Gut Microbiota Diversity of Preterm Neonates Is Associated With Clostridioides Difficile Colonization
In adults, Clostridioides difficile infections are associated with alterations of the intestinal bacterial populations. Although preterm neonates (PN) are frequently colonized by C. difficile, limited data are available regarding the relationship between C. difficile and the intestinal microbiota of...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.907323/full |
| _version_ | 1811316366630715392 |
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| author | Jeanne Couturier Jeanne Couturier Patricia Lepage Sarah Jolivet Johanne Delannoy Victoria Mesa Pierre-Yves Ancel Pierre-Yves Ancel Jean-Christophe Rozé Marie-José Butel Frédéric Barbut Frédéric Barbut Frédéric Barbut Julio Aires |
| author_facet | Jeanne Couturier Jeanne Couturier Patricia Lepage Sarah Jolivet Johanne Delannoy Victoria Mesa Pierre-Yves Ancel Pierre-Yves Ancel Jean-Christophe Rozé Marie-José Butel Frédéric Barbut Frédéric Barbut Frédéric Barbut Julio Aires |
| author_sort | Jeanne Couturier |
| collection | DOAJ |
| description | In adults, Clostridioides difficile infections are associated with alterations of the intestinal bacterial populations. Although preterm neonates (PN) are frequently colonized by C. difficile, limited data are available regarding the relationship between C. difficile and the intestinal microbiota of this specific population. Therefore, we studied the intestinal microbiota of PN from two multicenter cohorts using high-throughput sequencing of the bacterial 16S rRNA gene. Our results showed that alpha diversity was significantly higher in children colonized by C. difficile than those without colonization. Beta diversity significantly differed between the groups. In multivariate analysis, C. difficile colonization was significantly associated with the absence of postnatal antibiotherapy and higher gestational age. Taxa belonging to the Lachnospiraceae, Enterobacteriaceae, Oscillospiraceae families and Veillonella sp. were positively associated with C. difficile colonization, whereas Bacteroidales and Bifidobacterium breve were negatively associated with C. difficile colonization. After adjustment for covariables, Clostridioides, Rothia, Bifidobacterium, Veillonella, Eisenbergiella genera and Enterobacterales were more abundant in the gut microbiota of colonized children. There was no significant association between C. difficile colonization and necrotizing enterocolitis in PN. Our results suggest that C. difficile colonization in PN is related to the establishment of physiological microbiota. |
| first_indexed | 2024-04-13T11:47:31Z |
| format | Article |
| id | doaj.art-bdeeb93a5ef542ee943ea7802ac32a05 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-13T11:47:31Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-bdeeb93a5ef542ee943ea7802ac32a052022-12-22T02:48:08ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-07-011210.3389/fcimb.2022.907323907323Gut Microbiota Diversity of Preterm Neonates Is Associated With Clostridioides Difficile ColonizationJeanne Couturier0Jeanne Couturier1Patricia Lepage2Sarah Jolivet3Johanne Delannoy4Victoria Mesa5Pierre-Yves Ancel6Pierre-Yves Ancel7Jean-Christophe Rozé8Marie-José Butel9Frédéric Barbut10Frédéric Barbut11Frédéric Barbut12Julio Aires13Université de Paris, Institut national de la santé et de la recherche médicale (INSERM) UMR S-1139 3PHM, Fédération hospitalo-universitaire (FHU) PREMA, F-75006, Paris, FranceNational Reference Laboratory for Clostridioides difficile, Assistance Publique-Hôpitaux de Paris (AP-HP), Saint-Antoine Hospital, Paris, FranceParis-Saclay University, institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAE) AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceInfection Control Unit, Assistance Publique-Hôpitaux de Paris (AP-HP), Saint-Antoine Hospital, Paris, FranceUniversité de Paris, Institut national de la santé et de la recherche médicale (INSERM) UMR S-1139 3PHM, Fédération hospitalo-universitaire (FHU) PREMA, F-75006, Paris, FranceUniversité de Paris, Institut national de la santé et de la recherche médicale (INSERM) UMR S-1139 3PHM, Fédération hospitalo-universitaire (FHU) PREMA, F-75006, Paris, FranceUniversité de Paris, Institut national de la santé et de la recherche médicale (INSERM) UMR 1153, Obstetrical, Perinatal and Pediatric Epidemiology