The effect of cyclic nucleotide analog drugs on the mediators release from basophils
Background: The cyclic nucleotides, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), are intracellular second messengers that play an important role in modulating inflammatory cells involved in allergic diseases. In general, cAMP suppresses the activity of immune and...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2015-01-01
|
| Series: | Advanced Biomedical Research |
| Subjects: | |
| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=125;epage=125;aulast=Eskandari |
| _version_ | 1817995714898165760 |
|---|---|
| author | Nahid Eskandari Reza Bastan Seyyed Esfahani Peter T Peachell |
| author_facet | Nahid Eskandari Reza Bastan Seyyed Esfahani Peter T Peachell |
| author_sort | Nahid Eskandari |
| collection | DOAJ |
| description | Background: The cyclic nucleotides, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), are intracellular second messengers that play an important role in modulating inflammatory cells involved in allergic diseases. In general, cAMP suppresses the activity of immune and inflammatory cells. We aim to evaluate the roles of cAMP and cGMP in regulating basophil activity.
Materials and Methods: Basophil-enriched preparations were incubated with analogs and then challenged with anti-IgE or IL-3 (4 or 24 hours). Supernatants were assayed for histamine, IL-4, and IL-13 release. The effects of Sp-8-CPT-cAMPS and Sp-8-CPT-cGMPS on IL-3-dependent mediator release from basophils were determined. The cells were pre-incubated with an analog and then incubated with IL-3 for 24 hours.
Results: Sp-8-CPT-cAMPS was an effective (P < 0.05) inhibitor of IL-4, IL-13, and histamine release from basophils. However, paradoxically, Sp-8-CPT-cGMPS enhanced histamine release and IL-13 generation, but by contrast, had little effect on IL-4 generation. Sp-8-CPT-cGMPS inhibited cytokine generation, but enhanced the release of histamine release to a modest extent.
Conclusion: This study shows that the cAMP/protein kinase A (PKA) pathway may be inhibitory to the IgE- and non-IgE-dependent release of mediators from basophils. |
| first_indexed | 2024-04-14T02:10:39Z |
| format | Article |
| id | doaj.art-bdedb47167924753bbacce64efebe502 |
| institution | Directory Open Access Journal |
| issn | 2277-9175 2277-9175 |
| language | English |
| last_indexed | 2024-04-14T02:10:39Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj.art-bdedb47167924753bbacce64efebe5022022-12-22T02:18:26ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752277-91752015-01-014112512510.4103/2277-9175.158040The effect of cyclic nucleotide analog drugs on the mediators release from basophilsNahid EskandariReza BastanSeyyed EsfahaniPeter T PeachellBackground: The cyclic nucleotides, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), are intracellular second messengers that play an important role in modulating inflammatory cells involved in allergic diseases. In general, cAMP suppresses the activity of immune and inflammatory cells. We aim to evaluate the roles of cAMP and cGMP in regulating basophil activity. Materials and Methods: Basophil-enriched preparations were incubated with analogs and then challenged with anti-IgE or IL-3 (4 or 24 hours). Supernatants were assayed for histamine, IL-4, and IL-13 release. The effects of Sp-8-CPT-cAMPS and Sp-8-CPT-cGMPS on IL-3-dependent mediator release from basophils were determined. The cells were pre-incubated with an analog and then incubated with IL-3 for 24 hours. Results: Sp-8-CPT-cAMPS was an effective (P < 0.05) inhibitor of IL-4, IL-13, and histamine release from basophils. However, paradoxically, Sp-8-CPT-cGMPS enhanced histamine release and IL-13 generation, but by contrast, had little effect on IL-4 generation. Sp-8-CPT-cGMPS inhibited cytokine generation, but enhanced the release of histamine release to a modest extent. Conclusion: This study shows that the cAMP/protein kinase A (PKA) pathway may be inhibitory to the IgE- and non-IgE-dependent release of mediators from basophils.http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=125;epage=125;aulast=EskandariAllergybasophilscytokinedrug |
| spellingShingle | Nahid Eskandari Reza Bastan Seyyed Esfahani Peter T Peachell The effect of cyclic nucleotide analog drugs on the mediators release from basophils Advanced Biomedical Research Allergy basophils cytokine drug |
| title | The effect of cyclic nucleotide analog drugs on the mediators release from basophils |
| title_full | The effect of cyclic nucleotide analog drugs on the mediators release from basophils |
| title_fullStr | The effect of cyclic nucleotide analog drugs on the mediators release from basophils |
| title_full_unstemmed | The effect of cyclic nucleotide analog drugs on the mediators release from basophils |
| title_short | The effect of cyclic nucleotide analog drugs on the mediators release from basophils |
| title_sort | effect of cyclic nucleotide analog drugs on the mediators release from basophils |
| topic | Allergy basophils cytokine drug |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=125;epage=125;aulast=Eskandari |
| work_keys_str_mv | AT nahideskandari theeffectofcyclicnucleotideanalogdrugsonthemediatorsreleasefrombasophils AT rezabastan theeffectofcyclicnucleotideanalogdrugsonthemediatorsreleasefrombasophils AT seyyedesfahani theeffectofcyclicnucleotideanalogdrugsonthemediatorsreleasefrombasophils AT petertpeachell theeffectofcyclicnucleotideanalogdrugsonthemediatorsreleasefrombasophils AT nahideskandari effectofcyclicnucleotideanalogdrugsonthemediatorsreleasefrombasophils AT rezabastan effectofcyclicnucleotideanalogdrugsonthemediatorsreleasefrombasophils AT seyyedesfahani effectofcyclicnucleotideanalogdrugsonthemediatorsreleasefrombasophils AT petertpeachell effectofcyclicnucleotideanalogdrugsonthemediatorsreleasefrombasophils |