Association between GPX5 gene polymorphism and selenium concentration in liver and kidney of wild boars from West Pomerania province, Poland

The aim of the experiment was to detect polymorphism in the <em>GPX5</em> gene and to determine associations between individual genotypes and selenium concentration in liver and kidney of wild boars from West Pomerania province in Poland. The polymorphism in <em>GPX5</em> gen...

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Main Authors: Arkadiusz Terman, Kinga Mikolajczyk, Daniel Polasik, Bogumiła Pilarczyk, Malgorzata Bąkowska, Miroslaw Tyra, Grzegorz Żak
Format: Article
Language:English
Published: Taylor & Francis Group 2013-11-01
Series:Italian Journal of Animal Science
Subjects:
Online Access:http://www.aspajournal.it/index.php/ijas/article/view/2899
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author Arkadiusz Terman
Kinga Mikolajczyk
Daniel Polasik
Bogumiła Pilarczyk
Malgorzata Bąkowska
Miroslaw Tyra
Grzegorz Żak
author_facet Arkadiusz Terman
Kinga Mikolajczyk
Daniel Polasik
Bogumiła Pilarczyk
Malgorzata Bąkowska
Miroslaw Tyra
Grzegorz Żak
author_sort Arkadiusz Terman
collection DOAJ
description The aim of the experiment was to detect polymorphism in the <em>GPX5</em> gene and to determine associations between individual genotypes and selenium concentration in liver and kidney of wild boars from West Pomerania province in Poland. The polymorphism in <em>GPX5</em> gene was detected using the PCR-RFLP method with specific primers and the restriction enzyme <em>Hinf</em>I. Two different alleles of the <em>GPX5</em> gene were identified - allele 1B (0.37) and 2B (0.63). The relationships between the <em>GPX5</em> genotypes and concentrations of selenium in liver and kidney were analyzed, revealing statistically significant (P≤0.05) differences between wild boars carrying different <em>GPX5</em> genotypes in both genders. Obtained results indicated that wild boars with the <em>2B2B</em> genotype had the highest selenium concentration in livers and kidneys compared to <em>1B1B</em> and <em>1B2B</em> animals and these differences were statistically significant (P≤0.05).
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spelling doaj.art-bdeede5cce7a4100b145ee0141d8d33d2022-12-21T18:26:22ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2013-11-01124e87e8710.4081/ijas.2013.e872175Association between GPX5 gene polymorphism and selenium concentration in liver and kidney of wild boars from West Pomerania province, PolandArkadiusz Terman0Kinga Mikolajczyk1Daniel Polasik2Bogumiła Pilarczyk3Malgorzata Bąkowska4Miroslaw Tyra5Grzegorz Żak6West Pomeranian University of Technology, SzczecinWest Pomeranian University of Technology, SzczecinWest Pomeranian University of Technology, SzczecinWest Pomeranian University of Technology, SzczecinWest Pomeranian University of Technology, SzczecinNational Research Institute of Animal ProductionNational Research Institute of Animal ProductionThe aim of the experiment was to detect polymorphism in the <em>GPX5</em> gene and to determine associations between individual genotypes and selenium concentration in liver and kidney of wild boars from West Pomerania province in Poland. The polymorphism in <em>GPX5</em> gene was detected using the PCR-RFLP method with specific primers and the restriction enzyme <em>Hinf</em>I. Two different alleles of the <em>GPX5</em> gene were identified - allele 1B (0.37) and 2B (0.63). The relationships between the <em>GPX5</em> genotypes and concentrations of selenium in liver and kidney were analyzed, revealing statistically significant (P≤0.05) differences between wild boars carrying different <em>GPX5</em> genotypes in both genders. Obtained results indicated that wild boars with the <em>2B2B</em> genotype had the highest selenium concentration in livers and kidneys compared to <em>1B1B</em> and <em>1B2B</em> animals and these differences were statistically significant (P≤0.05).http://www.aspajournal.it/index.php/ijas/article/view/2899GPX5 gene, Polymorphism, Selenium, Wild boar.
spellingShingle Arkadiusz Terman
Kinga Mikolajczyk
Daniel Polasik
Bogumiła Pilarczyk
Malgorzata Bąkowska
Miroslaw Tyra
Grzegorz Żak
Association between GPX5 gene polymorphism and selenium concentration in liver and kidney of wild boars from West Pomerania province, Poland
Italian Journal of Animal Science
GPX5 gene, Polymorphism, Selenium, Wild boar.
title Association between GPX5 gene polymorphism and selenium concentration in liver and kidney of wild boars from West Pomerania province, Poland
title_full Association between GPX5 gene polymorphism and selenium concentration in liver and kidney of wild boars from West Pomerania province, Poland
title_fullStr Association between GPX5 gene polymorphism and selenium concentration in liver and kidney of wild boars from West Pomerania province, Poland
title_full_unstemmed Association between GPX5 gene polymorphism and selenium concentration in liver and kidney of wild boars from West Pomerania province, Poland
title_short Association between GPX5 gene polymorphism and selenium concentration in liver and kidney of wild boars from West Pomerania province, Poland
title_sort association between gpx5 gene polymorphism and selenium concentration in liver and kidney of wild boars from west pomerania province poland
topic GPX5 gene, Polymorphism, Selenium, Wild boar.
url http://www.aspajournal.it/index.php/ijas/article/view/2899
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