Team (EPOPé), Center of Research in Epidemiology and Statistics (CRESS), Fédération hospitalo-universitaire (FHU) PREMA, Paris, FranceUnité de recherche clinique-Centre d'investigation clinique (URC-CIC) P1419, Hôpitaux universitaires Paris Centre (HUPC), Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, FrancePediatric Intensive Care Unit, Mothers’ and children’s Hospital, Nantes Teaching Hospital, Nantes, FranceUniversité de Paris, Institut national de la santé et de la recherche médicale (INSERM) UMR S-1139 3PHM, Fédération hospitalo-universitaire (FHU) PREMA, F-75006, Paris, FranceUniversité de Paris, Institut national de la santé et de la recherche médicale (INSERM) UMR S-1139 3PHM, Fédération hospitalo-universitaire (FHU) PREMA, F-75006, Paris, FranceNational Reference Laboratory for Clostridioides difficile, Assistance Publique-Hôpitaux de Paris (AP-HP), Saint-Antoine Hospital, Paris, FranceInfection Control Unit, Assistance Publique-Hôpitaux de Paris (AP-HP), Saint-Antoine Hospital, Paris, FranceUniversité de Paris, Institut national de la santé et de la recherche médicale (INSERM) UMR S-1139 3PHM, Fédération hospitalo-universitaire (FHU) PREMA, F-75006, Paris, FranceIn adults, Clostridioides difficile infections are associated with alterations of the intestinal bacterial populations. Although preterm neonates (PN) are frequently colonized by C. difficile, limited data are available regarding the relationship between C. difficile and the intestinal microbiota of this specific population. Therefore, we studied the intestinal microbiota of PN from two multicenter cohorts using high-throughput sequencing of the bacterial 16S rRNA gene. Our results showed that alpha diversity was significantly higher in children colonized by C. difficile than those without colonization. Beta diversity significantly differed between the groups. In multivariate analysis, C. difficile colonization was significantly associated with the absence of postnatal antibiotherapy and higher gestational age. Taxa belonging to the Lachnospiraceae, Enterobacteriaceae, Oscillospiraceae families and Veillonella sp. were positively associated with C. difficile colonization, whereas Bacteroidales and Bifidobacterium breve were negatively associated with C. difficile colonization. After adjustment for covariables, Clostridioides, Rothia, Bifidobacterium, Veillonella, Eisenbergiella genera and Enterobacterales were more abundant in the gut microbiota of colonized children. There was no significant association between C. difficile colonization and necrotizing enterocolitis in PN. Our results suggest that C. difficile colonization in PN is related to the establishment of physiological microbiota.https://www.frontiersin.org/articles/10.3389/fcimb.2022.907323/fullClostridioides (Clostridium) difficilecolonizationpreterm neonatesgut microbiotamicrobial diversity16S rRNA gene sequencing |
| spellingShingle | Jeanne Couturier Jeanne Couturier Patricia Lepage Sarah Jolivet Johanne Delannoy Victoria Mesa Pierre-Yves Ancel Pierre-Yves Ancel Jean-Christophe Rozé Marie-José Butel Frédéric Barbut Frédéric Barbut Frédéric Barbut Julio Aires Gut Microbiota Diversity of Preterm Neonates Is Associated With Clostridioides Difficile Colonization Frontiers in Cellular and Infection Microbiology Clostridioides (Clostridium) difficile colonization preterm neonates gut microbiota microbial diversity 16S rRNA gene sequencing |
| title | Gut Microbiota Diversity of Preterm Neonates Is Associated With Clostridioides Difficile Colonization |
| title_full | Gut Microbiota Diversity of Preterm Neonates Is Associated With Clostridioides Difficile Colonization |
| title_fullStr | Gut Microbiota Diversity of Preterm Neonates Is Associated With Clostridioides Difficile Colonization |
| title_full_unstemmed | Gut Microbiota Diversity of Preterm Neonates Is Associated With Clostridioides Difficile Colonization |
| title_short | Gut Microbiota Diversity of Preterm Neonates Is Associated With Clostridioides Difficile Colonization |
| title_sort | gut microbiota diversity of preterm neonates is associated with clostridioides difficile colonization |
| topic | Clostridioides (Clostridium) difficile colonization preterm neonates gut microbiota microbial diversity 16S rRNA gene sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.907323/full |
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