Effects of formalin preservation on egg size of small pelagic fish as major target species in ichthyoplankton surveys
Abstract Preservation is critical for egg and larval samples from ichthyoplankton surveys. As growing interest is directed toward maternal effects on egg quality, historical collections of egg samples in formalin are potentially invaluable materials but concerns about the effects of formalin preserv...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-06-01
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| Series: | Aquaculture, Fish and Fisheries |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/aff2.38 |
| _version_ | 1811333533469245440 |
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| author | Mitsuo Nyuji Michio Yoneda Masahiro Nakamura Mikio Watai Tohya Yasuda Akinori Takasuka |
| author_facet | Mitsuo Nyuji Michio Yoneda Masahiro Nakamura Mikio Watai Tohya Yasuda Akinori Takasuka |
| author_sort | Mitsuo Nyuji |
| collection | DOAJ |
| description | Abstract Preservation is critical for egg and larval samples from ichthyoplankton surveys. As growing interest is directed toward maternal effects on egg quality, historical collections of egg samples in formalin are potentially invaluable materials but concerns about the effects of formalin preservation need to be clarified. The present study examined effects of formalin preservation on egg size of Japanese sardine Sardinops melanostictus, chub mackerel Scomber japonicus and Japanese anchovy Engraulis japonicus over a long period (30–36 months). Overall, the egg sizes slightly decreased in a couple of months after preservation but then recovered to be at a similar level to the live eggs for diameters of spherical eggs of sardine and mackerel and short diameters of ellipsoidal eggs of anchovy, although a caveat lies in unstable and slightly increasing patterns in long diameters of anchovy eggs. No substantial differences were found between 5% and 10% formalin solutions. For practical purposes, we conclude that no correction factors are required for using the measurements of egg samples preserved in formalin over a long period. Assuming the applicability to congeneric species, the information presented here supports the validity of previous studies and will promote future studies on egg size variability in small pelagic fish based on historical collections of egg samples preserved in formalin in various regions of the world. |
| first_indexed | 2024-04-13T16:54:10Z |
| format | Article |
| id | doaj.art-bdf050a5d5474a729a3810a5601db584 |
| institution | Directory Open Access Journal |
| issn | 2693-8847 |
| language | English |
| last_indexed | 2024-04-13T16:54:10Z |
| publishDate | 2022-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aquaculture, Fish and Fisheries |
| spelling | doaj.art-bdf050a5d5474a729a3810a5601db5842022-12-22T02:38:52ZengWileyAquaculture, Fish and Fisheries2693-88472022-06-012320821510.1002/aff2.38Effects of formalin preservation on egg size of small pelagic fish as major target species in ichthyoplankton surveysMitsuo Nyuji0Michio Yoneda1Masahiro Nakamura2Mikio Watai3Tohya Yasuda4Akinori Takasuka5Fisheries Resources Institute/National Research Institute of Fisheries Science (before July 2020) Japan Fisheries Research and Education Agency Yokohama JapanFisheries Technology Institute, Hakatajima Field Station Japan Fisheries Research and Education Agency Imabari JapanFisheries Technology Institute, Hakatajima Field Station Japan Fisheries Research and Education Agency Imabari JapanFisheries Resources Institute/National Research Institute of Fisheries Science (before July 2020) Japan Fisheries Research and Education Agency Yokohama JapanFisheries Resources Institute/National Research Institute of Fisheries Science (before July 2020) Japan Fisheries Research and Education Agency Yokohama JapanFisheries Resources Institute/National Research Institute of Fisheries Science (before July 2020) Japan Fisheries Research and Education Agency Yokohama JapanAbstract Preservation is critical for egg and larval samples from ichthyoplankton surveys. As growing interest is directed toward maternal effects on egg quality, historical collections of egg samples in formalin are potentially invaluable materials but concerns about the effects of formalin preservation need to be clarified. The present study examined effects of formalin preservation on egg size of Japanese sardine Sardinops melanostictus, chub mackerel Scomber japonicus and Japanese anchovy Engraulis japonicus over a long period (30–36 months). Overall, the egg sizes slightly decreased in a couple of months after preservation but then recovered to be at a similar level to the live eggs for diameters of spherical eggs of sardine and mackerel and short diameters of ellipsoidal eggs of anchovy, although a caveat lies in unstable and slightly increasing patterns in long diameters of anchovy eggs. No substantial differences were found between 5% and 10% formalin solutions. For practical purposes, we conclude that no correction factors are required for using the measurements of egg samples preserved in formalin over a long period. Assuming the applicability to congeneric species, the information presented here supports the validity of previous studies and will promote future studies on egg size variability in small pelagic fish based on historical collections of egg samples preserved in formalin in various regions of the world.https://doi.org/10.1002/aff2.38chub mackerelegg sizeformalinJapanese anchovyJapanese sardine |
| spellingShingle | Mitsuo Nyuji Michio Yoneda Masahiro Nakamura Mikio Watai Tohya Yasuda Akinori Takasuka Effects of formalin preservation on egg size of small pelagic fish as major target species in ichthyoplankton surveys Aquaculture, Fish and Fisheries chub mackerel egg size formalin Japanese anchovy Japanese sardine |
| title | Effects of formalin preservation on egg size of small pelagic fish as major target species in ichthyoplankton surveys |
| title_full | Effects of formalin preservation on egg size of small pelagic fish as major target species in ichthyoplankton surveys |
| title_fullStr | Effects of formalin preservation on egg size of small pelagic fish as major target species in ichthyoplankton surveys |
| title_full_unstemmed | Effects of formalin preservation on egg size of small pelagic fish as major target species in ichthyoplankton surveys |
| title_short | Effects of formalin preservation on egg size of small pelagic fish as major target species in ichthyoplankton surveys |
| title_sort | effects of formalin preservation on egg size of small pelagic fish as major target species in ichthyoplankton surveys |
| topic | chub mackerel egg size formalin Japanese anchovy Japanese sardine |
| url | https://doi.org/10.1002/aff2.38 |
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