Variation in Mutation Spectra Among CRISPR/Cas9 Mutagenized Poplars
In an effort to produce reliably contained transgenic trees, we used the CRISPR/Cas9 system to alter three genes expected to be required for normal flowering in poplar (genus Populus). We designed synthetic guide RNAs (sgRNAs) to target the poplar homolog of the floral meristem identity gene, LEAFY...
Main Authors: | Estefania Elorriaga, Amy L. Klocko, Cathleen Ma, Steven H. Strauss |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2018-05-01
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Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00594/full |
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