The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I.
PURPOSE: In a recent study to purify adult T-cell leukemia-lymphoma (ATL) cells from acute-type patients by flow cytometry, three subpopulations were observed in a CD3 versus CD7 plot (H: CD3(high)CD7(high); D: CD3(dim)CD7(dim); L: CD3(dim)CD7(low)). The majority of leukemia cells were enriched in t...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3551918?pdf=render |
| _version_ | 1818554127467151360 |
|---|---|
| author | Seiichiro Kobayashi Yamin Tian Nobuhiro Ohno Koichiro Yuji Tomohiro Ishigaki Masamichi Isobe Mayuko Tsuda Naoki Oyaizu Eri Watanabe Nobukazu Watanabe Kenzaburo Tani Arinobu Tojo Kaoru Uchimaru |
| author_facet | Seiichiro Kobayashi Yamin Tian Nobuhiro Ohno Koichiro Yuji Tomohiro Ishigaki Masamichi Isobe Mayuko Tsuda Naoki Oyaizu Eri Watanabe Nobukazu Watanabe Kenzaburo Tani Arinobu Tojo Kaoru Uchimaru |
| author_sort | Seiichiro Kobayashi |
| collection | DOAJ |
| description | PURPOSE: In a recent study to purify adult T-cell leukemia-lymphoma (ATL) cells from acute-type patients by flow cytometry, three subpopulations were observed in a CD3 versus CD7 plot (H: CD3(high)CD7(high); D: CD3(dim)CD7(dim); L: CD3(dim)CD7(low)). The majority of leukemia cells were enriched in the L subpopulation and the same clone was included in the D and L subpopulations, suggesting clonal evolution. In this study, we analyzed patients with indolent-type ATL and human T-cell leukemia virus type I (HTLV-I) asymptomatic carriers (ACs) to see whether the CD3 versus CD7 profile reflected progression in the properties of HTLV-I-infected cells. EXPERIMENTAL DESIGN: Using peripheral blood mononuclear cells from patient samples, we performed multi-color flow cytometry. Cells that underwent fluorescence-activated cell sorting were subjected to molecular analyses, including inverse long PCR. RESULTS: In the D(%) versus L(%) plot, patient data could largely be categorized into three groups (Group 1: AC; Group 2: smoldering- and chronic-type ATL; and Group 3: acute-type ATL). Some exceptions, however, were noted (e.g., ACs in Group 2). In the follow-up of some patients, clinical disease progression correlated well with the CD3 versus CD7 profile. In clonality analysis, we clearly detected a major clone in the D and L subpopulations in ATL cases and, intriguingly, in some ACs in Group 2. CONCLUSION: We propose that the CD3 versus CD7 plot reflects progression of disease stage in patients infected with HTLV-I. The CD3 versus CD7 profile will be a new indicator, along with high proviral load, for HTLV-I ACs in forecasting disease progression. |
| first_indexed | 2024-12-12T09:35:16Z |
| format | Article |
| id | doaj.art-bdf36de0c5b44e238fcbc7a069ece372 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T09:35:16Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bdf36de0c5b44e238fcbc7a069ece3722022-12-22T00:28:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5372810.1371/journal.pone.0053728The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I.Seiichiro KobayashiYamin TianNobuhiro OhnoKoichiro YujiTomohiro IshigakiMasamichi IsobeMayuko TsudaNaoki OyaizuEri WatanabeNobukazu WatanabeKenzaburo TaniArinobu TojoKaoru UchimaruPURPOSE: In a recent study to purify adult T-cell leukemia-lymphoma (ATL) cells from acute-type patients by flow cytometry, three subpopulations were observed in a CD3 versus CD7 plot (H: CD3(high)CD7(high); D: CD3(dim)CD7(dim); L: CD3(dim)CD7(low)). The majority of leukemia cells were enriched in the L subpopulation and the same clone was included in the D and L subpopulations, suggesting clonal evolution. In this study, we analyzed patients with indolent-type ATL and human T-cell leukemia virus type I (HTLV-I) asymptomatic carriers (ACs) to see whether the CD3 versus CD7 profile reflected progression in the properties of HTLV-I-infected cells. EXPERIMENTAL DESIGN: Using peripheral blood mononuclear cells from patient samples, we performed multi-color flow cytometry. Cells that underwent fluorescence-activated cell sorting were subjected to molecular analyses, including inverse long PCR. RESULTS: In the D(%) versus L(%) plot, patient data could largely be categorized into three groups (Group 1: AC; Group 2: smoldering- and chronic-type ATL; and Group 3: acute-type ATL). Some exceptions, however, were noted (e.g., ACs in Group 2). In the follow-up of some patients, clinical disease progression correlated well with the CD3 versus CD7 profile. In clonality analysis, we clearly detected a major clone in the D and L subpopulations in ATL cases and, intriguingly, in some ACs in Group 2. CONCLUSION: We propose that the CD3 versus CD7 plot reflects progression of disease stage in patients infected with HTLV-I. The CD3 versus CD7 profile will be a new indicator, along with high proviral load, for HTLV-I ACs in forecasting disease progression.http://europepmc.org/articles/PMC3551918?pdf=render |
| spellingShingle | Seiichiro Kobayashi Yamin Tian Nobuhiro Ohno Koichiro Yuji Tomohiro Ishigaki Masamichi Isobe Mayuko Tsuda Naoki Oyaizu Eri Watanabe Nobukazu Watanabe Kenzaburo Tani Arinobu Tojo Kaoru Uchimaru The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I. PLoS ONE |
| title | The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I. |
| title_full | The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I. |
| title_fullStr | The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I. |
| title_full_unstemmed | The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I. |
| title_short | The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I. |
| title_sort | cd3 versus cd7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with htlv i |
| url | http://europepmc.org/articles/PMC3551918?pdf=render |
| work_keys_str_mv | AT seiichirokobayashi thecd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT yamintian thecd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT nobuhiroohno thecd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT koichiroyuji thecd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT tomohiroishigaki thecd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT masamichiisobe thecd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT mayukotsuda thecd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT naokioyaizu thecd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT eriwatanabe thecd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT nobukazuwatanabe thecd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT kenzaburotani thecd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT arinobutojo thecd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT kaoruuchimaru thecd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT seiichirokobayashi cd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT yamintian cd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT nobuhiroohno cd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT koichiroyuji cd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT tomohiroishigaki cd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT masamichiisobe cd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT mayukotsuda cd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT naokioyaizu cd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT eriwatanabe cd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT nobukazuwatanabe cd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT kenzaburotani cd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT arinobutojo cd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi AT kaoruuchimaru cd3versuscd7plotinmulticolorflowcytometryreflectsprogressionofdiseasestageinpatientsinfectedwithhtlvi |