Lipid Profile, Antioxidant and Antihypertensive Activity, and Computational Molecular Docking of Diatom Fatty Acids as ACE Inhibitors
Diatoms, as single cell eukaryotic microalgae, are rich sources of lipids, which have either beneficial or detrimental effects on the prevention and treatment of many diseases. Gas chromatography-mass spectrometry (GC-MS) identified diatom lipids with high levels of essential fatty acids (EFAs), esp...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/2/186 |
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| author | Jeeraporn Pekkoh Kittiya Phinyo Theera Thurakit Sureeporn Lomakool Kritsana Duangjan Khomsan Ruangrit Chayakorn Pumas Supat Jiranusornkul Wipawadee Yooin Benjamas Cheirsilp Wasu Pathom-aree Sirasit Srinuanpan |
| author_facet | Jeeraporn Pekkoh Kittiya Phinyo Theera Thurakit Sureeporn Lomakool Kritsana Duangjan Khomsan Ruangrit Chayakorn Pumas Supat Jiranusornkul Wipawadee Yooin Benjamas Cheirsilp Wasu Pathom-aree Sirasit Srinuanpan |
| author_sort | Jeeraporn Pekkoh |
| collection | DOAJ |
| description | Diatoms, as single cell eukaryotic microalgae, are rich sources of lipids, which have either beneficial or detrimental effects on the prevention and treatment of many diseases. Gas chromatography-mass spectrometry (GC-MS) identified diatom lipids with high levels of essential fatty acids (EFAs), especially polyunsaturated FAs (PUFAs) containing both omega-3 and omega-6. Nutritional values of FAs indicated possible applications in the pharmaceutical, nutraceutical, and functional food industries. Diatom FAs showed antioxidative potential on harmful radicals by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging, with high inhibition of the angiotensin-converting enzyme (ACE) that causes cardiovascular disease (CVD) and hypertension. A computational molecular docking simulation confirmed the inhibition mechanisms of FAs on ACE, with comparable levels of binding free energy to chemically synthesized ACE drugs. Findings suggested that diatom lipids showed potential for use as alternative ACE inhibitors or food supplement for CVD prevention. |
| first_indexed | 2024-03-09T22:45:48Z |
| format | Article |
| id | doaj.art-bdf88aa956814a69916fe3e04c309e65 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T22:45:48Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-bdf88aa956814a69916fe3e04c309e652023-11-23T18:29:44ZengMDPI AGAntioxidants2076-39212022-01-0111218610.3390/antiox11020186Lipid Profile, Antioxidant and Antihypertensive Activity, and Computational Molecular Docking of Diatom Fatty Acids as ACE InhibitorsJeeraporn Pekkoh0Kittiya Phinyo1Theera Thurakit2Sureeporn Lomakool3Kritsana Duangjan4Khomsan Ruangrit5Chayakorn Pumas6Supat Jiranusornkul7Wipawadee Yooin8Benjamas Cheirsilp9Wasu Pathom-aree10Sirasit Srinuanpan11Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandScience and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, ThailandScience and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandLaboratory for Molecular Design and Simulation (LMDS), Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandLaboratory for Molecular Design and Simulation (LMDS), Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandCenter of Excellence in Innovative Biotechnology for Sustainable Utilization of Bioresources, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDiatoms, as single cell eukaryotic microalgae, are rich sources of lipids, which have either beneficial or detrimental effects on the prevention and treatment of many diseases. Gas chromatography-mass spectrometry (GC-MS) identified diatom lipids with high levels of essential fatty acids (EFAs), especially polyunsaturated FAs (PUFAs) containing both omega-3 and omega-6. Nutritional values of FAs indicated possible applications in the pharmaceutical, nutraceutical, and functional food industries. Diatom FAs showed antioxidative potential on harmful radicals by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging, with high inhibition of the angiotensin-converting enzyme (ACE) that causes cardiovascular disease (CVD) and hypertension. A computational molecular docking simulation confirmed the inhibition mechanisms of FAs on ACE, with comparable levels of binding free energy to chemically synthesized ACE drugs. Findings suggested that diatom lipids showed potential for use as alternative ACE inhibitors or food supplement for CVD prevention.https://www.mdpi.com/2076-3921/11/2/186fatty acidsantioxidantantihypertensivenutritional quality indicesmolecular dockingmicroalgal diatom |
| spellingShingle | Jeeraporn Pekkoh Kittiya Phinyo Theera Thurakit Sureeporn Lomakool Kritsana Duangjan Khomsan Ruangrit Chayakorn Pumas Supat Jiranusornkul Wipawadee Yooin Benjamas Cheirsilp Wasu Pathom-aree Sirasit Srinuanpan Lipid Profile, Antioxidant and Antihypertensive Activity, and Computational Molecular Docking of Diatom Fatty Acids as ACE Inhibitors Antioxidants fatty acids antioxidant antihypertensive nutritional quality indices molecular docking microalgal diatom |
| title | Lipid Profile, Antioxidant and Antihypertensive Activity, and Computational Molecular Docking of Diatom Fatty Acids as ACE Inhibitors |
| title_full | Lipid Profile, Antioxidant and Antihypertensive Activity, and Computational Molecular Docking of Diatom Fatty Acids as ACE Inhibitors |
| title_fullStr | Lipid Profile, Antioxidant and Antihypertensive Activity, and Computational Molecular Docking of Diatom Fatty Acids as ACE Inhibitors |
| title_full_unstemmed | Lipid Profile, Antioxidant and Antihypertensive Activity, and Computational Molecular Docking of Diatom Fatty Acids as ACE Inhibitors |
| title_short | Lipid Profile, Antioxidant and Antihypertensive Activity, and Computational Molecular Docking of Diatom Fatty Acids as ACE Inhibitors |
| title_sort | lipid profile antioxidant and antihypertensive activity and computational molecular docking of diatom fatty acids as ace inhibitors |
| topic | fatty acids antioxidant antihypertensive nutritional quality indices molecular docking microalgal diatom |
| url | https://www.mdpi.com/2076-3921/11/2/186 |
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