOCS3 expression in skeletal muscle is not up-regulated in women, despite very high serum leptin concentrations compared to men. The circulating form of the leptin receptor can not be used as a surrogate measure of the amount of leptin receptors expressed in skeletal muscles.http://europepmc.org/articles/PMC2565105?pdf=render |
| spellingShingle | Borja Guerra Teresa Fuentes Safira Delgado-Guerra Amelia Guadalupe-Grau Hugo Olmedillas Alfredo Santana Jesus Gustavo Ponce-Gonzalez Cecilia Dorado José A L Calbet Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity. PLoS ONE |
| title | Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity. |
| title_full | Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity. |
| title_fullStr | Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity. |
| title_full_unstemmed | Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity. |
| title_short | Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity. |
| title_sort | gender dimorphism in skeletal muscle leptin receptors serum leptin and insulin sensitivity |
| url | http://europepmc.org/articles/PMC2565105?pdf=render |
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