Differential metabolic host response to pathogens associated with community-acquired pneumonia
Background: Metabolic changes induced by the host immune response to pathogens found in patients with community-acquired pneumonia (CAP) may provide insight into its pathogenesis. In this study, we characterized differences in the host metabolic response to common CAP-associated pathogens. Method: T...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Metabolism Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589936823000117 |
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| author | Ilona den Hartog Naama Karu Laura B. Zwep G. Paul Voorn Ewoudt M.W. van de Garde Thomas Hankemeier J.G. Coen van Hasselt |
| author_facet | Ilona den Hartog Naama Karu Laura B. Zwep G. Paul Voorn Ewoudt M.W. van de Garde Thomas Hankemeier J.G. Coen van Hasselt |
| author_sort | Ilona den Hartog |
| collection | DOAJ |
| description | Background: Metabolic changes induced by the host immune response to pathogens found in patients with community-acquired pneumonia (CAP) may provide insight into its pathogenesis. In this study, we characterized differences in the host metabolic response to common CAP-associated pathogens. Method: Targeted metabolomic profiling was performed on serum samples obtained from hospitalized CAP patients (n = 119) at admission. We quantified 347 unique metabolites across multiple biochemical classes, including amines, acylcarnitines, and signaling lipids. We evaluated if unique associations between metabolite levels and specific CAP-associated pathogens could be identified. Results: Several acylcarnitines were found to be elevated in C. burnetii and herpes simplex virus and lowered in M. pneumoniae as compared to other pathogens. Phenylalanine and kynurenine were found elevated in L. pneumophila as compared to other pathogens. S-methylcysteine was elevated in patients with M. pneumoniae, and these patients also showed lowered cortisol levels in comparison to almost all other pathogens. For the herpes simplex virus, we observed a unique elevation of eicosanoids and several amines. Many lysophosphatidylcholines showed an altered profile in C. burnetii versus S. pneumoniae, L. pneumophila, and respiratory syncytial virus. Finally, phosphatidylcholines were negatively affected by the influenza virus in comparison to S. pneumoniae. Conclusions: In this exploratory analysis, metabolites from different biochemical classes were found to be altered in serum samples from patients with different CAP-associated pathogens, which may be used for hypothesis generation in studies on differences in pathogen host response and pathogenesis of CAP. |
| first_indexed | 2024-03-13T04:43:42Z |
| format | Article |
| id | doaj.art-bdedbf5fde2f44a9b1bcd455497ae3e0 |
| institution | Directory Open Access Journal |
| issn | 2589-9368 |
| language | English |
| last_indexed | 2024-03-13T04:43:42Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Metabolism Open |
| spelling | doaj.art-bdedbf5fde2f44a9b1bcd455497ae3e02023-06-19T04:29:20ZengElsevierMetabolism Open2589-93682023-06-0118100239Differential metabolic host response to pathogens associated with community-acquired pneumoniaIlona den Hartog0Naama Karu1Laura B. Zwep2G. Paul Voorn3Ewoudt M.W. van de Garde4Thomas Hankemeier5J.G. Coen van Hasselt6Division of Systems Pharmacology & Pharmacy, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the Netherlands; Metabolomics and Analytics Centre, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the NetherlandsMetabolomics and Analytics Centre, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the NetherlandsDivision of Systems Pharmacology & Pharmacy, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the NetherlandsDepartment of Medical Microbiology and Immunology, St. Antonius Hospital, Nieuwegein, the NetherlandsDivision of Pharmacoepidemiology and Clinical Pharmacology, Department of Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands; Department of Clinical Pharmacy, St. Antonius Hospital, Nieuwegein, the NetherlandsMetabolomics and Analytics Centre, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the NetherlandsDivision of Systems Pharmacology & Pharmacy, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the Netherlands; Corresponding author.Background: Metabolic changes induced by the host immune response to pathogens found in patients with community-acquired pneumonia (CAP) may provide insight into its pathogenesis. In this study, we characterized differences in the host metabolic response to common CAP-associated pathogens. Method: Targeted metabolomic profiling was performed on serum samples obtained from hospitalized CAP patients (n = 119) at admission. We quantified 347 unique metabolites across multiple biochemical classes, including amines, acylcarnitines, and signaling lipids. We evaluated if unique associations between metabolite levels and specific CAP-associated pathogens could be identified. Results: Several acylcarnitines were found to be elevated in C. burnetii and herpes simplex virus and lowered in M. pneumoniae as compared to other pathogens. Phenylalanine and kynurenine were found elevated in L. pneumophila as compared to other pathogens. S-methylcysteine was elevated in patients with M. pneumoniae, and these patients also showed lowered cortisol levels in comparison to almost all other pathogens. For the herpes simplex virus, we observed a unique elevation of eicosanoids and several amines. Many lysophosphatidylcholines showed an altered profile in C. burnetii versus S. pneumoniae, L. pneumophila, and respiratory syncytial virus. Finally, phosphatidylcholines were negatively affected by the influenza virus in comparison to S. pneumoniae. Conclusions: In this exploratory analysis, metabolites from different biochemical classes were found to be altered in serum samples from patients with different CAP-associated pathogens, which may be used for hypothesis generation in studies on differences in pathogen host response and pathogenesis of CAP.http://www.sciencedirect.com/science/article/pii/S2589936823000117MetabolomicsImmune responseLipidsAminesCarnitinesBiomarkers |
| spellingShingle | Ilona den Hartog Naama Karu Laura B. Zwep G. Paul Voorn Ewoudt M.W. van de Garde Thomas Hankemeier J.G. Coen van Hasselt Differential metabolic host response to pathogens associated with community-acquired pneumonia Metabolism Open Metabolomics Immune response Lipids Amines Carnitines Biomarkers |
| title | Differential metabolic host response to pathogens associated with community-acquired pneumonia |
| title_full | Differential metabolic host response to pathogens associated with community-acquired pneumonia |
| title_fullStr | Differential metabolic host response to pathogens associated with community-acquired pneumonia |
| title_full_unstemmed | Differential metabolic host response to pathogens associated with community-acquired pneumonia |
| title_short | Differential metabolic host response to pathogens associated with community-acquired pneumonia |
| title_sort | differential metabolic host response to pathogens associated with community acquired pneumonia |
| topic | Metabolomics Immune response Lipids Amines Carnitines Biomarkers |
| url | http://www.sciencedirect.com/science/article/pii/S2589936823000117 |
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