Rational Mutagenesis in the Lid Domain of Ribokinase from <i>E. coli</i> Results in an Order of Magnitude Increase in Activity towards D-arabinose

Rational Mutagenesis in the Lid Domain of Ribokinase from <i>E. coli</i> Results in an Order of Magnitude Increase in Activity towards D-arabinose

Development of efficient approaches for the production of medically important nucleosides is a highly relevant challenge for biotechnology. In particular, cascade synthesis of arabinosides would allow relatively easy production of various cytostatic and antiviral drugs. However, the biocatalyst nece...

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Bibliographic Details
Main Authors: Evgeniy A. Zayats, Ilya V. Fateev, Maria A. Kostromina, Yulia A. Abramchik, Dmitry D. Lykoshin, Daria O. Yurovskaya, Vladimir I. Timofeev, Maria Ya. Berzina, Barbara Z. Eletskaya, Irina D. Konstantinova, Roman S. Esipov
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:International Journal of Molecular Sciences
Subjects:
enzyme genetic improvement
rational design
active site modification
site-directed mutagenesis
ribokinase
cascade synthesis
Online Access:https://www.mdpi.com/1422-0067/23/20/12540

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