Evaluation of usefulness of single-strand conformation polymorphism method for rapid detection of rifampicin-resistant mycobacterium tuberculosis
The aim was to evaluate the Single-Strand Conformation Polymorphism method as a potential tool for rapid detection of rifampicin-resistant strains by the use of 39 rifampicin-resistant Mycobacterium tuberculosis strains isolated in Serbia. SSCP analysis on acrylamide gel detected 56.4% of the rifamp...
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| Format: | Article |
| Language: | English |
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Military Health Department, Ministry of Defance, Serbia
2004-01-01
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| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2004/0042-84500402169T.pdf |
| _version_ | 1818342931000459264 |
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| author | Tešić Jelena Lepšanović Zorica Mirović Veljko |
| author_facet | Tešić Jelena Lepšanović Zorica Mirović Veljko |
| author_sort | Tešić Jelena |
| collection | DOAJ |
| description | The aim was to evaluate the Single-Strand Conformation Polymorphism method as a potential tool for rapid detection of rifampicin-resistant strains by the use of 39 rifampicin-resistant Mycobacterium tuberculosis strains isolated in Serbia. SSCP analysis on acrylamide gel detected 56.4% of the rifampicin-resistant M. tuberculosis strains and showed the inability to detect one of the most frequent mutations, TCG®TTG mutation in codon 531 of the rpoB gene, which was shown by automated sequencing. |
| first_indexed | 2024-12-13T16:22:31Z |
| format | Article |
| id | doaj.art-bdf1b742813d4838ab19d8ff9910c684 |
| institution | Directory Open Access Journal |
| issn | 0042-8450 |
| language | English |
| last_indexed | 2024-12-13T16:22:31Z |
| publishDate | 2004-01-01 |
| publisher | Military Health Department, Ministry of Defance, Serbia |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj.art-bdf1b742813d4838ab19d8ff9910c6842022-12-21T23:38:40ZengMilitary Health Department, Ministry of Defance, SerbiaVojnosanitetski Pregled0042-84502004-01-0161216917210.2298/VSP0402169TEvaluation of usefulness of single-strand conformation polymorphism method for rapid detection of rifampicin-resistant mycobacterium tuberculosisTešić JelenaLepšanović ZoricaMirović VeljkoThe aim was to evaluate the Single-Strand Conformation Polymorphism method as a potential tool for rapid detection of rifampicin-resistant strains by the use of 39 rifampicin-resistant Mycobacterium tuberculosis strains isolated in Serbia. SSCP analysis on acrylamide gel detected 56.4% of the rifampicin-resistant M. tuberculosis strains and showed the inability to detect one of the most frequent mutations, TCG®TTG mutation in codon 531 of the rpoB gene, which was shown by automated sequencing.http://www.doiserbia.nb.rs/img/doi/0042-8450/2004/0042-84500402169T.pdfpolymorphismsingle-standed conformationalmycobacterum tuberculosisrifampindrug resistancemicrobial |
| spellingShingle | Tešić Jelena Lepšanović Zorica Mirović Veljko Evaluation of usefulness of single-strand conformation polymorphism method for rapid detection of rifampicin-resistant mycobacterium tuberculosis Vojnosanitetski Pregled polymorphism single-standed conformational mycobacterum tuberculosis rifampin drug resistance microbial |
| title | Evaluation of usefulness of single-strand conformation polymorphism method for rapid detection of rifampicin-resistant mycobacterium tuberculosis |
| title_full | Evaluation of usefulness of single-strand conformation polymorphism method for rapid detection of rifampicin-resistant mycobacterium tuberculosis |
| title_fullStr | Evaluation of usefulness of single-strand conformation polymorphism method for rapid detection of rifampicin-resistant mycobacterium tuberculosis |
| title_full_unstemmed | Evaluation of usefulness of single-strand conformation polymorphism method for rapid detection of rifampicin-resistant mycobacterium tuberculosis |
| title_short | Evaluation of usefulness of single-strand conformation polymorphism method for rapid detection of rifampicin-resistant mycobacterium tuberculosis |
| title_sort | evaluation of usefulness of single strand conformation polymorphism method for rapid detection of rifampicin resistant mycobacterium tuberculosis |
| topic | polymorphism single-standed conformational mycobacterum tuberculosis rifampin drug resistance microbial |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2004/0042-84500402169T.pdf |
| work_keys_str_mv | AT tesicjelena evaluationofusefulnessofsinglestrandconformationpolymorphismmethodforrapiddetectionofrifampicinresistantmycobacteriumtuberculosis AT lepsanoviczorica evaluationofusefulnessofsinglestrandconformationpolymorphismmethodforrapiddetectionofrifampicinresistantmycobacteriumtuberculosis AT mirovicveljko evaluationofusefulnessofsinglestrandconformationpolymorphismmethodforrapiddetectionofrifampicinresistantmycobacteriumtuberculosis |