Effects of arecoline on apoptosis of human periodontal ligament fibroblasts
Objective To observe the effects of arecoline on the related protein expressions in the apoptosis of cul⁃ tured human periodontal ligament fibroblasts (hPDLFs), such as p⁃JNK, p⁃p53, and Bcl⁃2. Methods hPDLFs were iso⁃ lated from human periodontal ligament tissues and expanded in vitro. hPDLFs wer...
| Main Authors: | , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
2017-04-01
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| Series: | 口腔疾病防治 |
| Subjects: | |
| Online Access: | http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2017.04.004 |
| _version_ | 1830416091202977792 |
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| author | WANG Huan⁃ling ZHANG Jian⁃ ping |
| author_facet | WANG Huan⁃ling ZHANG Jian⁃ ping |
| author_sort | WANG Huan⁃ling |
| collection | DOAJ |
| description | Objective To observe the effects of arecoline on the related protein expressions in the apoptosis of cul⁃
tured human periodontal ligament fibroblasts (hPDLFs), such as p⁃JNK, p⁃p53, and Bcl⁃2. Methods hPDLFs were iso⁃
lated from human periodontal ligament tissues and expanded in vitro. hPDLFs were treated with different concentrations
of arecoline (0, 20, 40 and 80 μg/mL) for 12 h. The proliferative activities were evaluated by MTT. The expressions of
p⁃JNK, p⁃p53, and Bcl⁃2 were determined by Western blot. Results Arecoline inhibited cell proliferation and induced
apoptosis protein. The protein level of Bcl⁃2 was decreased, while p⁃JNK and p⁃p53 were increased (P < 0.05). The pro⁃
tein expressions were in a concentration ⁃ dependent manner with arecoline. Conclusion It demonstrates arecoline
might contribute to the apoptosis of hPDLFs, and could destroy periodontal tissues. |
| first_indexed | 2024-12-20T21:17:41Z |
| format | Article |
| id | doaj.art-bdef0cc406684f38bc208775a220441b |
| institution | Directory Open Access Journal |
| issn | 2096-1456 2096-1456 |
| language | zho |
| last_indexed | 2024-12-20T21:17:41Z |
| publishDate | 2017-04-01 |
| publisher | Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases |
| record_format | Article |
| series | 口腔疾病防治 |
| spelling | doaj.art-bdef0cc406684f38bc208775a220441b2022-12-21T19:26:22ZzhoEditorial Department of Journal of Prevention and Treatment for Stomatological Diseases口腔疾病防治2096-14562096-14562017-04-0125422322610.12016/j.issn.2096⁃1456.2017.04.004Effects of arecoline on apoptosis of human periodontal ligament fibroblastsWANG Huan⁃ling0ZHANG Jian⁃ ping1Lushan Mountain Road Community Health Service Center of Tianjin Second Affili⁃ ated Hospital of Tianjin University of TCMObjective To observe the effects of arecoline on the related protein expressions in the apoptosis of cul⁃ tured human periodontal ligament fibroblasts (hPDLFs), such as p⁃JNK, p⁃p53, and Bcl⁃2. Methods hPDLFs were iso⁃ lated from human periodontal ligament tissues and expanded in vitro. hPDLFs were treated with different concentrations of arecoline (0, 20, 40 and 80 μg/mL) for 12 h. The proliferative activities were evaluated by MTT. The expressions of p⁃JNK, p⁃p53, and Bcl⁃2 were determined by Western blot. Results Arecoline inhibited cell proliferation and induced apoptosis protein. The protein level of Bcl⁃2 was decreased, while p⁃JNK and p⁃p53 were increased (P < 0.05). The pro⁃ tein expressions were in a concentration ⁃ dependent manner with arecoline. Conclusion It demonstrates arecoline might contribute to the apoptosis of hPDLFs, and could destroy periodontal tissues.http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2017.04.004ArecolineHuman periodontal ligament fibroblastsApoptosisInhibitionProtein expression |
| spellingShingle | WANG Huan⁃ling ZHANG Jian⁃ ping Effects of arecoline on apoptosis of human periodontal ligament fibroblasts 口腔疾病防治 Arecoline Human periodontal ligament fibroblasts Apoptosis Inhibition Protein expression |
| title | Effects of arecoline on apoptosis of human periodontal ligament fibroblasts |
| title_full | Effects of arecoline on apoptosis of human periodontal ligament fibroblasts |
| title_fullStr | Effects of arecoline on apoptosis of human periodontal ligament fibroblasts |
| title_full_unstemmed | Effects of arecoline on apoptosis of human periodontal ligament fibroblasts |
| title_short | Effects of arecoline on apoptosis of human periodontal ligament fibroblasts |
| title_sort | effects of arecoline on apoptosis of human periodontal ligament fibroblasts |
| topic | Arecoline Human periodontal ligament fibroblasts Apoptosis Inhibition Protein expression |
| url | http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2017.04.004 |
| work_keys_str_mv | AT wanghuanling effectsofarecolineonapoptosisofhumanperiodontalligamentfibroblasts AT zhangjianping effectsofarecolineonapoptosisofhumanperiodontalligamentfibroblasts |