Molecular Serotyping by Phylogenetic Analyses of a 1498bp Segment of the invA Gene of Salmonella
The current gold standard for Salmonella serotyping is costly, labor-intensive and time-consuming. However, proper identification is key to monitor Salmonella transmission and implementation of necessary control measures. The onset of advanced molecular techniques has lessened resource and labor req...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Academy of Sciences Malaysia
2021-04-01
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| Series: | ASM Science Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.32802/asmscj.2020.602 |
| _version_ | 1828010065130422272 |
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| author | Windell L. Rivera Rance Derrick Neri Pavon |
| author_facet | Windell L. Rivera Rance Derrick Neri Pavon |
| author_sort | Windell L. Rivera |
| collection | DOAJ |
| description | The current gold standard for Salmonella serotyping is costly, labor-intensive and time-consuming. However, proper identification is key to monitor Salmonella transmission and implementation of necessary control measures. The onset of advanced molecular techniques has lessened resource and labor requirements; however, it still remains complex, unestablished and plagued with insufficiencies. Hence, a simpler serotyping method with sufficient resolution is needed. In this study, the invA virulence gene, associated with Salmonella invasion into host cells and is considered as a marker for Salmonella detection, was amplified and sequenced among isolates from meat samples in Metro Manila, Philippines. This was followed by sequence alignments with reference sequences (Refseqs), oversaturation and model tests, phylogenetic tree analyses and signal detections. Unfortunately, alignment of a 229bp amplified and sequenced invA gene segment with Refseqs generated little to no base variations and consequently provided insufficient phylogenetic resolution for molecular serotyping (0 of the 17 serotypes tested). However, another segment of 1498bp, outside the amplified region, showed considerable base variation in alignment and consequently resolved a maximum of 13 out of 17 (76.47%) serotypes tested, all generated trees considered. These suggest the potential of the invA virulence gene as a single-gene marker for molecular serotyping of Salmonella through phylogenetic analyses. |
| first_indexed | 2024-04-10T08:48:19Z |
| format | Article |
| id | doaj.art-bdf0442909fd40c9b65baf79b1fd6524 |
| institution | Directory Open Access Journal |
| issn | 1823-6782 |
| language | English |
| last_indexed | 2024-04-10T08:48:19Z |
| publishDate | 2021-04-01 |
| publisher | Academy of Sciences Malaysia |
| record_format | Article |
| series | ASM Science Journal |
| spelling | doaj.art-bdf0442909fd40c9b65baf79b1fd65242023-02-22T04:41:34ZengAcademy of Sciences MalaysiaASM Science Journal1823-67822021-04-011411410.32802/asmscj.2020.602602Molecular Serotyping by Phylogenetic Analyses of a 1498bp Segment of the invA Gene of SalmonellaWindell L. Rivera0Rance Derrick Neri PavonUniversity of the Philippines DilimanThe current gold standard for Salmonella serotyping is costly, labor-intensive and time-consuming. However, proper identification is key to monitor Salmonella transmission and implementation of necessary control measures. The onset of advanced molecular techniques has lessened resource and labor requirements; however, it still remains complex, unestablished and plagued with insufficiencies. Hence, a simpler serotyping method with sufficient resolution is needed. In this study, the invA virulence gene, associated with Salmonella invasion into host cells and is considered as a marker for Salmonella detection, was amplified and sequenced among isolates from meat samples in Metro Manila, Philippines. This was followed by sequence alignments with reference sequences (Refseqs), oversaturation and model tests, phylogenetic tree analyses and signal detections. Unfortunately, alignment of a 229bp amplified and sequenced invA gene segment with Refseqs generated little to no base variations and consequently provided insufficient phylogenetic resolution for molecular serotyping (0 of the 17 serotypes tested). However, another segment of 1498bp, outside the amplified region, showed considerable base variation in alignment and consequently resolved a maximum of 13 out of 17 (76.47%) serotypes tested, all generated trees considered. These suggest the potential of the invA virulence gene as a single-gene marker for molecular serotyping of Salmonella through phylogenetic analyses.https://doi.org/10.32802/asmscj.2020.602salmonellabase variationinva genephylogenetic analysisserotypingtaxonomy |
| spellingShingle | Windell L. Rivera Rance Derrick Neri Pavon Molecular Serotyping by Phylogenetic Analyses of a 1498bp Segment of the invA Gene of Salmonella ASM Science Journal salmonella base variation inva gene phylogenetic analysis serotyping taxonomy |
| title | Molecular Serotyping by Phylogenetic Analyses of a 1498bp Segment of the invA Gene of Salmonella |
| title_full | Molecular Serotyping by Phylogenetic Analyses of a 1498bp Segment of the invA Gene of Salmonella |
| title_fullStr | Molecular Serotyping by Phylogenetic Analyses of a 1498bp Segment of the invA Gene of Salmonella |
| title_full_unstemmed | Molecular Serotyping by Phylogenetic Analyses of a 1498bp Segment of the invA Gene of Salmonella |
| title_short | Molecular Serotyping by Phylogenetic Analyses of a 1498bp Segment of the invA Gene of Salmonella |
| title_sort | molecular serotyping by phylogenetic analyses of a 1498bp segment of the inva gene of salmonella |
| topic | salmonella base variation inva gene phylogenetic analysis serotyping taxonomy |
| url | https://doi.org/10.32802/asmscj.2020.602 |
| work_keys_str_mv | AT windelllrivera molecularserotypingbyphylogeneticanalysesofa1498bpsegmentoftheinvageneofsalmonella AT rancederrickneripavon molecularserotypingbyphylogeneticanalysesofa1498bpsegmentoftheinvageneofsalmonella |